Article
Fisheries
Ying Huang, Ying Jiang, Miao-Miao Wang, Min-Yi Chen, Huan-Gen Chen, Heng-Yuan Chen, Wen-Jie Liu, Xiao-Rui Li, Xiao-Lei Han
Summary: This study cloned and characterized a mannose-binding lectin (MBL) called PcMBL from the crayfish Procambarus clarkii. PcMBL plays important roles in nonself recognition and clearance of invading microorganisms. It was found that PcMBL can influence the transcription of antimicrobial peptide genes and has the ability to bind to various microorganisms and carbohydrates. Furthermore, recombinant PcMBL can facilitate bacterial clearance, inhibit biofilm formation and growth, and reduce mortality in infected crayfish.
AQUACULTURE REPORTS
(2023)
Article
Infectious Diseases
Diletta Punginelli, Valentina Catania, Mirella Vazzana, Manuela Mauro, Angelo Spinello, Giampaolo Barone, Giuseppe Barberi, Calogero Fiorica, Maria Vitale, Vincenzo Cunsolo, Rosaria Saletti, Antonella Di Francesco, Vincenzo Arizza, Domenico Schillaci
Summary: The defense system of freshwater crayfish Procambarus clarkii is a diversified source of bioactive molecules with antimicrobial properties, with interesting peptide fractions showing activity against a range of pathogens.
Article
Fisheries
Long Huang, Yu Liu, Xinxin Zhang, Jie Xu, Lu Dai, Lishang Dai, Lehao Huang
Summary: In this study, the cDNA of PcPrx-1 was cloned from Procambarus clarkii and its role in immune system functions against microbial pathogens was investigated. It was found that PcPrx-1 was ubiquitously expressed in all tissues, with highest expression in the hepatopancreas. The gene transcripts of PcPrx-1 were significantly upregulated after exposure to LPS, PGN, and Poly I:C, but the transcription patterns varied after pathogen challenge. Knockdown of PcPrx-1 resulted in a dramatic change in the expression of immune-associated genes in P. clarkii.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Qiu-Ning Liu, Ying-Yu Tang, Yue-Tian Li, Xiao-Han Zha, Ting-Ting Yang, Dai-Zhen Zhang, Jia-Lian Wang, Sen-Hao Jiang, Chun-Lin Zhou, Bo-Ping Tang, Li-Shang Dai
Summary: The study used RNA sequencing to examine the immune response of Procambarus clarkii to lipopolysaccharide (LPS), revealing upregulation of genes related to immune pathways after LPS challenge. Real-time quantitative reverse transcription PCR further validated the expression changes of these genes.
Article
Fisheries
Qing-Ri Jin, Jing-Wei Mao, Fei Zhu
Summary: Anoectochilus roxburghii polysaccharides (ARPs) have significant effects on the immune system of crayfish, increasing the survival rate of crayfish infected with white spot syndrome virus (WSSV). ARPs can also regulate the expression levels of multiple immune genes, promote immune enzyme activity, and induce apoptosis in crayfish hemocytes.
Article
Biotechnology & Applied Microbiology
K. Archana, Sephy Rose Sebastian, K. S. Sruthy, Aishwarya Nair, M. Anju, I. S. Bright Singh, Rosamma Philip
Summary: A novel isoform of anti-lipopolysaccharide factor (Mm-ALF) from the speckled shrimp was characterized, showing high similarity to ALF from another shrimp species. The mature peptide exhibited potent antibacterial activity against both Gram-positive and Gram-negative bacteria, making it a promising antimicrobial agent.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Fisheries
Defang Chen, Lu Lu, Qiaolin Pei, Daiyu Chen, Lei Yang, Xin Zhang, Xiaoli Zhang, Ping Ouyang, Yi Geng, Zhiqiong Li, Liangyu Li, Xiaoli Huang
Summary: This study analyzed the immunological effects of Salvia miltiorrhiza polysaccharides (SMP) on the hemocytes of Procambarus clarkii. The results showed that SMP treatment upregulated the activity of total superoxide dismutase (T-SOD), phenoloxidase (PO), and respiratory burst. Differential gene expression analysis revealed that SMP affected crustacean cellular immune-related signaling pathways. Furthermore, diets supplemented with SMP improved the cellular and humoral immunity of crayfish. This study provides molecular evidence for the immunopotentiator effects of plant polysaccharides in crustaceans.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
Zhengrong Wen, Xiaoli Xu, Dan Xiang, Junfeng Xu, Qiufeng Yang, Xiaofu Wang, Jiashou Liu, Mingzhong Luo, Wei Wei
Summary: This study found that bacterial lipopolysaccharide (LPS) and deoxynivalenol (DON) in the aquatic environment have synergistic toxic effects on red swamp crayfish. Co-exposure to LPS and DON reduces the survival rate and causes histopathological changes in the hepatopancreas. These findings have implications for the development of crayfish aquaculture industry in China.
