期刊
FISH & SHELLFISH IMMUNOLOGY
卷 33, 期 4, 页码 835-845出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2012.07.016
关键词
Amphioxus; Gram-negative bacteria-binding protein (GNBP); Innate immunity; WSC domain; Glycosyl hydrolase family 16; (Glyco_hydro_16 GH16)
资金
- National Natural Science Foundation of China [30970348]
- Project of New Century Excellent Talents in Universities [NCET-07-0405]
- Major Program of Natural Science Research of Jiangsu Higher Education Institutions [12KJA180005]
- Ph.D. Programs Foundation of Ministry of Education of China [20113207110009]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Gram-negative bacteria-binding proteins (GNBPs) are important pattern recognition proteins (PRPs), which can initiate host defense in response to pathogen surface molecules. The roles of GNBP in innate immunity of arthropods and molluscs have recently been reported. However, the GNBP gene has not been characterized in the species of higher evolutionary status yet. In this study, we identified and characterized an amphioxus GNBP gene (designated as AmphiGNBP). First, we identified and cloned the AmphiGNBP and found that the AmphiGNBP encodes a putative protein with 558 amino acids, which contains a conserved beta-1, 3-glucan recognizing and binding domain. Second, we found that the AmphiGNBP encodes two extra WSC (cell Wall integrity and Stress response Component) domains, which are unique in AmphiGNBP protein. The two WSC domains of AmphiGNBP protein coupled with the expansion of amphioxus immunity repertoire might undergo intensive domain shuffling during the age of the Cambrian explosion. Finally, we found that the AmphiGNBP was mainly expressed in immune tissues, such as hepatic cecum and intestine, and the expression of AmphiGNBP was affected after LPS stimulation. In conclusion, our findings disclose the particularity and universality of AmphiGNBP and provide profound insights into the function and evolution of GNBP. (C) 2012 Elsevier Ltd. All rights reserved.
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