Article
Fisheries
Nora Albaladejo-Riad, Alberto Cuesta, M. Angeles Esteban
Summary: The aim of this study was to induce and characterize extracellular traps (ETs) produced by gilthead seabream (Sparus aurata L.) head-kidney leucocytes. After stimulating the leucocytes with various substances, ETs structures were observed and quantified, revealing different morphological properties depending on the stimulant used. This research provides important insights into the immune response of fish and its potential application in mitigating microbial infections in fish farms.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jose Carlos A. Campos-Sanchez, Francisco A. Guardiola, Mara Angeles Esteban
Summary: This study investigated the effects of cantharidin on the inflammatory response in gilthead seabream head-kidney leucocytes (HKLs). The results showed that cantharidin induced apoptosis in HKLs, decreased respiratory burst and phagocytic activities, and increased peroxidase activity. Furthermore, cantharidin also affected the expression of different genes in HKLs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Jose Carlos Campos-Sanchez, Francisco A. Guardiola, Maria Angeles Esteban
Summary: This study investigated the effects of lambda-carrageenin on gilthead seabream head-kidney leucocytes (HKLs). The results showed that lambda-carrageenin had no negative effects on cell viability but increased respiratory burst activity and phagocytic ability of HKLs. It also affected the expression of inflammation-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Joana P. Firmino, Laura Fernandez-Alacid, Eva Vallejos-Vidal, Ricardo Salomon, Ignasi Sanahuja, Lluis Tort, Antoni Ibarz, Felipe E. Reyes-Lopez, Enric Gisbert
Summary: Dietary supplementation of garlic essential oil, carvacrol, and thymol in gilthead seabream juveniles modulated skin transcriptional immune response, bacterial growth capacity in skin mucus, and overall health condition. The supplementation promoted innate immunity and mucus protective capacity, reducing susceptibility to pathogenic bacteria colonization.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Medicinal
Bruno Reis, Lourenco Ramos-Pinto, Sara A. Cunha, Manuela Pintado, Joana Laranjeira da Silva, Jorge Dias, Luis Conceicao, Elisabete Matos, Benjamin Costas
Summary: This study evaluated the effects of different feed formulations containing Chlorella vulgaris on the health status and inflammatory response of gilthead seabream. The results showed that Chlorella vulgaris supplementation had an impact on the circulating neutrophils in the fish, but had mild effects on the innate immune and antioxidant functions. In the inflammatory response, the soluble peptide-enriched extract of Chlorella vulgaris seemed to have a protective, anti-stress effect in the gut.
Article
Immunology
Ricardo Salomon, Felipe E. Reyes-Lopez, Lluis Tort, Joana P. Firmino, Carmen Sarasquete, Juan B. Ortiz-Delgado, Jose C. Quintela, Jose M. Pinilla-Rosas, Eva Vallejos-Vidal, Enric Gisbert
Summary: The study showed that the inclusion of medicinal plant leaf extract from sage and lemon verbena in the diet of gilthead seabream had beneficial effects on gut health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Lourenco Ramos-Pinto, Marina Machado, Josep Calduch-Giner, Jaume Perez-Sanchez, Jorge Dias, Luis E. C. Conceicao, Tome S. Silva, Benjamin Costas
Summary: The study aimed to evaluate the effects of histidine, taurine, and threonine supplementation on the immune status of gilthead seabream. The results showed that dietary supplementation with these amino acids had mild immune-modulation effects on gilthead seabream, particularly noteworthy for further study under disease challenge conditions.
Article
Immunology
Rocio Leiva-Rebollo, Juan Gemez-Mata, Dolores Castro, Juan J. Borrego, Alejandro M. Labella
Summary: The DNA vaccine based on the major capsid protein of Lymphocystis disease virus 3 has been found to induce changes in the expression of immune-related genes in vaccinated gilthead seabream. The vaccine can effectively decrease viral replication and modulate the inflammatory and interferon response, leading to control of the infection progression.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Dimitra Kogiannou, Chrysanthi Nikoloudaki, Vasilia Valsamidi, Mado Kotsiri, Eleni Golomazou, George Rigos
Summary: The pharmacokinetics of orally administered praziquantel (PZQ) were studied in gilthead seabream, with rapid absorption and higher drug levels observed in both plasma and gills with the high dose regimen. However, the elimination rate of PZQ from fish tissues was fast.
