Article
Obstetrics & Gynecology
David J. McLernon, Edwin-Amalraj Raja, James P. Toner, Valerie L. Baker, Kevin J. Doody, David B. Seifer, Amy E. Sparks, Ethan Wantman, Paul C. Lin, Siladitya Bhattacharya, Bradley J. Van Voorhis
Summary: This study aims to develop in vitro fertilization (IVF) prediction models to estimate individualized chances of cumulative live birth, providing guidance for IVF treatment at different time points.
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
Adrija Kumar Datta, Stuart Campbell, Nirmal Felix, Jasdev Singh Harbhajan Singh, Geeta Nargund
Summary: For women with uncompromised ovarian reserve undergoing mild stimulation IVF, optimizing live birth rate (LBR) per cycle and cumulative LBR (CLBR) is achieved with 9 oocytes or 4 embryos for fresh transfers, and 12 oocytes or 9 embryos for predicted normal responders, while safeguarding against ovarian hyperstimulation syndrome (OHSS).
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Obstetrics & Gynecology
Anna-Karina Aaris Henningsen, Signe Opdahl, Ulla-Britt Wennerholm, Aila Tiitinen, Steen Rasmussen, Liv Bente Romundstad, Christina Bergh, Mika Gissler, Julie Lyng Forman, Anja Pinborg
Summary: This study investigated the risk of major congenital malformations in live-born singletons conceived with intracytoplasmic sperm injection (ICSI) compared to in vitro fertilization (IVF). The findings showed that the risk of major malformations was slightly higher in live-born singletons conceived with fresh ICSI compared to fresh IVF. Children conceived through ICSI also had a higher risk of respiratory and chromosomal malformations, but the number of cases was small in each group. The risk of hypospadias was higher when ICSI was performed solely due to male factor infertility. These findings should be considered when choosing assisted reproductive technology methods for couples without male factor infertility.
FERTILITY AND STERILITY
(2023)
Article
Endocrinology & Metabolism
Mingze Du, Junwei Zhang, Zhancai Wei, Li Li, Xinmi Liu, Manman Liu, Xingling Wang, Yichun Guan
Summary: A retrospective cohort study found no evidence that season and temperature on oocyte retrieval day affect the cumulative live birth rate and time to live birth.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Sladjana Malic Voncina, Amelie Stenqvist, Mona Bungum, Tommy Schyman, Aleksander Giwercman
Summary: It was found that couples with high sperm DNA fragmentation index (DFI) have a significantly lower cumulative live birth rate (CLBR) if in vitro fertilization (IVF) is applied in the first cycle of assisted reproduction, as opposed to intracytoplasmic sperm injection (ICSI).
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Wen Yao, Ying Chen, Haixia Yao, Qingyun Yao, Lan Wang, Meng Wang, Jing Yue
Summary: This retrospective cohort study investigated the relationship between uterine niche and reproductive outcomes of IVF/ICSI. The study found that women with uterine niche had lower live birth rate, positive HCG test rate, clinical pregnancy rate, and implantation rate during IVF/ICSI treatment. The study highlights the negative impact of uterine niche on fertility outcomes in women with cesarean section history.
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Irene Hervas, Alberto Pacheco, Rocio Rivera-Egea, Maria Gil Julia, Ana Navarro-Gomezlechon, Nicolas Garrido
Summary: The study of 1339 couples undergoing 2759 IVF/ICSI cycles showed that high SDF did not affect reproductive success of unselected couples using autologous oocytes in IVF/ICSI cycles.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Medicine, General & Internal
Rui Yang, Zi-Ru Niu, Li-Xue Chen, Ping Liu, Rong Li, Jie Qiao
Summary: This retrospective study analyzed 17,978 couples of first ovarian hyperstimulation IVF/ICSI cycles, revealing that prognosis factors affecting CLBR include female age, body mass index, duration of infertility years, infertility factors, controlled ovarian hyperstimulation protocol, the number of acquired oocytes, and number of transferrable embryos.
