Article
Genetics & Heredity
Adrien Bloch, Eli J. Rogers, Cynthia Nicolas, Tanguy Martin-Denavit, Miguel Monteiro, Daniel Thomas, Helene Morel, Rachel Levy, Jean-Pierre Siffroi, Charlotte Dupont, Alexandre Rouen
Summary: The study compared the quality differences between different HOST classes, identifying HOST classes B and B+ as having the highest quality based on various metrics. This opens up possibilities for sperm selection before ICSI, potentially optimizing reproductive outcomes in unstudied patient populations.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Reproductive Biology
Jordi Ribas-Maynou, Estela Garcia-Bonavila, Sergi Bonet, Jaime Catalan, Albert Salas-Huetos, Marc Yeste
Summary: The relationship between sperm DNA fragmentation and reproductive outcomes is inconsistent, and concerns have been raised about its value as a sperm quality parameter. This study found that increasing chromatin decondensation makes the TUNEL assay more sensitive to detect DNA damage in pig sperm.
Article
Andrology
Prabir Mondal, Ratnabali Maity, Chhanda Mallick
Summary: The study found that the aqua-methanolic extract of Thevetia peruviana leaves exhibited a significant spermicidal effect in an in vitro model. Sperm motility was immediately affected at a dose of 20 mg/ml, gradually reduced at doses of 40 and 80 mg/ml, and completely immobilized at a dose of 160 mg/ml within 5 minutes of exposure.
Article
Agriculture, Dairy & Animal Science
Sylwia Prochowska, Wojciech Nizanski, Alain Fontbonne
Summary: The hypo-osmotic swelling test (HOST) is a method used to assess semen quality. This study aimed to evaluate a simplified HOST procedure for feline semen and determine if sperm abnormalities could affect the test results. The results showed that the simplified HOST gave the same results as the standard one and that abnormal spermatozoa did not interfere with the test results.
Article
Developmental Biology
B. Bernal, A. Behnamifar, C. Alvarez-Rodriguez, A. Toledano-Diaz, C. Castano, R. Velazquez, M. G. Gil, A. Gutierrez-Adan, Henri Woelders, E. Blesbois, J. Santiago-Moreno
Summary: This study aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract and to study the vas deferens lining cells potentially involved in sperm maturation. The results showed that the transit though the vas deferens resulted in a higher percentage of compacted chromatin spermatozoa in the ejaculate.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Oncology
Daniel Moreno-Andres, Kristin Holl, Wolfram Antonin
Summary: The nucleus undergoes significant changes during cell division, such as the breakdown of the nuclear envelope and rearrangement of chromosomes. While the first half of mitosis has been extensively studied, less is known about the reformation of a functional nucleus in each emerging cell. This review focuses on mitotic exit and nuclear reformation, with emphasis on their relevance to cancer biology.
SEMINARS IN CANCER BIOLOGY
(2023)
Article
Andrology
Mingzhe Song, Wensi Huang, Lijun Ye, Zhiqiang Liu, Weiqiang Xiao, Feng Xiong, Yueqin Peng, Jing Cai, Yong Zeng
Summary: The study aimed to investigate the association between high-DNA stainability (HDS) and embryonic development and clinical outcomes after IVF treatment. The results showed a negative correlation between HDS and fertilization rate, but no correlation was observed after adjusting confounding factors. Furthermore, there were no significant differences in clinical pregnancy rate, ongoing pregnancy rate, miscarriage rates, and live birth rate between the HDS groups. Therefore, HDS evaluation may have limited potential in predicting embryo development and clinical outcomes.
