期刊
FISH & SHELLFISH IMMUNOLOGY
卷 25, 期 3, 页码 315-320出版社
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2008.04.012
关键词
white spot syndrome virus; WSSV; shrimp Penaeus japonicus; recombinant VP28 protein; oral administration; immunostimulant
资金
- Japan Science and Technology Corporation (JST)
- Creation and Support Program
White spot syndrome virus (WSSV) disease is a major threat to shrimp culture worldwide. Here, we assessed the efficacy of the oral administration of purified recombinant VP28, an envelope protein of WSSV, expressed in a Gram-positive bacterium, Brevibacillus brevis, in providing protection in shrimp, Penaeus japonicus, upon challenge with WSSV. Juvenile shrimp (2-3 g in body weight) fed with pellets containing purified recombinant VP28 (50 mu g/shrimp) for 2 weeks showed significantly higher survival rates than control groups when challenged with the virus at 3 days after the last day of feeding. However, when shrimp were challenged 2 weeks after the last day of feeding, survival rates decreased (33.4% and 24.93%, respectively). Survival rate was dose-dependent, increasing from 60.7 to 80.3% as the dose increased from 1 to 50 mu g/shrimp. At a dose of 50 mu g/shrimp, the recombinant protein provided protection as soon as 1 day after feeding (72.5% survival). Similar results were obtained with larger-sized shrimp. These results show that recombinant VP28 expressed in a Gram-positive bacterium is a potential oral vaccine against WSSV. (c) 2008 Elsevier Ltd. All rights reserved.
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