CHINESE MEDICAL JOURNAL
(2021)
Review
Obstetrics & Gynecology
Kenny A. Rodriguez-Wallberg, Hanna P. Nilsson, Tekla Lind
Summary: This study conducted a systematic review and meta-analysis to quantify the impact of unilateral oophorectomy (UO) on the rates of live birth and pregnancy in women undergoing IVF/ICSI. The results showed that women with previous UO had significantly lower rates of live birth and pregnancy, as well as higher doses of administered gonadotropins and a lower number of retrieved oocytes.
FERTILITY AND STERILITY
(2022)
Article
Endocrinology & Metabolism
Meng Rao, Li Tang, Longda Wang, Mengxiang Chen, Gaofeng Yan, Shuhua Zhao
Summary: This retrospective study compared the effects of density gradient centrifugation (DGC) and swim-up (SU) on the outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). After matching and adjustment, no significant differences were found between the DGC and SU groups in terms of sperm recovery rate, progressive motility rate, fertilization rate, embryo quality, and blastocyst formation rate. The cumulative live birth rate and live birth rate per transfer were also not significantly different between the groups. However, the DGC group had a significantly higher fertilization rate when using poor-quality sperm compared to the SU group.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Review
Obstetrics & Gynecology
Yin Jun Law, Ning Zhang, Efstratios M. Kolibianakis, Michael F. Costello, Elena Keller, Georgina M. Chambers, Christos A. Venetis
Summary: This systematic review suggests that the optimal number of oocytes for maximizing fresh live birth rate is between 12 and 18 oocytes. However, cumulative live birth rate continues to increase with the number of oocytes retrieved.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Endocrinology & Metabolism
Enqi Yan, Wenxuan Li, Huizi Jin, Mengya Zhao, Dan Chen, Xinyao Hu, Yifan Chu, Yaxin Guo, Lei Jin
Summary: The aim of this study was to describe the cumulative live birth rates (CLBRs) of young women with or without low prognosis according to the POSEIDON criteria after IVF/ICSI cycles and to investigate whether the diagnosis of low prognosis increases the risk of abnormal birth outcomes. The study found that the CLBRs in the POSEIDON group were lower than the non-POSEIDON group, but the risk of abnormal birth outcomes did not increase in the POSEIDON group.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Physiology
Zhiyan Chen, Duoduo Zhang, Zhengyi Sun, Qi Yu
Summary: This study investigated the relationship between testosterone levels and pregnancy outcomes in patients with tubal or male infertility undergoing IVF/ICSI cycles. Results showed that an increase in testosterone levels was associated with higher numbers of retrieved oocytes and embryos, as well as better pregnancy outcomes. Additionally, faster increase in testosterone levels might lead to improved chances of live birth.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Xiaokun Hu, Yuliang Liu, Xiubing Zhang, Pingyin Lee, Yangxing Wen, Chenhui Ding, Canquan Zhou, Yanwen Xu
Summary: This study aimed to investigate the impact of oocyte degeneration after ICSI on cumulative live birth rate in young women; The results showed that oocyte degeneration is not an indicator for predicting the cumulative live birth rate.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Bruno Toson, Carlos Simon, Inmaculada Moreno
Summary: Changes in the female genital tract microbiome are related to gynecological and obstetrical pathologies, but the influence of endometrial bacterial communities on human reproduction is still debated. Understanding the impact of microbiome on fertility allows personalized treatment and improves clinical outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Tiffany Stankewicz, Maria Ruiz-Alonso, Marc Soler-Ibanez, Carlos Simon, Diana Valbuena
Summary: The study found that there is no difference in clinical outcomes between day-5 and day-6 blastocysts when transferred during the optimal endometrial receptivity as determined by ERA. This suggests that both day-5 and day-6 blastocysts have similar clinical potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Obstetrics & Gynecology
Carlos Simon
Summary: The application of transcriptomics and 16S rRNA gene sequencing technologies provide important foundations for personalized reproductive care. These technologies accurately identify the receptive period of the maternal endometrium for embryo implantation, as well as the association between microbial colonization in the endometrium and reproductive outcomes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Microbiology
Inmaculada Moreno, Iolanda Garcia-Grau, David Perez-Villaroya, Marta Gonzalez-Monfort, Mustafa Bahceci, Marcelo J. Barrionuevo, Sagiri Taguchi, Elena Puente, Michael Dimattina, Mei Wei Lim, Georgina Meneghini, Mira Aubuchon, Mark Leondires, Alexandra Izquierdo, Martina Perez-Olgiati, Alejandro Chavez, Ken Seethram, Davide Bau, Carlos Gomez, Diana Valbuena, Felipe Vilella, Carlos Simon
Summary: The composition of the endometrial microbiota is associated with reproductive outcomes, and can serve as a useful biomarker for predicting reproductive outcome.