Article
Microbiology
Zixian Huang, Yuanyuan Li, Chang Cai, Ning Dong
Summary: Red swamp crayfish has become a popular aquatic product in China. The use of antibiotics in modern farming may affect the bacteria in crayfish intestines. This study identified various types of bacteria in crayfish intestines and tested their resistance to antibiotics. It was discovered that most bacteria from crayfish were susceptible to antibiotics, except for one strain of Escherichia coli that carried a last-line antibiotic resistance gene.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Md Muzammel Hossain, Hengliang Huang, Yuan Yuan, Tianyin Wan, Chengfeng Jiang, Zhihua Dai, Shuanglian Xiong, Menghua Cao, Shuxin Tu
Summary: Organosilicon can cause stress response in aquatic animals, affecting the survival rate, antioxidant response, and gene expression of crayfish. It also has an impact on the muscle tissue of crayfish.
ENVIRONMENTAL POLLUTION
(2021)
Review
Engineering, Environmental
Showkat Ali Ganie, Luqman Jameel Rather, Qing Li
Summary: Polysaccharides are natural polymers formed by microorganisms, animals, and plants, playing an important role in the development of living organisms. Curdlan, a natural polymer with exceptional pharmacological characteristics, has the ability to form structures with bioactive molecules for anticancer and antimicrobial applications. It has been explored as a potential biomaterial for delivering drugs with bioactive molecules into biomacromolecules of desired functions.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Plant Sciences
Meenakshi Bhardwaj, Sugumar Mani, R. Malarvizhi, Veeresh Kumar Sali, Hannah R. Vasanthi
Summary: The study showed that a sulfated polysaccharide extracted from marine algae has significant immunomodulatory effects in experimental rats, reducing systemic inflammation and possessing antioxidant and anti-inflammatory potential.
Article
Chemistry, Medicinal
Anguo Li, Ruihao Huang, Chaogang Wang, Qunju Hu, Hui Li, Xiao Li
Summary: In this study, the antimicrobial peptide ALFPm3 from Penaeus monodon with super activity was successfully expressed in Chlamydomonas reinhardtii, leading to the development of a microalga strain capable of producing antimicrobial peptides. The transgenic C. reinhardtii strain showed significant inhibition of bacterial growth, lasting longer than 24 hours, and outperformed ampicillin, indicating its potential as a cell factory for antimicrobial peptide production.
Article
Biochemistry & Molecular Biology
Jie Gong, Mengru Zhu, Ming Zhan, Changjun Xi, Zenghong Xu, Yan Shui, Huaishun Shen
Summary: In this study, the role of the VDAC gene in Procambarus clarkii infected with white spot syndrome virus (WSSV) was investigated. The results suggest that PcVDAC may promote WSSV replication by inhibiting haemocytes apoptosis, providing new insights into the immune response of P. clarkii infected with WSSV.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Immunology
Xiaoling Dai, Mengling Sun, Ximei Nie, Yuqi Zhao, Hao Xu, Zhengxiao Han, Tianheng Gao, Xin Huang, Qian Ren
Summary: Gene duplication (GD) leads to the expansion of gene families, contributing to organism adaptation and defense against pathogens. This study identified two CTL genes (PcLec-EPS and PcLec-QPS) in crustaceans originating from GD. These CTLs can bind to carbohydrates and microbes, activate the Relish transcription factor, and upregulate the expression of antimicrobial peptides (AMPs). Furthermore, there is a positive feedback loop between these CTLs and Relish, regulating the immune response and eliminating pathogenic microorganisms efficiently.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Xin-Le Kang, Jun-Ying Zhang, Di Wang, Yu-Meng Zhao, Xiao-Lin Han, Jin-Xing Wang, Xiao-Fan Zhao
Article
Fisheries
Li-Jie Huo, Ming-Chong Yang, Jin-Xing Wang, Xiu-Zhen Shi
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Cell Biology
Yu-Qin Di, Xiao-Lin Han, Xin-Le Kang, Di Wang, Cai-Hua Chen, Jin-Xing Wang, Xiao-Fan Zhao
Summary: CTSD has dual functions in promoting cell proliferation and apoptosis, with its secretion determined by N233 glycosylation and expression promoted by 20E hormone. Autophagy triggers its maturation and localization inside cells, leading to activation of CASP3 and apoptosis. Its differential expression in tissues plays a role in determining cell fates during lepidopteran metamorphosis.
