期刊
FISH & SHELLFISH IMMUNOLOGY
卷 34, 期 6, 页码 1421-1431出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2013.02.030
关键词
p38 MAPK; Litopenaeus vannamei; Antimicrobial peptides (AMPs); RNA interference (RNAi)
资金
- National Natural Science Foundation of China [U1131002, 201242000 4103030]
- National High Technology Research and Development Program of China (973 Program) [2012CB114401]
- China Agriculture Research System
- Special Fund for Agro-scientific Research in the Public Interest [201103034]
- Foundation of Administration of Ocean and Fisheries of Guangdong Province [A201101B02]
- State Key Laboratory of Biocontrol [SKLBC09K04]
- Foundation of Science and Technology Bureau of Guangdong Province [2011A020102002, 2009A020102002]
p38 mitogen-activated protein kinases (MAPKs) are broadly expressed from yeasts to mammals, and are involved in the regulation of cells responsible to various extracellular stimuli. In this study, a p38 MAPK gene (designated as Lvp38) from Litopenaeus vannamei, was cloned and characterized. It contained the conserved structures of a Thr-Gly-Tyr (TGY) motif and a substrate-binding site, Ala-Thr-Arg-Trp (ATRW). The tissue distribution patterns showed that Lvp38 was widely expressed in all examined tissues, with the highest expression in hemocytes, nerves, and intestines. Quantitative real-time PCR revealed that Lvp38 was upregulated in gills and hemocytes after infection with the Gram-negative Vibrio alginolyticus and the Gram-positive Staphylococcus aureus. Reporter gene assays indicated that Lvp38 activated the expression of antimicrobial peptides (AMPs) of Drosophila and shrimp. Knockdown of Lvp38 by RNA interference (RNAi) resulted in a higher mortality of L. vannamei under V. alginolyticus and S. aureus infection, as well as a reduction in the expression of three shrimp AMP genes, namely, PEN4, crustin, and ALF2. Taken together, our data indicated that Lvp38 played a role in defending against bacterial infections. (C) 2013 Elsevier Ltd. All rights reserved.
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