期刊
FISH & SHELLFISH IMMUNOLOGY
卷 25, 期 6, 页码 834-840出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2008.09.005
关键词
Vibrio splendidus; Vibrio anguillarum; Micrococcus lysodeikticus; Clearance; Flow cytometry; Hyalinocytes; Granulocytes; Mytilus; Molluscs
资金
- Ministero dell'Universita a della Ricerca-Italy
- EU [FOOD-CT-2005-007103]
- Natural Environment Research Council [NER/S/A/2005/13136]
- CNRS
Mussels are filter-feeders living in a bacteria-rich environment. We have previously found that numerous bacterial species are naturally present within the cell-free hemolymph, including several of the Vibrio genus, whereas the intra-cellular content of hemocytes was sterile. When bacteria were injected into the circulation of the mussel, the number of living intra-hemocyte bacteria dramatically increased in less than an hour, suggesting intense phagocytosis, then gradually decreased, with no viable bacteria remaining 12 h post-injection for Micrococcus lysodeikticus, 24 h for Vibrio splendidus and more than 48 h for V ibrio anguillarum. The total hemocyte count (THC) was dramatically lowered by the bacterial injections, as quantified by flow cytometry. V. splendidus induced the strongest decreases with -66% 9 h post-injection of living bacteria and -56% 3 h post-injection of heat-killed bacteria. Flow cytometry was used to identify three main sub-populations of hemocytes, namely hyalinocytes, small granulocytes and large granulocytes. When THC was minimal, i.e. within the first 9 h post-injection, proportions of the three cell categories varied dramatically, suggesting differential involvement according to the targets, but small granulocytes remained the majority. According to a decrease in their number followed by an increase (+90% at 12 h with living V. splendidus), hyalinocytes also appeared to be involved as cellular effectors of antibacterial immunity, despite possessing little capacity for phagocytosis and not containing antimicrobial peptides. (C) 2008 Elsevier Ltd. All rights reserved.
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