Article
Andrology
Ana Timermans, Rosana Vazquez, Fatima Otero, Jaime Gosalvez, Stephen Johnston, Jose Luis Fernandez
Summary: The experiments found that ciprofloxacin and doxycycline significantly increased sperm DNA fragmentation rate, while ampicillin had no effect. The dynamic SDF assay is a rapid and sensitive tool to demonstrate sperm toxicity.
Article
Andrology
Ana Timermans, Fatima Otero, Manuel Garrido, Jaime Gosalvez, Stephen Johnston, Jose Luis Fernandez
Summary: The study found that there is no correlation between sperm mitochondrial DNA fragmentation and copy number with nuclear DNA fragmentation levels, while non-normozoospermic samples showed higher levels of nuclear DNA fragmentation and more mtDNA copy number compared to normozoospermic samples.
Article
Biochemistry & Molecular Biology
Stanislav Tjagur, Reet Maendar, Olev Poolamets, Kristjan Pomm, Margus Punab
Summary: The study aimed to investigate the prevalence of STIs among infertile males and their impact on semen parameters and blood PSA. The results showed that Mycoplasma genitalium is associated with seminal inflammation, and further research is needed to explore the impact of STIs on semen parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Andrology
Jaime Gosalvez, Carmen Lopez-Fernandez, Jose Luis Fernandez, Stephen Johnston
Summary: Microencapsulation using alginate has shown significant improvement in sperm membrane integrity and DNA fragmentation in human sperm after 24-hour storage, with potential applications in reproductive medicine. The technique has the potential to enhance sperm survival rates and is a promising approach for human fertility treatment.
Article
Environmental Sciences
Jonathan Kiwitt-Cardenas, Evdochia Adoamnei, Julian J. Arense-Gonzalo, Laura Sarabia-Cos, Fernando Vela-Soria, Mariana F. Fernandez, Jaime Gosalvez, Jaime Mendiola, Alberto M. Torres-Cantero
Summary: A study conducted with university students in the region of Murcia, Spain, found a significant positive association between urinary BPA concentrations and sperm DNA fragmentation in men with a high SDF index. However, further research is needed to confirm these results and draw conclusions in other male populations.
ENVIRONMENTAL RESEARCH
(2021)
Review
Andrology
Kareim Khalafalla, Ahmad Majzoub, Haitham Elbardisi, Ashni Bhathella, Arth Chaudhari, Ashok Agarwal, Ralf Henkel, Thoraya AlMarzooki, Hasan Burjaq, Mohamed Arafa
Summary: This study aimed to assess the impact of different levels of sperm DNA fragmentation on the outcome of clinical intracytoplasmic sperm injection. Results showed that in cases where sperm DNA fragmentation exceeded 30%, favorable females with older age or lower anti-Mullerian hormone levels had significantly higher clinical pregnancy and live birth rates compared to unfavorable females.
Article
Obstetrics & Gynecology
Jiangman Gao, Renpei Yuan, Siwei Yang, Yuanyuan Wang, Ying Huang, Liying Yan, Hui Jiang, Jie Qiao
Summary: The study analyzed the correlations between male age, traditional semen parameters, sperm DNA fragmentation index (DFI), and high DNA stainability (HDS) in a large sample size. The results showed that age negatively impacted semen volume, total sperm count, motility, and HDS, while positively affecting sperm concentration and DFI. The study concluded that aging affects sperm quantity and quality, and there are significant correlations between routine sperm parameters and sperm DNA/chromatin integrity.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Reproductive Biology
Jordi Ribas-Maynou, Estela Garcia-Bonavila, Sergi Bonet, Jaime Catalan, Albert Salas-Huetos, Marc Yeste
Summary: The relationship between sperm DNA fragmentation and reproductive outcomes is inconsistent, and concerns have been raised about its value as a sperm quality parameter. This study found that increasing chromatin decondensation makes the TUNEL assay more sensitive to detect DNA damage in pig sperm.
Article
Reproductive Biology
Jaime Gosalvez, Javier Bartolome-Nebreda, Eva Tvrda, Carmen Lopez-Fernandez, Jose Luis Fernandez, Stephen Johnston
Summary: The study aimed to investigate the relationship between DNase activity associated with bacterial contamination of incubated bovine frozen-thawed spermatozoa and elevated sperm DNA fragmentation. The experiments showed that incubation of plasmid with the supernatant of infected bovine semen straws resulted in clear evidence of DNase activity compared to non-infected supernatant. The presence of bacteria in semen straws led to an exponential increase in bacterial growth and sperm DNA fragmentation, highlighting the importance of hygiene practices during semen processing.