Article
Biotechnology & Applied Microbiology
B. Reis, L. Ramos-Pinto, J. A. Martos-Sitcha, M. Machado, R. Azeredo, S. Fernandez-Boo, S. Engrola, C. Unamunzaga, J. Calduch-Giner, L. E. C. Conceicao, T. Silva, J. Dias, B. Costas, J. Perez-Sanchez
Summary: This study evaluated the effects of incorporating Phaeodactylum tricornutum in fish feed on fish health and performance. Results indicate that the BC diet may have a potential stimulatory effect on enhancing immune response.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Environmental Sciences
Igor Talijancic, Iva Zuzul, Viktorija Kiridzija, Jasna Siljic, Jelka Pleadin, Leon Grubisic, Tanja Segvic-Bubic
Summary: Fish farms have a high attractivity to wild fish, and the influence of tuna farming on plastic traits of gilthead seabream remains largely unknown. The study found significant differences in habitat selection and foraging behavior between farm-associated seabream and wild or farmed seabream, highlighting the existence of resource polymorphism in gilthead seabream.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
Nora Albaladejo-Riad, Cristobal Espinosa-Ruiz, Maria Angeles Esteban, Carlo C. Lazado
Summary: Molecular processes and metabolomic insights of skin wound healing in gilthead seabream were investigated. The skin mucus metabolome of wounded and non-wounded seabream fed with silk fibroin microparticles was analyzed. The study revealed that dietary manipulation had a more profound effect on skin mucus than wound, and supplementation of silk fibroin increased methionine level in skin mucus, which correlated with wound healing capacity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Antonia M. Jimenez-Monreal, Francisco A. Guardiola, M. Angeles Esteban, M. Antonia Murcia Tomas, Magdalena Martinez-Tome
Summary: This study investigated the effect of dietary supplementation with Moringa leaves on the antioxidant activity of gilthead seabream, concluding that higher percentages of Moringa led to increased antioxidant activity in the fish. The antioxidant potential of Moringa leaves was mainly attributed to the presence of polyphenolic compounds.
Article
Microbiology
Alberto Ruiz, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep a. Calduch-Giner, Marc Vinas, Jaume Perez-Sanchez, Enric Gisbert
Summary: Given the potential role of bile salts in lipid digestion, this study evaluated the effects of bile salts supplementation on gut immune response and microbial populations in gilthead seabream. The results showed differential modulation of the microbiota in the anterior and posterior intestine, as well as changes in gene expression related to intestinal health and immune response. Overall, bile salts supplementation could enhance fish farming without compromising intestinal health.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Alexandre Mignucci, Jerome Bourjea, Fabien Forget, Hossein Allal, Gilbert Dutto, Eric Gasset, David J. McKenzie
Summary: The study found that cardiac responses of gilthead seabream to hypoxia and warming differed between swimming freely in a tank and being confined to individual respirometers, with spontaneous activity contributing to heart rate differences. Additionally, there was a significant linear relationship between heart rate and oxygen uptake rates, suggesting that respirometry techniques may bias metabolic trait estimates in some fishes.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Fisheries
Jose Carlos Campos-Sanchez, Enrica Vitarelli, Francisco A. Guardiola, Diana Ceballos-Francisco, Jose Maria Garcia Beltran, Antonio Ieni, Maria Angeles Esteban
Summary: This study evaluated local inflammation mechanisms in the skin of gilthead seabream induced by carrageenin, finding an increase in skin mucus-secreting cells and acidophilic granulocytes after injection. Gene expression of various markers and proinflammatory genes was also up-regulated post-injection. Results indicate the chronological involvement of important immune cells in resolving inflammation in the fish skin.
JOURNAL OF FISH DISEASES
(2022)
Article
Chemistry, Physical
Gurjaspreet Singh, Mohit, Diksha, Suman, Priyanka, K. N. Singh, D. Gonzalez-Silvera, Cristobal Espinosa-Ruiz, Maria Angeles Esteban
Summary: This study proposed the synthesis of chalcone derived organosilanes based on polycyclic aromatic compounds, and categorized the synthesized compounds using various spectroscopic techniques. The compound showed high affinity towards various metal ions, particularly Sn2+, and demonstrated improved detection capabilities for Sn(II) ions when bound to Fe-based magnetic silica nanoparticles.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Adriana Trapani, Maria Angeles Esteban, Francesca Curci, Daniela Erminia Manno, Antonio Serra, Giuseppe Fracchiolla, Cristobal Espinosa-Ruiz, Stefano Castellani, Massimo Conese
Summary: This study investigates the use of solid lipid nanoparticles (SLN) to deliver a mixture of antioxidant compounds to fish cells in aquaculture. The findings show that an SLN formulation containing Gelucire(R) 50/13 and Precirol(R) ATO5 can improve the viability and antioxidant properties of fish cells. This research may have significant implications in the field of aquaculture.
Article
Environmental Sciences
Nicole Francesca Pelusio, Alessio Bonaldo, Enric Gisbert, Karl B. Andree, Maria Angeles Esteban, Francesco Dondi, Maria Chiara Sabetti, Pier Paolo Gatta, Luca Parma
Summary: The study aimed to provide practical feeding management guidelines for stressful farming practices. The results suggest that 10% FM and 3% FO dietary levels might affect stress and immune responses.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemical Research Methods
Marta Pastor-Belda, Natalia Arroyo-Manzanares, Natalia Campillo, Pilar Vinas, Crystal Guluarte, Carlos Angulo, Maria Angeles Esteban
Summary: This study used ultra-high-performance liquid chromatography with mass spectrometry to identify bioactive compounds from different yeast species. The results showed that these yeasts are a good source of nucleobases, nucleosides, and nucleotides, which have potential as immunostimulants for food additives.
