Article
Engineering, Environmental
Guofang Xu, Ning Zhang, Xuejie Zhao, Chen Chen, Chunfang Zhang, Jianzhong He
Summary: This study explores the potential for offshore marine microbiota to transform organohalide pollutants and finds that marine Dehalococcoidia have a versatile dehalogenation capacity. The study demonstrates dechlorination of diverse organohalides by offshore marine microbiota, including polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA), and 2,4,6-trichlorophenol (2,4,6-TCP). It also identifies the presence of Dehalococcoides and uncultivated Dehalococcoidia in initial sediment microcosms, with Dehalococcoides being dominant in all enrichment cultures.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Xiuying Li, Yi Yang, Jingjing Wang, Huijuan Jin, Yaozhi Zhang, Yiru Cui, Yufang Song, Jun Yan
Summary: This study identified a anaerobic enrichment culture derived from contaminated river sediment that can mediate the reductive dechlorination of Diclofenac (DCF). It also discovered a new Dehalogenimonas population and highlighted the relevant roles of organohalide-respiring bacteria in the natural attenuation of halogenated contaminants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Martina Bertolini, Sarah Zecchin, Giovanni Pietro Beretta, Patrizia De Nisi, Laura Ferrari, Lucia Cavalca
Summary: The study evaluated a bioremediation approach for decontaminating an aquifer affected by organohalides from an industrial landfill. Results showed that the addition of a reducing substrate could stimulate natural organohalide respiration, leading to degradation of the contaminants. Field tests demonstrated a decrease in chloroethenes concentration, suggesting the potential for further improvement of the approach.
Article
Engineering, Environmental
Rifeng Wu, Rui Shen, Zhiwei Liang, Shengzhi Zheng, Yong Yang, Qihong Lu, Lorenz Adrian, Shanquan Wang
Summary: Strategies based on ecological principles can enhance the colonization of OHRB in niches, thereby improving the bioremediation efficiency of chloroethene-contaminated sites.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Bao-Anh Thi Nguyen, Ju-Liang Hsieh, Shou-Chen Lo, Sui-Yuan Wang, Chun-Hsiung Hung, Eugene Huang, Shih-Hsun Hung, Wei-Chih Chin, Chieh-Chen Huang
Summary: In this study, the endophytic bacterium Burkholderia cenocapacia 869T2 was found to degrade the most toxic dioxin congener TCDD by nearly 95% after one-week of an aerobic incubation, with a potential general dioxin degradation pathway proposed. The investigation also revealed the crucial role of a catabolic gene, L-2-haloacid dehalogenase (2-HAD), in dioxin dehalogenation in aerobic conditions. Transcriptional analysis indicated high gene expressions of catabolic genes involved in dioxin metabolism in the presence of TCDD.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Gao Chen, Fadime Kara Murdoch, Yongchao Xie, Robert W. Murdoch, Yiru Cui, Yi Yang, Jun Yan, Trent A. Key, Frank E. Loffler
Summary: Researchers discovered a new anaerobic bacterium that can efficiently dechlorinate vinyl chloride (VC) to ethene. The bacterium utilizes formate and hydrogen as electron donors for reductive dechlorination, providing new opportunities for effective remediation of VC contamination in groundwater.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Lisi Jiang, Yi Yang, Huijuan Jin, Hongyan Wang, Cynthia M. Swift, Yongchao Xie, Torsten Schubert, Frank E. Loffler, Jun Yan
Summary: Chlorinated ethanes can be transformed into harmless substances through the actions of specific microbial strains, such as Geobacter and Dehalococcoides. The presence of different strains in different reaction processes provides insights into the detoxification mechanism of organohalide compounds.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Review
Microbiology
Mohammad Sufian Bin Hudari, Hans Richnow, Carsten Vogt, Ivonne Nijenhuis
Summary: This article summarizes the current knowledge on reductive dehalogenation activities of chlorinated ethenes at different temperatures, including the activity and dehalogenation extent in laboratory isolates and enrichment cultures at different temperatures. It also discusses the indirect effects on biotransformation and the impact of anthropogenic activities related to thermal heat management on groundwater temperature.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Microbiology
Jessica M. Ewald, Jerald L. Schnoor, Timothy E. Mattes
Summary: By studying the microbial communities containing Dehalococcoides, this research reveals the differential abundance of functional genes and provides support for the bioremediation of halogenated organic contaminants.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Review
Engineering, Environmental
Rifeng Wu, Shanquan Wang
Summary: The integration of bioremediation with organohalide-respiring bacteria (OHRB) and sulfate radicals-based oxidation (Bio-RD-PAO) shows promising results in addressing organohalide pollution, particularly for lowly-halogenated organohalides. This approach offers advantages such as complete attenuation, high reactivity, and non-selectivity to organohalides, making it a potential solution for future remediation applications.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2022)
Article
Engineering, Environmental
Clara Reino, Chang Ding, Lorenz Adrian
Summary: Dehalococcoides mccartyi strain CBDB1, a strictly anaerobic organohalide-respiring bacterium, was successfully cultivated in a continuous stirring tank reactor (CSTR) using acetate, hydrogen, and the brominated amino acid D-or L-3,5-dibromotyrosine as substrates. The cultivation in the CSTR led to high cell densities and dominance of strain CBDB1, demonstrating its potential for biomass production and remediation of halogenated aromatic-contaminated environments. Proteomics analyses revealed the high expression of the reductive dehalogenase CbdbA238 with D-and L-3,5-dibromotyrosine, suggesting their promising role in biomass production.