Article
Veterinary Sciences
Jordi Ribas-Maynou, Marc Llavanera, Yentel Mateo-Otero, Estela Garcia-Bonavila, Ariadna Delgado-Bermudez, Marc Yeste
Summary: This study analyzed eight techniques assessing chromatin damage in pig sperm and found that the extent of chromatin damage is significantly correlated with the percentage of sperm with fragmented DNA in direct methods, but not in indirect ones. The results suggest that there are differences in interpreting sperm chromatin damage between indirect and direct techniques.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
Hieu Nguyen, Jordi Ribas-Maynou, Hongwen Wu, Beverly Quon, Tracy Inouye, Brienne Walker, Caitlin Langaman, Thomas T. F. Huang Jr, W. Steven Ward
Summary: We previously demonstrated that MnCl2 induces double-stranded DNA breaks in sperm, which we term as sperm chromatin fragmentation. Here, we compared the levels of double-stranded DNA breaks induced by MnCl2 to those induced by H2O2, a agent that causes single-stranded DNA breaks. Both methods showed concentration-dependent induction of DNA breaks.
BIOLOGY OF REPRODUCTION
(2023)
Article
Andrology
Mojdeh Sabour, Azam Agha-Rahimi, Mahmood Dehghani Ashkezari, Seyed Morteza Seifati, Fatemeh Anbari, Ali Nabi
Summary: Polyvinylpyrrolidone (PVP) is commonly used in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. However, this study found that prolonged exposure to PVP significantly affects sperm viability and morphology, leading to increased DNA fragmentation and abnormal chromatin structure.
Article
Agriculture, Dairy & Animal Science
Jordi Ribas-Maynou, Ariadna Delgado-Bermudez, Yentel Mateo-Otero, Estel Vinolas, Carlos O. Hidalgo, W. Steven Ward, Marc Yeste
Summary: This study analyzed cryopreserved bovine sperm after thawing and found that DNA damage and sperm degradation are influenced by oxidative stress. Evaluating chromatin integrity can help predict bull fertility.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Medicine, General & Internal
Christophe Depuydt, Eugene Bosmans, Jef Jonckheere, Francesca Donders, Willem Ombelet, Astrid Coppens, Gilbert Donders
Summary: This longitudinal observational cohort study found that SARS-CoV-2 infection has differential effects on sperm quality and fertility, including parameters such as motility, morphology, DNA damage, and anti-sperm antibodies. These effects may vary among individuals. However, most sperm parameters return to normal after infection.
Article
Developmental Biology
Jordi Ribas-Maynou, Hieu Nguyen, Raquel Valle, Hongwen Wu, Marc Yeste, W. Steven Ward
Summary: Vasectomy can activate sperm chromatin fragmentation (SCF) as a mechanism to degrade sperm DNA that cannot be ejaculated, preventing their function. The study found that double-strand breaks were increased in vas deferens sperm after vasectomy, mainly occurring in the toroid linker regions (TLRs).
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Andrology
E. Tvrda, C. Lopez-Fernandez, P. Sanchez-Martin, J. Gosalvez
Article
Genetics & Heredity
Anais Garcia Rodriguez, Moises de la Casa, Stephen Johnston, Jaime Gosalvez, Rosa Roy
ANNALS OF HUMAN GENETICS
(2019)
Review
Biochemistry & Molecular Biology
Anais Garcia-Rodriguez, Jaime Gosalvez, Ashok Agarwal, Rosa Roy, Stephen Johnston
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Veterinary Sciences
Blasa C. Pereira, Isabel Ortiz, Jesus Dorado, Cesar Consuegra, Maria Diaz-Jimenez, Sebastian Demyda-Peyras, Jaime Gosalvez, Manuel Hidalgo
JOURNAL OF EQUINE VETERINARY SCIENCE
(2019)
Article
Genetics & Heredity
Eva Tvrda, Francisca Arroyo, Michal Duracka, Carmen Lopez-Fernandez, Jaime Gosalvez
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2019)
Article
Agriculture, Dairy & Animal Science
J. de la Torre, F. Crespo, F. Arroyo, M. Zabal-Aguirre, A. S. Abdoon, J. Gosalvez
ANIMAL REPRODUCTION SCIENCE
(2019)
Article
Biotechnology & Applied Microbiology
E. Cortes-Gutierrez, C. Garcia-Vielma, M. Davila-Rodriguez, H. Sanchez-Davila, J. L. Fernandez, J. Gosalvez
BIOTECHNIC & HISTOCHEMISTRY
(2020)
Article
Agriculture, Dairy & Animal Science
Blasa Carmen Pereira, Isabel Ortiz, Jesus Manuel Dorado, Maria Angeles Diaz-Jimenez, Cesar Consuegra, Jaime Gosalvez, Manuel Hidalgo
REPRODUCTION IN DOMESTIC ANIMALS
(2019)
Article
Andrology
Elva Cortes-Gutierrez, Carlos Garcia De La Vega, Javier Bartolome-Nebreda, Jaime Gosalvez
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2019)
Article
Andrology
Jaime Gosalvez, Carmen Lopez-Fernandez, Jose Luis Fernandez, Stephen Johnston
Summary: Microencapsulation using alginate has shown significant improvement in sperm membrane integrity and DNA fragmentation in human sperm after 24-hour storage, with potential applications in reproductive medicine. The technique has the potential to enhance sperm survival rates and is a promising approach for human fertility treatment.
