Article
Biochemistry & Molecular Biology
Hiroyuki Inaba, Yuzo Iwata, Takashi Suzuki, Moemi Horiuchi, Ryohei Surugaya, Shigeho Ijiri, Ai Uchiyama, Ryoko Takano, Seiji Hara, Takashi Yazawa, Takeshi Kitano
Summary: In this study, the effects of the phytoestrogens genistein and daidzein on sex differentiation and sex-specific genes in Japanese eels were examined. Genistein was found to induce feminization more efficiently than daidzein, by altering the molecular pathways responsible for eel sex differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Akihide Kasai, Aya Yamazaki, Hyojin Ahn, Hiroki Yamanaka, Satoshi Kameyama, Reiji Masuda, Nobuyuki Azuma, Shingo Kimura, Tatsuro Karaki, Yuko Kurokawa, Yoh Yamashita
Summary: The distribution of Japanese eels was investigated using environmental DNA (eDNA) monitoring, revealing high eDNA concentrations in rivers on the Pacific side and lower concentrations on the Sea of Japan side. The study suggests that the distribution of Japanese eels is mainly determined by maritime larval transport, with their survival and growth dependent on food abundance in rivers.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Cell Biology
Moemi Horiuchi, Seishi Hagihara, Manabu Kume, Daichi Chushi, Yuya Hasegawa, Hikaru Itakura, Yoh Yamashita, Shinji Adachi, Shigeho Ijiri
Summary: This study provides the first demonstration of the entire process of natural gonadal sex differentiation in eels at both molecular and morphological levels. The findings suggest that sex differentiation in eels is triggered by growth rather than aging.
Article
Reproductive Biology
Xiao Jian Lai, Shuai Peng, Yi Lei Wang
Summary: This study investigates the dynamic molecular regulation of follicular development in Anguilla japonica, focusing on the asynchronous development of follicles within the same individual. The findings suggest that steroid hormone synthesis and nutrient absorption play important roles in follicular development and maturation.
Article
Fisheries
Xiaojian Lai, Shuai Peng, Jianjun Feng, Pengfei Zou, Yilei Wang
Summary: This study examines immune function changes in the Japanese eel during artificial ovarian maturation, revealing differentially expressed genes associated with the immune system and suggesting the potential roles of the interleukin-17 pathway and IgM in spawning.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yung-Sen Huang, Wen-Chih Cheng, Chung-Yen Lin
Summary: Androgens stimulate ovarian development in eels, with the initial ovarian status potentially influencing sensitivity to 17MT treatment. Genes related to neuronal activities or neurogenesis may play an essential role in the positive effects of treatment.
MARINE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nobuto Kaneko, Takashi Ishikawa, Kazuharu Nomura
Summary: The study investigated the regulatory mechanism of growth in Japanese eel larvae by examining the expression of growth-related genes under different nutritional conditions. The results suggest that growth hormone (GH), insulin-like growth factors (IGFs), and IGF binding protein (IGFBP) play important roles in postnatal growth. Additionally, transforming growth factor-beta 3 (TGF-03) appears to be a key modulator of muscle growth and body component synthesis. The expression levels of these genes were found to be influenced by fasting and refeeding, and factors such as body size and nutritional status were correlated with their expression levels.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2023)
Article
Fisheries
Yiqun Xiao, Liqun Wu, Le He, Yijun Tang, Songlin Guo, Shaowei Zhai
Summary: This study investigated the interaction of differentially expressed genes (DEGs) between Edwardsiella anguillarum (Ea) and Anguilla anguilla (Aa) during infection using dual RNA sequencing. The results showed severe bleeding and thrombus formation in the liver of the infected group. Gene ontology and KEGG analysis revealed that upregulated DEGs in the pathogen were mainly involved in migration, colonization, and biofilm formation, while downregulated DEGs were mainly associated with metabolism and information transduction. The interaction network analysis identified important pathogen and host proteins involved in infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Jinho Bae, Seunghyung Lee, Mohammad Moniruzzaman, Ali Hamidoghli, Wonsuk Choi, Seunghan Lee, Taesun Min, Shin-Kwon Kim, Sungchul C. Bai
Summary: The study investigated the effects of different diets containing fish meal and fish meal analog on juvenile Japanese eel, determined the optimum level of fish meal analog as a substitute for fish meal protein, and evaluated the immunostimulant effects of natural feed additives. The results showed that fish meal analog could replace up to 10% of fish meal protein without compromising growth and health of the fish, and the additives could improve the replacing rate of fish meal analog without affecting the fish's growth and health.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Yung-Sen Huang, Chung-Yen Lin, Wen-Chih Cheng
Summary: The study conducted RNA sequencing on eels with different ovarian developments treated with androgen, revealing the presence of ePAV in Japanese eels. This indicates the importance of genetic differential expression on phenotypic variations induced by androgen, with a transcriptomic approach allowing for multiple layers of genomic data to be extracted.
