Article
Medicine, General & Internal
Blazej Chermula, Wieslawa Kranc, Piotr Celichowski, Boguslawa Stelmach, Hanna Piotrowska-Kempisty, Paul Mozdziak, Leszek Pawelczyk, Robert Zygmunt Spaczynski, Bartosz Kempisty
Summary: This study analyzes the epithelial origin of cumulus and granulosa cells in ovarian follicles, and identifies genes associated with epithelialization processes. The results suggest that these cells originate from the ovarian surface epithelium, and some gene expression profiles may have tumorigenic properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
G. D. Catandi, D. R. Bresnahan, S. O. Peters, K. J. Fresa, L. J. Maclellan, C. D. Broeckling, E. M. Carnevale
Summary: Many age-related changes in metabolite abundance suggest impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Xue Yang, Jun Ma, Luoyu Mo, Yan Xiong, Xianrong Xiong, Daoliang Lan, Wei Fu, Shi Yin
Summary: This study found that stanniocalcin-1 (STC1) is widely expressed in yak ovaries and plays a role in follicular development and hormone production. Inhibiting STC1 expression results in decreased proliferation and survival of yak granulosa cells and increased secretion of estrogen. Overexpression of STC1 partially restores granulosa cell proliferation and has opposite effects on progesterone and estrogen secretion. This study provides new insights into the role of STC1 in yak granulosa cells and the mechanism of yak reproduction.
Article
Obstetrics & Gynecology
Cem Demirel, Hale Goksever Celik, Firat Tulek, Kardelen Uzun, Ercan Bastu
Summary: This study aimed to assess whether dual trigger alters the ploidy chance of a blastocyst in PGT-A cycles, and found that dual triggering did not provide additional superiority on blastocyst euploidy rate compared to hCG triggering. Further studies in women with different infertility etiology are needed to evaluate the impact of triggering method on ploidy rates.
REPRODUCTIVE SCIENCES
(2022)
Article
Biology
Jakub Kulus, Magdalena Kulus, Wieslawa Kranc, Karol Jopek, Maciej Zdun, Malgorzata Jozkowiak, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Dorota Bukowska, Pawel Antosik, Paul Mozdziak, Bartosz Kempisty
Summary: The study focused on the gene expression profile of porcine granulosa cells during primary in vitro culture, particularly related to ECM formation and intercellular signaling. Most genes were upregulated, providing insight into molecular mechanisms within the ovary which can be used for assisted reproduction techniques and stemness potential of granulosa cells. The research may also serve as preliminary data for further studies on ovarian pathological processes using in vitro granulosa cell cultures.
Article
Biotechnology & Applied Microbiology
Ling Zhao, Yangyang Pan, Meng Wang, Junqian Wang, Yaying Wang, Xiaohong Han, Jinglei Wang, Tongxiang Zhang, Tian Zhao, Honghong He, Yan Cui, Sijiu Yu
Summary: A comprehensive whole-transcriptome analysis was conducted to understand the proliferation and differentiation of granulosa cells (GCs) and cumulus cells (CCs) in yaks. The study identified differentially expressed long non-coding RNA (lncRNA), microRNA (miRNA), and messenger RNA (mRNA) that play important roles in cell adhesion, cell differentiation, oocyte development, and other biological processes. These RNAs are involved in various signaling pathways and molecular interactions. The findings contribute to the understanding of oocyte development and provide a theoretical basis for future research.
Article
Obstetrics & Gynecology
A. Kedem, K. Ulanenko-Shenkar, Y. Yung, M. Youngster, S. Avraham, G. M. Yerushalmi, A. Hourvitz
Summary: This study confirms LUM as a newly discovered ovulatory gene that may play a crucial role in the preovulatory period leading up to ovulation, as well as in endometriosis infertility. A better understanding of LUM's role could potentially offer new treatment paradigms for certain types of female infertility.