Article
Biochemistry & Molecular Biology
Gennaro Lettieri, Rosaria Notariale, Alessia Ambrosino, Alfredo Di Bonito, Antonella Giarra, Marco Trifuoggi, Caterina Manna, Marina Piscopo
Summary: This study investigates the effects of exposure to different concentrations of mercury ions on sperm and PL proteins of Mytilus galloprovincialis, revealing potential DNA damage and adverse effects on the reproductive system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ming-Lin Liu, Xing Lyu, Victoria P. Werth
Summary: The article discusses the formation of neutrophil extracellular traps (NETs) and their relationship with immune, autoimmune responses, and the development of various human diseases. It emphasizes the anti-infective role of externalized chromatin in animals ranging from lower invertebrates to higher vertebrates, while also pointing out potential issues in higher vertebrate animals.
Article
Cell Biology
Yuki Hara
Summary: The eukaryotic nucleus can change its size dynamically in response to cellular conditions. Supply of structural constituents and physical forces such as osmotic pressure and chromatin repulsion play crucial roles in regulating nuclear size. Multiple nuclear macromolecules beyond the structural constituents of the nuclear envelope are involved in controlling nuclear size and related functions.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Medicine, General & Internal
Li Liu, Kechong Zhou, Yuxuan Song, Xiaoqiang Liu
Summary: In this study, novel mutations in the CCDC40 gene were identified in a Chinese family with PCD, leading to immobility of sperm and infertility. The safety and effectiveness of ICSI for infertility caused by PCD were confirmed. The combination of targeted next generation sequencing and Sanger sequencing proved to be useful for diagnosing PCD, indicating potential for genetic diagnosis in PCD patients.
Review
Biochemistry & Molecular Biology
Rhianna Davies, Channa N. N. Jayasena, Raj Rai, Suks Minhas
Summary: Recurrent pregnancy loss is a distressing condition affecting 1-2% of couples. Studies suggest that elevated seminal reactive oxygen species may contribute to recurrent pregnancy loss by causing damage to sperm. However, there is conflicting data and limitations in study methodologies. The effects of oxidative stress on the epigenome and the value of antioxidant therapy in managing recurrent pregnancy loss remain unclear.
Review
Cell Biology
Fernanda Fuentes, Erwin Munoz, Maria Jose Contreras, Maria Elena Arias, Ricardo Felmer
Summary: Intracytoplasmic sperm injection (ICSI) is not yet optimal in cattle, but recent advancements in artificial activation treatments and sperm capacitation have shown promise. This review explores various strategies to improve the efficiency of bovine ICSI and proposes alternative approaches.
Article
Andrology
Andrea Busnelli, Andrea Garolla, Elena Di Credico, Silvia D'Ippolito, Anna Maria Merola, Domenico Milardi, Alfredo Pontecorvi, Giovanni Scambia, Nicoletta Di Simone
Summary: This study found that sperm DNA fragmentation is significantly higher in men associated with idiopathic recurrent pregnancy loss, but not with infertility. These findings provide important evidence for the role of sperm DNA fragmentation in idiopathic recurrent pregnancy loss.
Review
Andrology
Annalisa Inversetti, Arianna Bossi, Martina Cristodoro, Alessandro Larcher, Andrea Busnelli, Giuseppe Grande, Andrea Salonia, Nicoletta Di Simone
Summary: The study found a correlation between sperm DNA fragmentation, semen parameters, and recurrent pregnancy loss in multiple ethnic groups.