MARINE BIOTECHNOLOGY
(2021)
Article
Fisheries
Xuangang Wang, Xiangfu Kong, Xiaobing Liu, Xubo Wang, Zhigang Wang, Jinxiang Liu, Quanqi Zhang, Haiyang Yu
Summary: Pyroptosis is a caspase-1-dependent form of programmed cell death characterized by cell lysis and inflammatory cytokine release. This study demonstrates that E. tarda infection triggers pyroptosis in macrophages of Japanese flounder, playing a vital role in the immune response. Treatment with a caspase-1 inhibitor significantly reduces pyroptosis, indicating the association of caspase-1 with pyroptosis.
Article
Fisheries
Ying Huang, Ruxiang Ge, Gege Lou, Nengzuo Jiang, Xiaoming Zhu, Yazhe Guo, Haokun Liu, Xuanyu Liu, Xinhua Chen
Summary: The study investigated the effects of dietary Coenzyme Q10 (CoQ10) on the growth performance, body composition, digestive enzyme activity, antioxidant capacity, intestinal histology, immune-antioxidant gene expression, and disease resistance of juvenile European eel. The results showed that dietary CoQ10 supplementation did not significantly affect the overall body weight, survival rate, weight gain, feed rate, viscerosomatic index, or hepatosomatic index. However, the group supplemented with 120 mg/kg CoQ10 showed the highest body weight, weight gain, and survival rate. Additionally, this group exhibited improved feed efficiency, reduced lipid content, and enhanced antioxidant capacity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Wenxuan He, Liqun Wu, Senlin Li, Songlin Guo
Summary: This study focused on the regulation of long noncoding RNAs (lncRNAs) in the process of Edwardsiella anguillarum infection and European eel anti-Ea infection after the immunization of a recombinant OmpA. Through high-throughput transcriptomics, 13405 lncRNAs were identified in different comparison groups. Functional analysis indicated that lncRNAs target genes involved in processes such as single organism process, signalling, and response to stimulus, with enriched pathways related to cell adhesion molecules. Additionally, an interaction network revealed specific lncRNAs playing crucial roles in anti-Ea infection.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Peng Lin, Ming Xu, Qiuhua Yang, Minxia Chen, Songlin Guo
Summary: This study investigates the molecular mechanism of nonspecific immune enhancement in subunit fishery vaccine using Freund's complete (FCA) and incomplete adjuvants (FIA). Through RNA-seq analysis, key KEGG pathways and differential expressed genes (DEGs) were identified in the spleen of European eel inoculated with FCA and FIA. The result showed that FCIA has a higher relative percent survival (RPS) compared to Con_inf group, and an increased SOD activity in the liver and spleen.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yutaka Kawakami
Summary: Japanese eels store lipids in the peritoneal cavity during metamorphosis and utilize triglycerides as an energy source. This strategy allows Japanese eels to maintain energy levels even when they are not feeding.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Integrative & Complementary Medicine
Bo-Ra Jeon, Muhammad Irfan, Seung Eun Lee, Jeong Hoon Lee, Man Hee Rhee
Summary: The study found that Rumex acetosella extract has inhibitory effects on platelet activity and can exert its effects by modulating signaling pathways. This suggests that Rumex acetosella may have a protective role in the development of cardiovascular diseases.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2022)
Article
Plant Sciences
Muhammad Irfan, Yuan Yee Lee, Ki-Ja Lee, Sung Dae Kim, Man Hee Rhee
