期刊
FERTILITY AND STERILITY
卷 95, 期 1, 页码 129-134出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2010.06.076
关键词
Bisulfite PCR-Luminex methylation analysis; BPL; DNA methylation; genomic imprinting; oligozoospermia; sperm
资金
- Ministry of Health and Welfare of Japan [19390423, 20017003, 21028003, H21-136]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [23013003, 23390385, 23659768, 23791799] Funding Source: KAKEN
Objective: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. Design: Experimental prospective study. Setting: University research laboratory and private in vitro fertilization (IVF) clinic. Patient(s): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. Intervention(s): The ejaculated sperm samples after the routine semen analysis with patients' consent. Main Outcome Measure(s): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). Result(s): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). Conclusion(s): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments. (Fertil Steril(R) 2011;95:129-34. (C) 2011 by American Society for Reproductive Medicine.)
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