Article
Fisheries
Maria M. Camara-Ruiz, Jose Maria Garcia-Beltran, Isabel M. Cerezo, M. Carmen Balebona, Miguel Angel Morinigo, Maria Angeles Esteban
Summary: Fish stress is a major concern in aquaculture industry. This study found that air exposure stress caused changes in skin mucosal immunity and microbial composition in gilthead seabream, which may be beneficial for the fish to adapt to stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Nora Albaladejo-Riad, Cristobal Espinosa Ruiz, M. Angeles Esteban
Summary: The impact of dietary supplementation with silk fibroin microparticles on wound healing process in gilthead seabream was studied. Results showed that the SF100 diet improved wound closure ratio, enhanced skin cell proliferation, and increased the expression of important genes involved in tissue repair. This is important for wound healing in gilthead seabream.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Laura Cervera, Carmen Gonzalez-Fernandez, Daniela Cano, M. Angeles Esteban, Luis Mercado, Elena Chaves-Pozo, Alberto Cuesta
Summary: Antimicrobial peptides (AMPs) are potent immune system components that can kill pathogens and modulate the immune response. This study found that AMPs in European sea bass can enhance immune response and antibacterial activity but do not improve resistance to nodavirus (NNV) disease.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yulema Valero, Luis Mercado, Marta Arizcun, Alberto Cuesta, Elena Chaves-Pozo
Summary: Immunity plays a crucial role in the development of fish embryos, and maternal transfer of immunity positively affects the innate immune system development of their progeny. In this study, priming broodstock female fish before spawning season enhances the innate immune activities and upregulates the expression of antimicrobial peptides in their offspring. Furthermore, evidence of maternal transfer of immune proteins related to antimicrobial responses is provided.
Article
Fisheries
Miguel Angel Garcia-Alvarez, Carmen Gonzalez-Fernandez, Maria Angeles Esteban, Alberto Cuesta
Summary: In this study, the cytotoxic and regulatory T cell molecule CRTAM and its ligand CADM1 were identified in European seabass and gilthead seabream. The transcriptional levels of these genes were evaluated and showed constitutive expression, with higher expression in immune tissues. Nodavirus infection induced up-regulation of CRTAM and CADM1, particularly in seabream, which may explain their resistance to nodavirus disease.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Cristobal Espinosa-Ruiz, Carmen Gonzalez-Fernandez, Bettie Cormier, Steffen H. Keiter, Luis R. Vieira, Lucia Guilhermino, Christelle Clerandeau, Jerome Cachot, Maria A. Esteban, Alberto Cuesta
Summary: Marine environments are contaminated with plastic waste, including microplastics, which are ingested by aquatic organisms and have negative effects on animal welfare. The interactions between microplastics, contaminants, and organisms are not well understood. The study on European seabass showed that the effects of microplastics and perfluorooctanesulfonic acid (PFOS) on fish were similar or even lesser when combined, indicating a lack of additive effects or potential protection against PFOS toxicity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Fisheries
Yulema Valero, Alberto Cuesta
Summary: Aquaculture is responsible for over half of the fish consumed by humans worldwide, but infectious diseases caused by viruses are a major problem. The coexistence of different virus strains in the same area allows for natural reassortment, which is a recombination process where different viruses exchange their genetic material. This has been facilitated by the transportation of fish eggs and larvae between facilities globally.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Salma Guebebia, Cristobal Espinosa-Ruiz, Lazhar Zourgui, Alberto Cuesta, Mehrez Romdhane, M. Angeles Esteban
Summary: The study examined the immunostimulant, cytotoxic, bactericidal, and antioxidant activities of ethanolic extracts from different parts of okra. The extracts showed significant content of phenols and flavonoids. They had notable effects on leukocyte activities in European sea bass and demonstrated cytotoxic effects on cell lines. Furthermore, they exhibited bactericidal activity against fish pathogenic bacteria and antioxidant properties. The results suggest the potential use of okra extracts as an alternative to chemical compounds in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Elena Chaves-Pozo, Alberto Cuesta
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Javier Mayor, Alberto Cuesta, Cristobal Espinosa-Ruiz, M. Angeles Esteban
Summary: This study investigated the effects of Astaxanthin (AX) on various fish pathogenic bacteria and fish cell lines. The results showed that AX had different effects on different bacteria and cell lines, providing valuable insights into the mechanism of action of AX in aquaculture.
AQUACULTURE REPORTS
(2023)