期刊
FERTILITY AND STERILITY
卷 97, 期 5, 页码 1152-+出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2012.02.010
关键词
Stem cells; cryopreservation; vitrification; transplantation; male infertility; electron microscopy; spermatogenesis
资金
- Agency for Innovation by Science and Technology
- Flemish League Against Cancer-Public Utility Foundation
- Scientific Research Foundation Flanders, Methusalem
- Vrije Universiteit Brussel
Objective: To investigate whether solid-surface vitrification (SSV) is an effective cryopreservation strategy regarding the integrity and function of prepubertal mouse testicular tissue. Design: Prospective experimental study. Setting: Academic research unit. Animal(s): Mice. Intervention(s): Testicular tissue from 5- to 10-day-old GFP(+) mice was cryopreserved with the use of a conventional uncontrolled slow freezing (USF) technique and SSV before intratesticular grafting in busulfan-treated GFP(-) mice. Main Outcome Measure(s): Ultrastructural cryoinjury to spermatogonial stem cells (SSCs) and somatic cells was assessed by electron microscopy. Tubular structure was evaluated by histology, and graft survival and spermatogenic recovery by immunohistochemistry. Result(s): The tubular morphology and the proportion of ultrastructural cryodamage were similar between vitrified and slow-frozen testicular fragments. Allografting of tissue after both USF and SSV resulted in a recovery of spermatogenesis similar to fresh samples. Conclusion(s): SSV resulted in success rates similar to USF in maintaining testicular cell ultrastructure, tubular morphology, and tissue function. These data provide further evidence that vitrification, being an inexpensive and simple technique, can be considered as an alternative for cryopreservation of prepubertal testicular tissue. (Fertil Steril (R) 2012;97:1152-7. (C) 2012 by American Society for Reproductive Medicine.)
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