Article
Biochemistry & Molecular Biology
Hye Shin Lee, Mujeeburahiman Cheerathodi, Sankar P. Chaki, Steve B. Reyes, Yanhua Zheng, Zhimin Lu, Helena Paidassi, Celine DerMardirossian, Adam Lacy-Hulbert, Gonzalo M. Rivera, Joseph H. McCarty
MOLECULAR AND CELLULAR BIOLOGY
(2015)
Article
Cell Biology
David C. Morris, Julia L. Popp, Leung K. Tang, Holly C. Gibbs, Emily Schmitt, Sankar P. Chaki, Briana C. Bywaters, Alvin T. Yeh, Weston W. Porter, Robert C. Burghardt, Rola Barhoumi, Gonzalo M. Rivera
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Multidisciplinary Sciences
Martha E. Hensel, Daniel G. Garcia-Gonzalez, Sankar P. Chaki, James Samuel, Angela M. Arenas-Gamboa
Article
Immunology
Omar H. Khalaf, Sankar P. Chaki, Daniel G. Garcia-Gonzalez, Thomas A. Ficht, Angela M. Arenas-Gamboa
INFECTION AND IMMUNITY
(2019)
Correction
Multidisciplinary Sciences
Martha E. Hensel, Daniel G. Garcia-Gonzalez, Sankar P. Chaki, James Samuel, Angela M. Arenas-Gamboa
Article
Oncology
Sankar P. Chaki, Rola Barhoumi, Gonzalo M. Rivera
Article
Immunology
Omar H. Khalaf, Sankar P. Chaki, Daniel G. Garcia-Gonzalez, Larry J. Suva, Dana Gaddy, Angela M. Arenas-Gamboa
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Lauren W. Stranahan, Sankar P. Chaki, Daniel G. Garcia-Gonzalez, Omar H. Khalaf, Angela M. Arenas-Gamboa
Article
Microbiology
Martha E. Hensel, Daniel G. Garcia-Gonzalez, Sankar P. Chaki, Air Hatwig, Paul W. Gordy, Richard Bowen, Thomas A. Ficht, Angela M. Arenas-Gamboa
Article
Immunology
Martha E. Hensel, Sankar P. Chaki, Lauren Stranahan, Anthony E. Gregory, Erin J. van Schalk, Daniel G. Garcia-Gonzalez, Omar Khalaf, James E. Samuel, Angela M. Arenas-Gamboa
INFECTION AND IMMUNITY
(2020)
Article
Public, Environmental & Occupational Health
Benjamin W. Neuman, Wesley A. Brashear, Marcel Brun, Sankar P. Chaki, Rebecca S. B. Fischer, Sierra J. Guidry, Joshua E. Hill, Andrew E. Hillhouse, Charles D. Johnson, Melissa M. Kahl-McDonagh, Richard P. Metz, Allison C. Rice-Ficht, Jennifer A. Shuford, Tiffany A. Skaggs, Matthew A. Stull, David W. Threadgill, Yao Akpalu, Kurt Zuelke
Summary: The study identified three novel viral variants in a college campus population, indicating the potential of undervaccinated younger individuals as a source of spread and generation of new variants. Whole genome sequencing proved to be a valuable routine disease surveillance tool.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Correction
Immunology
Jamie S. Benn, Sankar P. Chaki, Yi Xu, Thomas A. Ficht, Allison C. Rice-Ficht, Walter E. Cook
Article
Microbiology
Sankar Prasad Chaki, Melissa M. Kahl-McDonagh, Benjamin W. Neuman, Kurt A. Zuelke
Summary: This study developed a rapid and cost-effective strategy for monitoring SARS-CoV-2 variants using spike protein receptor-binding-motif (RBM)-targeted Sanger sequencing in saliva and nasal swab samples.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Slim Zriba, Daniel G. Garcia-Gonzalez, Omar H. Khalaf, Lance Wheeler, Sankar P. Chaki, Allison Rice-Ficht, Thomas A. Ficht, Angela M. Arenas-Gamboa
Article
Microbiology
Aseem Pandey, Sheng Li Ding, Qing-Ming Qin, Rahul Gupta, Gabriel Gomez, Furong Lin, Xuehuan Feng, Luciana Fachini da Costa, Sankar P. Chaki, Madhu Katepalli, Elizabeth D. Case, Erin J. van Schaik, Tabasum Sidiq, Omar Khalaf, Angela Arenas, Koichi S. Kobayashi, James E. Samuel, Gonzalo M. Rivera, Robert C. Alaniz, Sing-Hoi Sze, Xiaoning Qian, William J. Brown, Allison Rice-Ficht, William K. Russell, Thomas A. Ficht, Paul de Figueiredo
CELL HOST & MICROBE
(2017)