期刊
FISH & SHELLFISH IMMUNOLOGY
卷 33, 期 3, 页码 590-596出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2012.06.031
关键词
Channa punctatus; Ficus benghalensis; Antibacterial activity; Phagocytosis; Immunoglobulin
资金
- University Grants Commission (UGC)
- India
- Council of Scientific and Industrial Research (CSIR)
Methanol extract from the dried aerial root of Fiats benghalensis, was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli, by disc diffusion method. In order to study, if there is any immunostimulatory response of E benghalensis, immunized fish were fed with supplementary artificial feed containing 5% E benghalensis dried root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA. (C) 2012 Elsevier Ltd. All rights reserved.
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