Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Fisheries
Rongchao He, Yanfei Zuo, Lingmin Zhao, Ying Ma, Qingpi Yan, Lixing Huang
Summary: Copper is crucial for cellular functions but high levels can damage biological macromolecules. Bacterial infection leads to copper accumulation and copper resistance mechanisms play a vital role in bacterial virulence, particularly seen in Vibrio alginolyticus. Silencing of key copper resistance genes cusS and cusR unexpectedly increased virulence factors, while CusR was found to regulate both copper tolerance and virulence genes. Activation of the CusSR system by copper stress reduces pathogenesis in V. alginolyticus.
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Plant Sciences
Xiaoli Liu, Jun Cai, Xiaoxu Li, Feng Yu, Dousheng Wu
Summary: The article hypothesizes that type III effectors manipulate the microbiota composition by mediating ternary pathogen-plant-microbiota interactions, providing new insights for studying the biological functions of type III effectors and developing new strategies for plant disease control.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Fisheries
Qiuting Zhuang, Fa Dai, Yina Shao, Chenghua Li, Weiwei Zhang
Summary: The T3SS ATPase gene VscN in Vibrio splendidus AJ01 plays a crucial role in pathogenesis, affecting the survival rate of Apostichopus japonicus. The expression and secretion of the T3SS effector Hop are attenuated in the Delta vscN mutant strain, indicating a dependency on VscN.
Article
Fisheries
Chen Wang, Peng-Fei Li, Da-Gang Hu, Hui Wang
Summary: In this study, the impact of Clostridium butyricum feeding on intestinal microorganisms and protection against Vibrio alginolyticus infection in Penaeus vannamei was evaluated. The results showed that Clostridium butyricum significantly altered the abundance of intestinal microbial populations and reduced Vibrio spp. Furthermore, the Clostridium butyricum-fed group exhibited improved survival rate, reduced pathological changes, and enhanced immune response against V. alginolyticus infection compared to the control group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Jun Yang, Xiao-li Yang, Yu-bin Su, Xuan-xian Peng, Hui Li
Summary: Vaccines are safe and effective in controlling bacterial diseases in the aquaculture industry and align with green farming. A novel approach of preparing live-attenuated V. alginolyticus vaccine using high magnesium concentration to attenuate virulence was developed. Metabolomic analysis revealed enhanced TCA cycle and increased fumarate as crucial pathways and biomarkers in vaccine-mediated and survival fish.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yaqiu Zhang, Yiqin Deng, Juan Feng, Zhixun Guo, Haoxiang Chen, Baotun Wang, Jianmei Hu, Ziyang Lin, Youlu Su
Summary: The research has shown that the VscCD protein of the type III secretion system plays a significant role in the pathogenicity of Vibrio harveyi. Deletion of VscCD leads to reduced resistance to acid stress, H2O2, and antibiotics, as well as decreased host mortality.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Fan Da, Xiaoju Wan, Guixiang Lin, Jichang Jian, Shuanghu Cai
Summary: In this study, the fliR deletion mutant Delta fliR of V.alginolyticus was constructed and evaluated, and the gene expression differences between wild-type and Delta fliR were analyzed by transcriptomics. Results showed that Delta fliR significantly affected cell motility and related pathways. Additionally, Delta fliR as a live attenuated vaccine demonstrated high protective efficacy and immune stimulation in groupers.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Feifei Wang, Lin Huang, Meiqiu Liao, Wenna Dong, Can Liu, Yuan Liu, Qingjian Liang, Weina Wang
Summary: After Vibrio alginolyticus infection, DNA damage, autophagy and apoptosis in hemocytes of Penaeus vannamei significantly increased, with multiple signaling pathways involved in regulating the shrimp's response to bacterial infection. The study identified numerous mRNA-miRNA interactions that provide insight into the defense mechanism during the antimicrobial process of P. vannamei.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Benjamin Kostiuk, Francis J. Santoriello, Laura Diaz-Satizabal, Fabiana Bisaro, Kyung-Jo Lee, Anna N. Dhody, Daniele Provenzano, Daniel Unterweger, Stefan Pukatzki
Summary: It was found that classical Vibrio cholerae underwent sequential mutations in T6SS genetic determinants, making them disadvantaged in competition with the 7th pandemic El Tor biotype strains.
NATURE COMMUNICATIONS
(2021)
Article
Environmental Sciences
Huiming Tan, Fan Da, Guixiang Lin, Xiaoju Wan, Jichang Jian, Shuanghu Cai
Summary: VAGM001033 is an active diguanylate cyclase, and its mutant Delta VAGM001033 showed altered physiology and pathogenicity. RNA-seq analysis identified differentially expressed genes, and the mutant provided immune protection.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Fisheries
Xiongchao Ma, Fei Zhu
Summary: Myosin-9 plays a key role in the immune response of S. paramamosain against WSSV and V. alginolyticus, affecting the expression of immune-related genes and the antiviral capacity of crab hemocytes when knocked down.