Review
Fisheries
Zhe Lin, Jia-Lin Wang, Yang Cheng, Jin-Xing Wang, Zhen Zou
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Editorial Material
Fisheries
Jin-Xing Wang, Gerardo R. Vasta
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Review
Fisheries
Xian-Wei Wang, Gerardo R. Vasta, Jin-Xing Wang
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Article
Developmental Biology
Xin-Le Kang, Yan-Xue Li, Yan-Li Li, Jin-Xing Wang, Xiao-Fan Zhao
Summary: Animal steroid hormones can initiate signaling through G protein-coupled receptors. A newly discovered ecdysone-responsive GPCR, ErGPCR-3, plays a crucial role in the lepidopteran insect Helicoverpa armigera. Under 20E induction, ErGPCR-3 forms a homotetramer to facilitate 20E entry into cells, transmitting the 20E signal and promoting metamorphosis.
Article
Immunology
Cang Li, Ming-Chong Yang, Pan-Pan Hong, Xiao-Fan Zhao, Jin-Xing Wang
Summary: The study revealed that after infection with White spot syndrome virus (WSSV), the content of linoleic acid significantly increased in shrimp, suggesting its involvement in the shrimp's antiviral response. Further experiments demonstrated that oral administration of linoleic acid reduced WSSV proliferation in shrimp and increased shrimp survival rate.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Microbiology
Cang Li, Pan-Pan Hong, Ming-Chong Yang, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study identified the involvement of FOXO in systemic and cellular immunity regulation in invertebrates, specifically in shrimp. FOXO plays a critical role in maintaining hemolymph and intestinal microbiota homeostasis by promoting the expression of antimicrobial peptides and enhancing phagocytosis of hemocytes against pathogens. The study revealed the different functions of FOXO in mucosal and systemic antibacterial immunity of invertebrates, providing insights for disease prevention and control in aquaculture.
Article
Genetics & Heredity
Xiao-Pei Wang, Zhen Huang, Yan-Li Li, Ke-Yan Jin, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao
Summary: The regulation of glycometabolism homeostasis is crucial for maintaining health and development. This study used insects as a model and found that the steroid hormone 20-hydroxyecdysone (20E) upregulated the expression of the transcription factor Klf15 to promote autophagy, apoptosis, and gluconeogenesis, thus maintaining glucose homeostasis.
Article
Virology
Peng-Yuan Lu, Guo-Juan Niu, Pan-Pan Hong, Jin-Xing Wang
Summary: In shrimp, the LOXL protein plays a crucial role in antiviral and antibacterial innate immunity by enhancing the expression of antimicrobial peptides.
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: This study reveals the crucial role of the mTORC1 signaling pathway in the replication of white spot syndrome virus (WSSV) in Marsupenaeus japonicus. WSSV activates calmodulin, leading to the activation of mTORC1 and its downstream effector S6Ks for viral protein translation. This finding suggests that mTORC1 may serve as a potential therapeutic target for controlling WSSV in shrimp aquaculture.
Article
Multidisciplinary Sciences
Xiu-Zhen Shi, Ming-Chong Yang, Xin-Le Kang, Yan-Xue Li, Pan-Pan Hong, Xiao-Fan Zhao, Gerardo R. Vasta, Jin-Xing Wang
Summary: The activation of the IMD pathway, critical for host immunity, is mediated by the receptor SRB2 in crustaceans. Depletion of SRB2 leads to increased bacterial proliferation, shrimp mortality, and decreased expression of antimicrobial peptides. This previously unrecognized SRB2-IMD axis provides insight into the innate immune response in crustaceans and has implications for prevention and treatment strategies against shrimp diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Peng Zhang, Hai-Jing Fu, Li-Xia Lv, Chen-Fei Liu, Chang Han, Xiao-Fan Zhao, Jin-Xing Wang
Summary: AMPK is found to play a significant role in the response of shrimp to white spot syndrome virus (WSSV). The study demonstrates that WSSV infection leads to upregulation of AMPK expression and phosphorylation. The findings suggest that WSSV exploits the host CaMKK-AMPK-mTORC2 pathway for its proliferation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
Summary: Previous studies have shown that the mTORC1 signaling pathway plays a role in antiviral functions or benefits viral replication, but the detailed mechanisms by which mTORC1 enhances viral infection are unclear. Our study reveals that WSSV utilizes the mTORC1 pathway for replication in shrimp, suggesting a potential clinical target for controlling WSSV in shrimp aquaculture.