Article
Immunology
Xu Zhou, Gui-Rong Zhang, Wei Ji, Ze-Chao Shi, Xu-Fa Ma, Zun-Lan Luo, Kai-Jian Wei
Summary: The study revealed that the immune response of yellow catfish against E. ictaluri infection is mainly manifested in the spleen and liver, with key gene expression peaking at 72 hours post-infection. Cytokines were continuously transcribed in the spleen, while the liver mainly secreted components of the complement system, and metabolic regulation was activated from 12 to 120 hours post-infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Tan-Trung Nguyen, Tran T. T. Xuan, To H. Ngoc, Le T. My Duyen, Tu Q. Vinh, Pham D. T. My, Hoang A. Hoang, Le P. Nga
Summary: This study reveals the existence of two novel bacteriophages infecting Edwardsiella ictaluri, vB_EiM_PVN06 and vB_EiA_PVN09, originating from the Mekong Delta in Vietnam. These bacteriophages exhibit distinct biological properties and show potential for phage therapy in the control of ESC disease.
Article
Fisheries
Adrian Lopez-Porras, Matt J. Griffin, Cynthia Ware, Bradley M. Richardson, Terrence E. Greenway, Thomas Graham Rosser, Suja Aarattuthodiyi, David J. Wise
Summary: Edwardsiella piscicida is a growing problem in catfish aquaculture in the southeastern United States, especially in channel and hybrid catfish. Multiple phyletic groups of E. piscicida have been identified, with no significant differences in virulence between channel and hybrid catfish. A live-attenuated E. ictaluri vaccine has shown cross-protection against one E. piscicida isolate, but its effectiveness against other variants is unknown. Exposure to heterologous E. piscicida isolates improves survival following E. ictaluri challenge, suggesting the presence of shared and conserved antigens.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Nermeen Y. Abass, Rhoda Mae C. Simora, Jinhai Wang, Shangjia Li, De Xing, Michael Coogan, Andrew Johnson, David Creamer, Xu Wang, Rex A. Dunham
Summary: This study demonstrates that genetic enhancement of the channel catfish's resistance to Edwardsiella ictaluri can be achieved through cecropin transgenesis. The transgenic fish exhibited enhanced disease resistance and improved survival time when challenged with the bacteria. The growth rates of transgenic and non-transgenic fish were not different, indicating that the transgene did not affect the overall growth of the fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Nguyen Van Sang, Tran Thi Phuong Dung, Vo Hong Phuong, Nguyen Hong Nguyen, Nguyen Huu Thinh
Summary: The present study analyzed the immune responses of resistant (RF) and susceptible families (SF) of striped catfish (Pangasianodon hypophthalmus) to Edwardsiella ictaluri, the causative agent of bacillary necrosis of Pangasius. The RF group showed a more effective non-specific immune response compared to the SF group. White blood cell counts increased significantly in the RF group, starting from 48 hours post infection (hpi), while the increase was slower in the SF group, mainly after 264 hpi. Monocyte and neutrophil development was faster in the RF families, and the number of melano-macrophage centers was higher in the RF group at 24-48 hpi. The RF group also exhibited a higher specific immune response (antibody titer) compared to the SF group. The study identified the critical period of 24-48 hpi for differentiating disease resistant individuals, with an Area Under the Curve (AUC) value of 0.95.
Article
Immunology
Ying Li Liu, Tian Tian He, Xiu Long Jiang, Shan Shan Sun, Long Kun Wang, Pin Nie, Hai Xia Xie
Summary: An attenuated E. ictaluri strain was developed as a vaccine candidate by deleting the T3SS protein eseJ(ei) gene. The study found that this vaccine candidate is easily administered via bath immersion and highly effective in stimulating protection against E. ictaluri infection, resulting in a significantly higher survival rate.
MICROBIAL PATHOGENESIS
(2022)
Article
Biochemistry & Molecular Biology
Shen-Li You, Xin-Xin Jiang, Gui-Rong Zhang, Wei Ji, Xu-Fa Ma, Xu Zhou, Kai-Jian Wei
Summary: This study cloned and identified the open reading frame sequences of TRAF genes in yellow catfish and found that these genes play important roles in immune response and may be involved in the immune response against bacterial infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Ahmed Abdelmoniem Mousa, Reshma Ramachandran, Ozan Ozdemir, Attila Karsi, Hossam Abdelhamed
Summary: The study showed that dietary trans-cinnamaldehyde (TC) can increase the activity of superoxide dismutase (SOD) and decrease the concentration of malondialdehyde (MDA) in hepatic tissue of channel catfish. Additionally, after infection with Edwardsiella ictaluri, catfish fed with TC demonstrated significantly increased antioxidant defense responses on days 1, 6, and 14 post-infection. However, there were no significant differences in the activities/concentrations of catalase (CAT), reduced glutathione (GSH), nitric oxide (NO), and MDA between the TC and control groups.