Summary: This study investigated the antiplatelet effects of red ginseng extract (RGE) and fermented red ginseng extract (FRG) and found that both extracts can inhibit platelet aggregation and calcium mobilization, indicating their potential for maintaining cardiovascular health and preventing platelet-related cardiovascular disorders.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Plant Sciences
H. M. Arif Ullah, Evelyn Saba, Yuan Yee Lee, Seung-Bok Hong, Sun-Hee Hyun, Yi-Seong Kwak, Chae-Kyu Park, Sung Dae Kim, Man Hee Rhee
Summary: This study investigated the effects of Rg3-RGE and PT on a mouse model of UC and found that the combination treatment significantly improved colon length, body weight, and disease activity index, reduced histological injury, decreased levels of nitric oxide, inflammatory mediators, and cytokines. Also, the combination treatment suppressed NLRP3 inflammasome and NF-κB activity, and increased the number of immune cell subtypes.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Environmental Sciences
Dereje Tassew, Susan Fort, Yohannes Mebratu, Jacob McDonald, Hong Wei Chu, Hans Petersen, Yohannes Tesfaigzi
Summary: Exposure to wood smoke (WS) increases mucin expression more efficiently than exposure to cigarette smoke (CS), especially in a p53 pathway-dependent manner. The WS constituent oxalate can enhance mucin expression, particularly in individuals with a p53 arginine genotype, revealing potential mechanisms for increased risk of chronic bronchitis. Identifying responsible compounds and genetic variations could help estimate pollutant-related risks and improve lung disease management strategies.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2022)
Article
Immunology
Yuan Yee Lee, H. M. Arif Ullah, Lee Su Ha, Sung Dae Kim, Bong-Sik Yun, Man Hee Rhee
Summary: This study investigated the therapeutic efficacy of isopanepoxydone (ISO) isolated from Panus rudis against respiratory complications caused by particulate matter. The results showed that ISO effectively inhibited nitric oxide production, pro-inflammatory mediators, and cytokines in murine alveolar macrophages (MH-S cells). ISO also enhanced the expression of nuclear factor erythroid 2-related factor 2 and suppressed proteins in the inflammasome pathway, indicating its potential as a treatment for respiratory inflammation.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Muhammad Irfan, Tae-Hyung Kwon, Hyuk-Woo Kwon, Man Hee Rhee
Summary: Dieckol, a phlorotannin found in seaweeds with various properties, has been shown to have anti-platelet and anti-thrombotic effects. It inhibits platelet aggregation and secretion, regulates platelet adhesion and clot retraction, and activates the cAMP-PKA-VASP pathway. Animal experiments demonstrated its potential therapeutic use in treating and preventing platelet-related cardiovascular disorders.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Food Science & Technology
Hyuk-Woo Kwon, Muhammad Irfan, Yuan Yee Lee, Man Hee Rhee, Jung-Hae Shin
Summary: Cardiovascular diseases are a critical threat to our lives, and platelets play an important role in these diseases. This study evaluated the antiplatelet effects of artocarpesin, a compound isolated from Cudrania tricuspidata, and found that artocarpesin inhibits platelet activity and thrombus formation through the regulation of associated signaling molecules, highlighting its potential value in preventing platelet-induced cardiovascular diseases.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Hae Soo Yun, Sylvatrie-Danne Dinzouna-Boutamba, Sanghyun Lee, Zin Moon, Dongmi Kwak, Dong-Il Chung, Yeonchul Hong, Man Hee Rhee, Youn-Kyoung Goo
Summary: The extract of Petasites japonicus shows potential antimalarial effects by inhibiting the proliferation of Plasmodium parasites and improving hematological parameters. These effects may be attributed to the inhibition of platelet activation related to attenuated glycoprotein VI downstream signaling.