REPRODUCTIVE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Bongkoch Turathum, Er-Meng Gao, Khwanthana Grataitong, Yu-Bing Liu, Ling Wang, Xue Dai, Ri-Cheng Chian
Summary: We evaluated the metabolic profiles of cumulus cells and mural granulosa cells derived from women with endometriosis, and found differences in sphingolipid metabolites between the two cell types. These differences may be involved in the regulation of oocyte quality.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Xianrong Xiong, Hongchen Ma, Xinyu Min, Feng Su, Yan Xiong, Jian Li
Summary: The study investigated the expression and function of lysine-specific demethylase 4B (KDM4B) in yak cumulus cells (CCs) and found that KDM4B regulates biological characteristics and physiological function of CCs mainly through changing the methylation patterns of H3K9 and related gene expression levels, affecting cell proliferation, apoptosis, hormone secretion, and embryo development.
Article
Health Care Sciences & Services
Ravindra Kolhe, Umar Ghilzai, Ashis K. Mondal, Chetan Pundkar, Pankaj Ahluwalia, Nikhil S. Sahajpal, Jie Chen, Carlos M. Isales, Mark Fulcher, Sadanand Fulzele
Summary: This study used Nanostring nCounter gene expression analysis to investigate the molecular signaling involved in trigger finger pathogenesis and identified dysregulated pathways, including upregulation of collagen genes and downregulation of MMP-3, as well as novel signaling pathways such as PI3K, MAPK, JAK-STAT, and Notch. The findings contribute to a better understanding of the pathogenesis of trigger finger.
Article
Cell Biology
Francesca P. Luongo, Filippo Dragoni, Adele Boccuto, Eugenio Paccagnini, Mariangela Gentile, Tamara Canosi, Giuseppe Morgante, Alice Luddi, Maurizio Zazzi, Ilaria Vicenti, Paola Piomboni
Summary: This study investigates the potential of SARS-CoV-2 to infect follicular ovarian cells and finds that these cells are susceptible to the virus. This suggests that COVID-19 infection may have a detrimental effect on female fertility.
Article
Obstetrics & Gynecology
Er-Meng Gao, Bongkoch Turathum, Ling Wang, Di Zhang, Yu-Bing Liu, Rong-Xin Tang, Ri-Cheng Chian
Summary: This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles. The metabolomics analysis revealed significantly different metabolites related to cholesterol transport, estradiol production, and antiapoptosis between CCs and MGCs. These differences may play a role in regulating oocyte maturation.
REPRODUCTIVE SCIENCES
(2022)
Article
Cell Biology
Hairui Fan, Zhanshi Ren, Chao Xu, Haifei Wang, Zhengchang Wu, Zia Ur Rehman, Shenglong Wu, Ming-an Sun, Wenbin Bao
Summary: Deoxynivalenol (DON) is a common environmental toxin secreted by fusarium fungi, which can induce cytotoxic effects and disrupt hormone secretion in ovarian granulosa cells (GCs). The effects may involve alterations in multiple signaling pathways and genes.
Article
Agriculture, Dairy & Animal Science
Marwa S. Faheem, Nasser Ghanem, Ahmed Gad, Radek Prochazka, Sherif M. Dessouki
Summary: The study revealed that buffalo granulosa cells exhibited different adaptive responses at the physiological and molecular levels under heat stress conditions, showing functional persistence and adaptability at 40.5 degrees C. This may provide insights into the biological mechanisms through which tropically adapted breeds react to heat stress.
Review
Cell Biology
Claudia Dompe, Magdalena Kulus, Katarzyna Stefanska, Wieslawa Kranc, Blazej Chermula, Rut Bryl, Wojciech Pienkowski, Mariusz J. Nawrocki, James N. Petitte, Boguslawa Stelmach, Paul Mozdziak, Michal Jeseta, Leszek Pawelczyk, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Robert Z. Spaczynski, Michal Nowicki, Bartosz Kempisty
Summary: The ovarian follicle is the basic functional unit of the ovary, consisting of theca cells and granulosa cells. Granulosa cells can be further divided into mural granulosa cells and cumulus cells, each playing different roles during folliculogenesis. Both types of cells exhibit stem-like properties and are influenced by various miRNAs, which may contribute to female fertility issues such as PCOS or POI. Further research on the cellular features and molecular pathways of granulosa and cumulus cells could lead to new strategies in enhancing assisted reproductive techniques.