Review
Andrology
Sandro C. Esteves, Armand Zini, Robert Matthew Coward, Donald P. Evenson, Jaime Gosalvez, Sheena E. M. Lewis, Rakesh Sharma, Peter Humaidan
Summary: This article summarizes the impact of sperm DNA fragmentation in various clinical infertility scenarios and advances in sperm DNA fragmentation tests. Based on collected evidence, 41 recommendations were formulated, covering technical aspects, clinical thresholds, interpretation of results, indications for testing, and clinical management. The bulk of evidence supporting these recommendations has increased in recent years, although it is still of moderate to low quality.
Article
Developmental Biology
S. D. Johnston, C. Lopez-Fernandez, F. Arroyo, R. Roy, W. V. Holt, J. Gosalvez
REPRODUCTION FERTILITY AND DEVELOPMENT
(2019)
Review
Andrology
Ashok Agarwal, Neel Parekh, Manesh Kumar Panner Selvam, Ralf Henkel, Rupin Shah, Sheryl T. Homa, Ranjith Ramasamy, Edmund Ko, Kelton Tremellen, Sandro Esteves, Ahmad Majzoub, Juan G. Alvarez, David K. Gardner, Channa N. Jayasena, Jonathan W. Ramsay, Chak-Lam Cho, Ramadan Saleh, Denny Sakkas, James M. Hotaling, Scott D. Lundy, Sarah Vij, Joel Marmar, Jaime Gosalvez, Edmund Sabanegh, Hyun Jun Park, Armand Zini, Parviz Kavoussi, Sava Micic, Ryan Smith, Gian Maria Busetto, Mustafa Emre Bakircioglu, Gerhard Haidl, Giancarlo Balercia, Nicols Garrido Puchalt, Moncef Ben-Khalifa, Nicholas Tadros, Jackson Kirkman-Browne, Sergey Moskovtsev, Xuefeng Huang, Edson Borges, Daniel Franken, Natan Bar-Chama, Yoshiharu Morimoto, Kazuhisa Tomita, Vasan Satya Srini, Willem Ombelet, Elisabetta Baldi, Monica Muratori, Yasushi Yumura, Sandro La Vignera, Raghavender Kosgi, Marlon P. Martinez, Donald P. Evenson, Daniel Suslik Zylbersztejn, Matheus Roque, Marcello Cocuzza, Marcelo Vieira, Assaf Ben-Meir, Raoul Orvieto, Eliahu Levitas, Amir Wiser, Mohamed Arafa, Vineet Malhotra, Sijo Joseph Parekattil, Haitham Elbardisi, Luiz Carvalho, Rima Dada, Christophe Sifer, Pankaj Talwar, Ahmet Gudeloglu, Ahmed M. A. Mahmoud, Khaled Terras, Chadi Yazbeck, Bojanic Nebojsa, Damayanthi Durairajanayagam, Ajina Mounir, Linda G. Kahn, Saradha Baskaran, Rishma Dhillon Pai, Donatella Paoli, Kristian Leisegang, Mohamed-Reza Moein, Sonia Malik, Onder Yaman, Luna Samanta, Fouad Bayane, Sunil K. Jindal, Muammer Kendirci, Baris Altay, Dragoljub Perovic, Avi Harlev
WORLD JOURNAL OF MENS HEALTH
(2019)
Article
Genetics & Heredity
A. Garcia-Rodriguez, M. de la Casa, J. Gosalvez, R. Roy
Meeting Abstract
Agriculture, Dairy & Animal Science
L. Gutierrez-Cepeda, C. Serres, A. Fernandez, J. Gosalvez, J. C. Blazquez, F. Crespo
REPRODUCTION IN DOMESTIC ANIMALS
(2018)