Article
Fisheries
Haoxuan Li, Liqiang Zhang, Yunzhen Yu, Taoshan Ai, Yongan Zhang, Jianguo Su
Summary: Yellow catfish is an important freshwater cultured fish in China, but it is susceptible to enteric septicemia and red-head disease caused by Edwardsiella ictaluri, resulting in significant economic losses in the farming industry. This study developed rapid and accurate detection methods, including RPA, real-time RPA, and RPA-LFD, targeting a specific gene sequence in E. ictaluri. These methods demonstrated high sensitivity and specificity, and showed great potential for diagnosing E. ictaluri disease in fish farms.
Article
Infectious Diseases
Ning Xu, Miao Li, Xiaohui Ai, Zhoumeng Lin
Summary: The study found that administering DC at a dose of 20 mg/kg every 96 hours is a preferable regimen in yellow catfish, demonstrating good pharmacokinetic-pharmacodynamic effects against Edwardsiella ictaluri.
Article
Microbiology
Allison L. Wise, Benjamin R. LaFrentz, Anita M. Kelly, Mark R. Liles, Matt J. Griffin, Benjamin H. Beck, Timothy J. Bruce
Summary: A preliminary assessment was conducted to study the bacterial coinfection of Edwardsiella ictaluri and Flavobacterium covae in juvenile channel catfish. The coinfection challenges showed similar cumulative percent mortality (CPM) to the single dose E. ictaluri challenge, but the onset of peak mortality was delayed in fish exposed to F. covae first. Additionally, catfish exposed to E. ictaluri displayed increased serum lysozyme activity and higher expression of pro-inflammatory cytokines.
Article
Microbiology
Huihui Han, Da Teng, Ruoyu Mao, Ya Hao, Na Yang, Zhenlong Wang, Ting Li, Xiumin Wang, Jianhua Wang
Summary: In this study, four derivatives of marine peptide-N6NH2 were designed and GUON6NH2 showed superior stability, lower toxicity, longer post-antibiotic effect, higher intracellular inhibition rate against Edwardsiella tarda, suggesting it as a potential novel antimicrobial agent.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Jicheng Yang, Yaoyao Lin, Zhaohui Wei, Zhenbing Wu, Qianqian Zhang, Jingwen Hao, Shuyi Wang, Aihua Li
Summary: 16S rRNA gene high-throughput sequencing technology was used to investigate the changes in the gut microbiota of yellow catfish infected by Edwardsiella ictaluri. The microbial composition and diversity of the diseased fish were significantly different from those of healthy fish. Proteobacteria became the dominant phylum in the gut of the diseased fish, while Cetobacterium, Plesiomonas, and Romboutsia disappeared. The gut microbiota of the diseased fish showed reduced anaerobic and Gram-positive bacteria, as well as decreased digestive system, immune system, and metabolic functions.
Article
Biotechnology & Applied Microbiology
Vimbai Irene Machimbirike, Pichahpuk Uthaipaisanwong, Pongsak Khunrae, Ha Thanh Dong, Saengchan Senapin, Triwit Rattanarojpong, Sawannee Sutheeworapong
Summary: E. ictaluri has been recognized as a major threat to catfish aquaculture for over 40 years, with only 9 publicly available sequenced genomes. Our study reports two new complete genomes of E. ictaluri isolated from diseased hybrid red tilapia and striped catfish in Southeast Asia. Through core genome MLST (cgMLST) and ANI values, E. ictaluri isolates were consistently grouped into 4 host-specific genotypes, offering potential biomarkers for further genotyping scheme development. High antigenic, solubility, and secretion probabilities of vaccine candidates were identified in silico from the core genes.
Article
Fisheries
Uni Purwaningsih, Sukenda Sukenda, Angela M. Lusiastuti, Alimuddin Alimuddin, Widanarni Widanarni, Sri Nuryati
Summary: This study compared the phenotypic, genotypic characteristics, and pathogenicity of Edwardsiella ictaluri from different catfish farms in Indonesia. The results showed variations in growth patterns, enzyme activity, antibiotic resistance, and genetic profiles among the isolates. The protein components of E. ictaluri cells were found to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.
AQUACULTURE RESEARCH
(2022)