Article
Integrative & Complementary Medicine
Yixian Quah, Jireh Chan Yi-Le, Na-Hye Park, Yuan Yee Lee, Eon-Bee Lee, Seung-Hee Jang, Min-Jeong Kim, Man Hee Rhee, Seung-Jin Lee, Seung-Chun Park
Summary: This study demonstrates the therapeutic benefits of Trifolium pratense ethanolic extract (TPEE) in preventing osteoporosis and maintaining the estrogen dependent characteristics of the uterus. The combination of recursive feature elimination with cross-validation (RFECV) and extreme gradient boosting (XGBoost) model can serve as a powerful tool for quick evaluation and diagnosis of postmenopausal osteoporosis.
Article
Plant Sciences
Yixian Quah, Yuan Yee Lee, Seung-Jin Lee, Sung Dae Kim, Man Hee Rhee, Seung-Chun Park
Summary: Hypercoagulability and platelet hyperactivity are common in severe COVID-19 patients. Natural remedies, such as Panax ginseng, are being explored for their potential in treating COVID-19 by targeting platelet activation and the coagulation cascade. Molecular docking analysis and pharmacokinetic calculations suggest that certain bioactive compounds in P. ginseng have favorable interactions with proteins and receptors involved in platelet activation and good pharmacokinetic profiles.
JOURNAL OF GINSENG RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ji Sue Baik, You Na Seo, Young-Choon Lee, Joo Mi Yi, Man Hee Rhee, Moon-Taek Park, Sung Dae Kim
Summary: This study demonstrates that ionizing radiation activates the p38 MAPK-NLRC4-caspase-1 pathway in macrophages, leading to increased IL-1 beta production in response to lipopolysaccharide treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Hyuk-Woo Kwon, Man Hee Rhee, Jung-Hae Shin
Summary: In this study, it was found that the extract from the white-spotted flower chafer, Protaetia brevitarsis, can inhibit platelet aggregation by regulating cyclic nucleotides and signal transduction molecules. This suggests that the extract has strong antiplatelet effects and may be used to treat cardiovascular diseases associated with thrombosis and platelet activation.
PREVENTIVE NUTRITION AND FOOD SCIENCE
(2023)
Article
Microbiology
Chang Uk Chung, Haeseung Lee, Min-Goo Seo, Seung-Hun Lee, Kyoo-Tae Kim, Kaifa Nazim, Jung-Sun Song, Dong Hwa Bae, Man Hee Rhee, Oh-Deog Kwon, Dongmi Kwak
Summary: This study found that the overall infection rate of Theileria in Korean water deer was 8.1%, with 100% infection rates in the northern and southern regions. The central region had the lowest infection rate. Infection rates also varied depending on the season, with the highest rate observed in spring. Phylogenetic analysis revealed the presence of Theileria luwenshuni in the PCR-positive samples.
Article
Biochemistry & Molecular Biology
Hyuk-Woo Kwon, Sung Dae Kim, Man Hee Rhee, Jung-Hae Shin
Summary: Platelets play a significant role in hemostasis but can also contribute to the development of cardiovascular diseases. Controlling platelet function is important in preventing thrombosis. A compound called 5-hydroxyindolin-2-one (5-HI) has been found to inhibit human platelet aggregation without cytotoxicity and suppress intracellular Ca2+ concentration. It also regulates various pathways to inhibit thromboxane A(2) production and alpha IIb beta 3 action, suggesting its potential as a substance for preventing thrombosis-mediated thrombosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shin-Sook Yoon, Hyuk-Woo Kwon, Jung-Hae Shin, Man Hee Rhee, Chang-Eun Park, Dong-Ha Lee
Summary: This study investigates the effects and mechanisms of artesunate on platelet aggregation and thrombus formation. Results show that artesunate significantly increases cAMP and cGMP production, inhibits calcium ion mobilization, and reduces platelet activity and fibrinogen binding. It also decreases the production of TXA(2) and intracellular granular secretion. These findings suggest that artesunate has potential as an antiplatelet substance to inhibit platelet aggregation and thrombus formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)