Article
Fisheries
Xuangang Wang, Xiangfu Kong, Xiaobing Liu, Xubo Wang, Zhigang Wang, Jinxiang Liu, Quanqi Zhang, Haiyang Yu
Summary: Pyroptosis is a caspase-1-dependent form of programmed cell death characterized by cell lysis and inflammatory cytokine release. This study demonstrates that E. tarda infection triggers pyroptosis in macrophages of Japanese flounder, playing a vital role in the immune response. Treatment with a caspase-1 inhibitor significantly reduces pyroptosis, indicating the association of caspase-1 with pyroptosis.
Article
Fisheries
Ruoxin Wang, Ping Han, Xiumei Liu, Xubo Wang
Summary: In this study, the role of TRAF genes in the immune response of Japanese flounder was investigated. Six TRAF genes were identified and analyzed, and their expression patterns were studied in different developmental stages and tissues. It was found that some of these genes showed differential expression under temperature stress and pathogen challenge, suggesting their involvement in the immune response. These findings provide insights into the molecular roles of TRAF genes in the regulation of immune and inflammatory responses in Japanese flounder.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Hee Jae Choi, Ji-Hoon Lee, Da Yeon Choi, Ji Hee Kim, Jin Man Cho, Hyuk Chu Kwon, Yue Jai Kang
Summary: This study evaluated the effects of betaine-supplemented feed on the growth and immunity of olive flounders. The results showed that betaine can promote growth and enhance immunity against bacterial infections in fish, potentially providing an alternative to conventional antibiotics in aquaculture.
JOURNAL OF FISH DISEASES
(2022)
Article
Genetics & Heredity
Saisai Liu, Haofei Song, Zeyu Liu, Wei Lu, Quanqi Zhang, Jie Cheng
Summary: This study identified suitable reference miRNAs for quantitative expression analysis of miRNAs in Japanese flounder (Paralichthys olivaceus). Through miRNA-Seq analysis and literature search, 10 candidates were selected and their expression stability was evaluated using qRT-PCR and multiple methods. The results showed that miR-22-3p and miR-23a-3p were suitable reference miRNAs for both normal tissues and livers infected with E. tarda in P. olivaceus. These findings provide valuable information for future miRNA expression analysis in P. olivaceus.
Article
Fisheries
Yuanyuan Ding, Shun Zhou, Huimin Guo, Ying Li, Ning Ding, Yunji Xiu
Summary: Galectins are a family of lectins that play crucial roles in the innate immune systems of vertebrates and invertebrates by binding to β-galactoside sugars through their conserved CRDs. This study identified and characterized a PoGRP cDNA from Paralichthys olivaceus, showing it to be widely expressed in various tissues and significantly upregulated in response to bacterial challenge. The recombinant PoGRP protein exhibited binding to various PAMPs and bacteria, suggesting a vital role in protecting against bacterial infection in fish.
Article
Fisheries
Dong-Gyun Kim, Su-Jeong Lee, Jong Min Lee, Eun-Woo Lee, Won Je Jang
Summary: The composition of gut microbiota in flounder changed following infection with pathogenic bacteria. Edwardsiella tarda infection decreased Verrucomicrobia abundance and increased Proteobacteria abundance. Streptococcus iniae infection reduced Verrucomicrobia abundance. Vibrio harveyi infection decreased Firmicutes and Verrucomicrobia abundance and increased Proteobacteria. Infection with all three pathogens increased the relative abundance of Ralstonia and Sphingomonas species and decreased Rubritalea, Saccharimonas, and Bacillus species. Therefore, regulating the gut microbiota composition by reducing Ralstonia and Sphingomonas abundance and increasing Rubritalea, Saccharimonas, and Bacillus abundance might help maintain a healthy gut microbiota balance in flounder. This research could be useful for future studies on improving flounder health through gut microbiota regulation.
Article
Fisheries
Rui Li, Jiangbo Qu, Hengshun Li, Quanqi Zhang
Summary: This study identified 19 scavenger receptor (SRs) family members in Japanese flounder using genome and transcriptome data. Phylogenetic and Ka/Ks analysis revealed five classes within the SRs genes and evidences of purified selection pressures. The study also found that SRs genes are severely conservative within different subfamilies based on gene structure, functional domain, and motif distribution analyses. Additionally, the study demonstrated the important roles of SRs genes in embryogenesis and innate immune response.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Hongxiang Wang, Ming Guo, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The study showed that flounder IFN-gamma could act as a promising adjuvant candidate for fish vaccination, significantly enhancing immune responses and protective effects against E. tarda. Co-vaccination with IFN-gamma could boost specific antibody levels and immune-related gene expressions, leading to higher survival rates in vaccinated fish.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Oceanography
Yan Weijie, Qiao Yingjie, Liu Yuxiang, Liu Xiumei, Zhang Quanqi, Wang Xubo
Summary: Heat shock proteins 10/60 (hsp10/60) in Japanese flounder exhibit evolutionarily conserved characteristics and immune response functions, especially showing significantly regulated expressions after bacterial infections. The study findings provide valuable information for clarifying the evolutionary relationship and immune functions of these genes.
JOURNAL OF OCEAN UNIVERSITY OF CHINA
(2021)
Article
Immunology
Xiaoyan Wu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study demonstrates that the inactivated vaccine against Edwardsiella tarda can induce a protective immune response in flounder, which is mediated through the immediate recognition of the pathogen and subsequent activation of cellular immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xue-Peng Li, Jian Zhang
Summary: This study utilized RNA-Seq technology to investigate the molecular mechanism of fish immune response following TXhfq vaccination, revealing a large number of differentially expressed genes in different tissues, with implications in six immune-related categories. A protein-protein interaction network highlighted the significance of the top six hub genes regulated by TXhfq in immune processes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Infectious Diseases
Yuying Fu, Lishan Zhang, Huanhuan Song, Junyan Liao, Li Lin, Wenjia Jiang, Xiaoyun Wu, Guibin Wang
Summary: This study investigates the role of lysine acetylation and succinylation in the antibiotic resistance mechanism of Edwardsiella tarda. The results demonstrate that these post-translational modifications play important roles in bacterial antibiotic resistance and are enriched in various metabolic pathways. The findings provide insights into the mechanism of antibiotic resistance and may aid future research on the pathogenesis of this bacterium.
Article
Fisheries
Rafaela A. Santos, Nuno Mariz-Ponte, Nicole Martins, Rui Magalhaes, Russell Jerusik, Maria J. Saavedra, Helena Peres, Aires Oliva-Teles, Claudia R. Serra
Summary: The study revealed that extracellular molecules from Bacillus spp. fish isolates improved the in vitro response of gilthead seabream immune cells and are promising candidates as immunostimulants to help fish fight diseases.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Huihui Xie, Jiabao Hu, Yajun Wang, Xubo Wang
Summary: This study conducted a comprehensive analysis of MMPs in Japanese flounder, including gene structures, evolutionary relationships, conserved domains, molecular evolution, and expression patterns. The analysis revealed that MMP genes could be grouped into 17 sub-families and were evolutionarily conserved and functionally-constrained. The expression profiling showed that MMPs had diverse functions in environmental stress, with different expression patterns observed under temperature stress and Edwardsiella tarda infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Jingjing Cao, Kunihiko Futami, Masashi Maita, Teruyuki Nakanishi, Takayuki Katagiri
Summary: This study investigated the immune response and vaccine efficacy in fish vaccinated with different vaccines. The results showed that the allogeneic + FKC group had the best protection effect, achieved through the induction of CMI. The study provides important information for vaccine development.
FISH & SHELLFISH IMMUNOLOGY
(2023)