Article
Obstetrics & Gynecology
Cesar Diaz-Garcia, Sonia Herraiz, Loida Pamplona, Jessica Subira, Maria Jose Soriano, Carlos Simon, Emre Seli, Antonio Pellicer
Summary: Ovarian fragmentation for follicular activation increases antral follicle counts in women with poor ovarian response, but does not significantly modify in vitro fertilization outcomes.
FERTILITY AND STERILITY
(2022)
Letter
Obstetrics & Gynecology
Carlos Simon, Maria Ruiz-Alonso, Carlos Gomez, Diana Valbuena
FERTILITY AND STERILITY
(2023)
Review
Medicine, Research & Experimental
Javier Gonzalez Fernandez, Javier Moncayo Arlandi, Ana Ochando, Carlos Simon, Felipe Vilella
Summary: Embryo-maternal cross-talk is crucial for embryo development and implantation, involving secreted factors and extracellular vesicles (EVs). EV cargo influences target cells and prepares them for reproductive stages like embryo development and implantation. Recent research suggests that EVs and their cargo can be used as a powerful, non-invasive tool to assess the quality of embryos and maternal tissues in assisted reproduction treatments. This review focuses on intercellular cross-talk between embryos and maternal tissues, examining human biology and signaling mechanisms identified in animal models.
Article
Obstetrics & Gynecology
Stephanie Bissiere, Blake Hernandez, Carmen Rubio, Carlos Simon, Nicolas Plachta
Summary: Preimplantation development is the stage of human development that can be observed and studied in real time outside the body. This is possible because human embryos can be created using in vitro fertilization and cultured until the blastocyst stage. This article focuses on the cellular and morphogenetic processes that transform a 1-cell embryo into a blastocyst ready for implantation. While most of our understanding of preimplantation human development comes from studying mouse embryos, this article discusses the potential differences in humans and highlights new approaches for studying this process using noninvasive imaging and genetic technologies.
FERTILITY AND STERILITY
(2023)
Article
Obstetrics & Gynecology
Laura Girardi, Matteo Figliuzzi, Maurizio Poli, Munevver Serdarogullari, Cristina Patassini, Silvia Caroselli, Ilaria Pergher, Francesco Cogo, Onder Coban, Fazilet Kubra Boynukalin, Mustafa Bahceci, Roser Navarro, Carmen Rubio, Necati Findikli, Carlos Simon, Antonio Capalbo
Summary: How well can whole chromosome copy number analysis from a single trophectoderm biopsy predict true mosaicism configurations in human blastocysts? When a single trophectoderm biopsy is tested, wide mosaicism thresholds increase false positive calls compared to more stringent ones without improving true detection rate, while binary classification provides the highest diagnostic accuracy.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
S. Caroselli, M. Figliuzzi, L. Picchetta, F. Cogo, P. Zambon, I Pergher, L. Girardi, C. Patassini, M. Poli, D. Bakalova, D. Cimadomo, N. Findikli, O. Coban, M. Serdarogullari, F. Favero, S. Bortolato, A. Anastasi, F. Capodanno, A. Gallinelli, F. Brancati, L. Rienzi, F. M. Ubaldi, J. Jimenez-Almazan, D. Blesa-Jarque, J. Miravet-Valenciano, C. Rubio, C. Simon, A. Capalbo
Summary: This study developed an integrated genetic testing platform that accurately detects chromosomal abnormalities, including ploidy level and microdeletions, expanding the clinical utility of preimplantation genetic testing.
HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Jose Ramon Hernandez Mora, Claudia Buhigas, Stephen Clark, Raquel Del Gallego Bonilla, Dagne Daskeviciute, Ana Monteagudo-Sanchez, Maria Eugenia Poo-Llanillo, Jose Vicente Medrano, Carlos Simon, Marcos Meseguer, Gavin Kelsey, David Monk
Summary: During pre-implantation stages, stored material in mammalian development promotes epigenetic profile erasure and genome activation. Through single-cell methylome and transcriptome sequencing, we analyzed the expression and methylation in oocytes and human embryos, identifying key events in epigenetic reprogramming and genome activation. Failure to achieve these milestones negatively impacts the developmental potential of embryos and may affect the success of assisted reproductive cycles.
Article
Biochemistry & Molecular Biology
Ana Domingo-Muelas, Robin M. Skory, Adam A. Moverley, Goli Ardestani, Oz Pomp, Carmen Rubio, Piotr Tetlak, Blake Hernandez, Eric A. Rhon-Calderon, Luis Navarro-Sanchez, Carmen M. Garcia-Pascual, Stephanie Bissiere, Marisa S. Bartolomei, Denny Sakkas, Carlos Simon, Nicolas Plachta
Summary: Combining fluorescent dyes with live imaging, this study reveals the dynamics of early development in human embryos, including chromosome segregation, compaction, polarization, blastocyst formation, and hatching. The study also shows that blastocyst expansion mechanically affects trophectoderm cells, causing nuclear budding and DNA shedding into the cytoplasm. Moreover, the study suggests that aneuploidies in human embryos may not only result from chromosome segregation errors during mitosis, but also from nuclear DNA shedding.
Article
Urology & Nephrology
Sarah Kimmins, Richard A. Anderson, Christopher L. R. Barratt, Hermann M. Behre, Sarah R. Catford, Christopher J. De Jonge, Geraldine Delbes, Michael L. Eisenberg, Nicolas Garrido, Brendan J. Houston, Niels Jorgensen, Csilla Krausz, Ariane Lismer, Robert I. Mclachlan, Suks Minhas, Tim Moss, Allan Pacey, Laerke Priskorn, Stefan Schlatt, Jacquetta Trasler, Leonardo Trasande, Frank Tuettelmann, Monica Hebe Vazquez-Levin, Joris A. Veltman, Feng Zhang, Moira K. O'Bryan
Summary: Currently, there is a lack of understanding and awareness about the causes of male infertility and the importance of male reproductive health. This leads to standardized treatment strategies using medically assisted reproduction techniques, which overlook the potential co-morbidities and shift the burden to women. To improve male reproductive health, it is essential to establish centers for data and sample collection and study the effects of genetics, epigenetics, and environment on fertility and offspring health. Personalized treatment protocols should be developed and education about infertility should be increased.
NATURE REVIEWS UROLOGY
(2023)
Review
Physiology
Inmaculada Moreno, Antonio Capalbo, Aymara Mas, Tamara Garrido-Gomez, Beatriz Roson, Maurizio Poli, Eva Dimitriadis, Xavier Santamaria, Felipe Vilella, Carlos Simon
Summary: Pregnancy is established during the periconceptional period from blastocyst attachment to placenta formation, significantly impacting the health of both the child and the mother. Advances in understanding the periconceptional space, including the preimplantation human embryo and maternal endometrium, offer opportunities for preventative interventions. This review discusses the role of maternal decidua, the maternal-embryonic interface, the endometrial microbiome, and the myometrium in pregnancy health during the periconceptional period.
PHYSIOLOGICAL REVIEWS
(2023)
Review
Obstetrics & Gynecology
Luis Navarro-Sanchez, Carmen Garcia-Pascual, Carmen Rubio, Carlos Simon
Summary: Aneuploidy is common among preimplantation human embryos used in assisted reproductive technology, and current testing methods involve invasive biopsy. However, emerging evidence suggests that embryos secrete cell-free DNA into their culture medium, indicating the potential for a non-invasive assay for aneuploidy. Embryonic cell-free DNA analysis shows high concordance with current methods and can help prioritize blastocysts for transfer, increasing implantation rates and reducing miscarriage rates and time to achieve pregnancy.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