Article
Obstetrics & Gynecology
Laura Emilie Vexo, Sacha Stormlund, Selma Kloeve Landersoe, Henrik Lovendahl Jorgensen, Peter Humaidan, Christina Bergh, Anne Lis Mikkelsen Englund, Anna Klajnbard, Jeanette Wulff Bogstad, Nina la Cour Freiesleben, Anne Zedeler, Lisbeth Praetorius, Anders Nyboe Andersen, Kristine Lossl, Anja Pinborg, Henriette Svarre Nielsen
Summary: Low-grade inflammation, detected by CRP, is associated with the outcome of IVF, including blastocyst quality and pregnancy outcome. Higher baseline CRP concentrations are negatively associated with live birth rate and are associated with pregnancy loss. However, there is no association between CRP concentrations and the number of good-quality blastocysts. More studies are needed to investigate the impact of low-grade inflammation.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Birgit Alsbjerg, Ulrik Schioler Kesmodel, Peter Humaidan
Summary: What is the optimal serum progesterone cut-off level in patients with endometriosis undergoing hormone replacement therapy frozen embryo transfer (HRT-FET) with intensive progesterone luteal phase support? The study revealed that serum progesterone levels above 118 nmol/l (37.1 ng/ml) resulted in significantly higher live birth rates compared to lower levels, indicating the existence of a threshold for optimal serum progesterone.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Jesus Catienas, Liv la Cour Poulsen, Linn Salto Mamsen, Claus Yding Andersen
Summary: In several mammalian species, oocytes from small antral follicles after in vitro maturation (IVM) are successfully used for procreation. Humans are the exception, mainly due to limited access to immature oocytes and unique regulation of oocyte maturation. However, recent findings suggest that human IVM can be developed, with factors such as oocyte diameter, follicle size, hormone levels, and cumulus-oocyte complex secretion influencing the maturation process. Furthermore, studies on signal transduction pathways and hormone levels during physiological conditions provide new insights for improving the current IVM platform. With continued research efforts, it is envisioned that a human IVM platform equally effective as in other mammalian species can be achieved. (Fertil Sterile 2023;119:550-9. (c) 2023 by American Society for Reproductive Medicine.)
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Esra Uyanik, Sezcan Mumusoglu, Mehtap Polat, Irem Yarali Ozbek, Sandro C. Esteves, Peter Humaidan, Hakan Yarali
Summary: A drop in serum P-4 level from oocyte pick-up (OPU) + 3 days to OPU + 5 days may result in a decrease in ongoing pregnancy rates. This decrease may be associated with lower initial pregnancy rates rather than increased overall pregnancy loss rates.
HUMAN REPRODUCTION
(2023)
Editorial Material
Obstetrics & Gynecology
Peter Humaidan, Juan Antonio Garcia Velasco, Mauro Cozzolino
FERTILITY AND STERILITY
(2023)
Review
Biochemistry & Molecular Biology
Monica Lispi, Peter Humaidan, George R. Bousfield, Thomas D'Hooghe, Alfredo Ulloa-Aguirre
Summary: Follicle-stimulating hormone (FSH), along with luteinizing hormone (LH) and human chorionic gonadotropin (hCG), is crucial for human reproduction. The discovery of FSH and other gonadotropins has significantly contributed to our knowledge of reproduction and the development of infertility treatments. However, the structural heterogeneity of FSH glycoforms influences the biological activity of human FSH products, and potency does not accurately predict the effects in terms of pharmacokinetics, pharmacodynamics, and clinical response in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Hakan Yarali, Sezcan Mumusoglu, Mehtap Polat, Murat Erden, Irem Yarali Ozbek, Sandro C. Esteves, Peter Humaidan
Summary: This study compared the effectiveness of subcutaneous progesterone and vaginal progesterone in frozen embryo transfer cycles. The results showed that subcutaneous progesterone was effective in maintaining an adequate serum progesterone level, while some patients who received vaginal progesterone required a rescue protocol to supplement progesterone levels. Both routes of administration had comparable ongoing pregnancy rates.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Malene Tanderup, Amrita Pande, Lone Schmidt, Birgitte B. Nielsen, Peter Humaidan, Charlotte Krolokke
