期刊
FISH & SHELLFISH IMMUNOLOGY
卷 35, 期 4, 页码 1301-1308出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.08.001
关键词
Fish; Treatment; Monogenean; Gene expression; Susceptibility
资金
- Natural Science Foundation of China [31072242]
Praziquantel and sanguinarine have been demonstrated positive therapeutic effects on monogenean Dactylogyrus intermedius; however, few studies have considered the post impacts of these antiparasitic chemicals on host after repelling the parasites. The changes of expression of selected immune genes (CCL-1, CXCL-8, IL-1 beta-1, IL-1 beta-2, TNF alpha-1, TNF alpha-2 and TGF-beta) in gill, kidney and spleen and bacterial loads of Aeromonas hydrophila in gill, kidney, spleen and liver following bath administration of these antiparasitic chemicals were evaluated. The results showed that praziquantel and sanguinarine up-regulated to varying degrees of CXCL-8, IL-1 beta-1, IL-1 beta-2, TNF alpha-1 and TNF alpha-2 in gill, kidney and spleen. They both decreased the CCL-1 expression in gill while increased it in kidney and spleen. However, in all the tested tissues, the expression of TGF-beta decreased in praziquantel treated goldfish whereas that increased in sanguinarine treated goldfish. The A. hydrophila challenge test showed that the praziquantel treatment enhanced the susceptibility to A. hydrophila while sanguinarine treatment decreased the susceptibility, as compared with the non-treated group. Overall, the results indicate that bath administration of praziquantel and sanguinarine modulates the immune related genes in goldfish and these may, to some extent, affect their ability to resist bacterial pathogens. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据