Summary: This study examines the experiences of Danish permanently infertile couples when undergoing surrogacy abroad and the impact of the Ukraine war and COVID-19 pandemic. The findings reveal that most couples went to Ukraine due to the availability of enforceable contracts, professional advice, and the option to use the eggs of the intended mother. Current Danish legislation prevents the intended mother from obtaining legal motherhood, leading to feelings of inadequacy. The study highlights the importance of understanding cross-border experiences to inform legal policies and ensure reproductive opportunities for permanently infertile couples are not missed.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Obstetrics & Gynecology
J. M. Nielsen, P. Humaidan, M. B. Jensen, B. Alsbjerg
Summary: Bleeding before the eighth week of gestation is common in HRT-FET patients, but does not affect reproductive outcomes. Spontaneously conceived pregnancies have a 20% incidence of bleeding, most of which have normal outcomes.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
B. Alsbjerg, M. B. Jensen, B. B. Povlsen, H. O. Elbaek, R. J. Laursen, U. S. Kesmodel, P. Humaidan
Summary: Rectal administration of progesterone on the day of blastocyst transfer can effectively increase ongoing pregnancy rates in patients with low serum progesterone levels.
HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Mengxue Zheng, Jesus Cadenas, Susanne Elisabeth Pors, Tasnim Esa, Stine Gry Kristensen, Linn Salto Mamsen, Cristina Subiran Adrados, Claus Yding Andersen
Summary: This study aimed to optimize the culture conditions for murine preantral follicles to improve their growth and survival. The results showed that altering the alginate concentration, adding estradiol, and increasing follicle-stimulating hormone concentration can promote the growth and development of preantral follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
I. Feferkorn, S. Santos-Ribeiro, F. M. Ubaldi, J. G. Velasco, B. Ata, C. Blockeel, A. Conforti, S. C. Esteves, H. M. Fatemi, L. Gianaroli, M. Grynberg, P. Humaidan, G. T. Lainas, A. La Marca, C. LaTasha, R. Lathi, R. J. Norman, R. Orvieto, R. Paulson, A. Pellicer, N. P. Polyzos, M. Roque, S. K. Sunkara, S. L. Tan, B. Urman, C. Venetis, A. Weissman, H. Yarali, M. H. Dahan
Summary: Managing hyper-response in ovarian stimulation involves collecting a target number of 15-19 oocytes, avoiding GnRH agonists, starting with a dose of 150 IU/day in the first IVF cycle, and refraining from using ICoasting to decrease the risk of OHSS.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Obstetrics & Gynecology
Safak Olgan, Enver Kerem Dirican, Arif Can Ozsipahi, Mehmet Sakinci, Peter Humaidan
Summary: A model has been developed to predict low progesterone levels on the day of embryo transfer based on patient and cycle characteristics. Weight and estradiol levels on day 5 are independently associated with progesterone levels. The predictive performance is enhanced when these variables are considered sequentially or in combination.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2023)
Review
Endocrinology & Metabolism
Stefano Palomba, Flavia Costanzi, Scott M. Nelson, Donatella Caserta, Peter Humaidan
Summary: This study reviewed and analyzed different interventions for preventing and reducing OHSS in IVF patients. The most effective interventions were found to be GnRH-ant co-treatment and GnRH agonist triggering, with moderate evidence. Mild ovarian stimulation and dopaminergic agonists were also effective and safe interventions.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Medicine, General & Internal
Maria Buhl Borgstrom, Anne Ahrendt Bjerregaard, Sjurdur F. Olsen, Anette Gabrielsen, Peter Humaidan, Ulrik Schioler Kesmodel
Summary: This study aims to investigate the possible association between food intake and semen quality in men, as well as pregnancy and live birth rates in women undergoing intrauterine insemination or assisted reproductive technology treatment. Data will be collected through a web-based questionnaire and medical records, with a total of 4000 participants recruited. The results will be disseminated and published through various channels.