Article
Microbiology
Huijuan Jin, Leitao Huo, Yi Yang, Yan Lv, Jingjing Wang, Julien Maillard, Christof Holliger, Frank E. Loffler, Jun Yan
Summary: Two anaerobic bacterial isolates, ACSDCET and ACSTCE, capable of respiring tetrachloroethene (PCE), were characterized. These strains showed unique morphological and physiological characteristics, including optimum growth conditions, electron donors, and acceptors. Genomic analysis further confirmed the distinction of these strains from other Sulfurospirillum species, proposing a new species named Sulfurospirillum diekertiae sp. nov. This study provides important insights into the diversity and functionalities of PCE-respiring bacteria.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Microbiology
Jesica M. Soder-Walz, Kenneth Wasmund, Darja Deobald, Teresa Vicent, Lorenz Adrian, Ernest Marco-Urrea
Summary: This study sequenced the genome of Dehalobacter sp. strain 8M and analyzed its protein expression. It discovered evidence of interactions among respiratory proteins and discussed implications for the respiratory architecture and advances in understanding this unique respiratory chain.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Masaki Asai, Naoko Yoshida, Toshiya Kusakabe, Mohamed Ismaeil, Takumi Nishiuchi, Arata Katayama
Summary: This study presents the isolation and identification of a novel strain of Dehalococcoides mccartyi, NIT01, which is capable of completely dechlorinating trichloroethene to ethene. The genomic analysis and characterization of RdhA enzymes provide insights into the molecular mechanism of dechlorination.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Juan Fausto Ortiz-Medina, Bilgen Yuncu, Lydia Ross, Brad Elkins
Summary: This article demonstrates the importance of pH adjustment and control in achieving complete enhanced reductive dechlorination (ERD) of chlorinated compounds. The study shows that through proper pH adjustment and the addition of colloidal buffers and nutrients, complete ERD can be achieved in challenging sites with low pH values and low buffering capacity.
INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT
(2023)
Article
Environmental Sciences
Wei-Ying Ouyang, Steffen Kuemmel, Lorenz Adrian, Yong-Guan Zhu, Hans H. Richnow
Summary: This study analyzed compound specific isotope fractionation of the antibiotic sulfamethoxazole (SMX) during anaerobic transformation using the model organism Desulfovibrio vulgaris Hildenborough. The results showed significant carbon and hydrogen isotopic fractionation due to the rearrangement of the isoxazole ring in SMX. Carbon isotope fractionation was higher than values reported for aerobic degradation or abiotic reactions, indicating its potential as a parameter to differentiate reaction mechanisms of SMX transformation. The observed apparent kinetic isotope effect for anaerobic transformation was distinct from that reported for microbial aerobic degradation. Dual-element (C-H) isotope analysis of SMX using gas chromatography was also performed in this study.
Article
Plant Sciences
Mahreen Yahya, Maria Rasul, Sayed Zajif Hussain, Adil Dilawar, Midrar Ullah, Lubna Rajput, Aftab Afzal, Muhammad Asif, Tesfaye Wubet, Sumera Yasmin
Summary: Climate change threatens wheat production, which may lead to a decline in crop production by 2030. Phosphorus (P) deficiency is also a major factor affecting yield. This study demonstrates the importance of an integrated approach to manage P nutrients and develop effective PSB consortia for wheat varieties, considering soil health and agro-climatic conditions. The results show that inoculated wheat plants with the designed consortia exhibited increased grain yield and seed P content.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Green & Sustainable Science & Technology
Margot Neyret, Sophie Peter, Gaetane Le Provost, Steffen Boch, Andrea Larissa Boesing, James M. Bullock, Norbert Hoelzel, Valentin H. Klaus, Till Kleinebecker, Jochen Krauss, Joerg Mueller, Sandra Mueller, Christian Ammer, Francois Buscot, Martin Ehbrecht, Markus Fischer, Kezia Goldmann, Kirsten Jung, Marion Mehring, Thomas Mueller, Swen C. Renner, Peter Schall, Michael Scherer-Lorenzen, Catrin Westphal, Tesfaye Wubet, Peter Manning
Summary: This study provides a tool to quantify the social impact of land-use changes by combining broad ecological data with information on the ecosystem service priorities of multiple stakeholder groups. The research shows that most major land-use changes proposed for Europe could lead to social conflicts and reduced multifunctionality. However, moderate gains in multifunctionality and equity can be achieved by expanding and diversifying forests and de-intensifying grasslands.
NATURE SUSTAINABILITY
(2023)
Article
Biotechnology & Applied Microbiology
Emea Okorafor Ude, Jucelaine Haas, Mohammed Kaysar Kaiyoum, Chang Ding, Lorenz Adrian
Summary: The effect of environmental stresses on anammox bacteria was investigated using an overnight batch activity test. It was found that oxygen strongly inhibited anammox activity, but this inhibition could be reversed by sodium sulfite. Additionally, antibiotics, activated carbon, and Fe2O3 had positive effects on anammox activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biodiversity Conservation
Andreas Schuldt, Xiaojuan Liu, Francois Buscot, Helge Bruelheide, Alexandra Erfmeier, Jin-Sheng He, Alexandra-Maria Klein, Keping Ma, Michael Scherer-Lorenzen, Bernhard Schmid, Thomas Scholten, Zhiyao Tang, Stefan Trogisch, Christian Wirth, Tesfaye Wubet, Michael Staab
Summary: Carbon-focused climate mitigation strategies are important in forests, but we need better understanding of their impact on biodiversity. A study using a large dataset from subtropical forests found that aboveground carbon was not strongly related to multitrophic diversity, while total carbon including belowground carbon was a significant predictor. Relationships were nonlinear and strongest for lower trophic levels. Tree species richness and stand age influenced these relationships, suggesting long-term forest regeneration is effective in reconciling carbon and biodiversity targets. This highlights the need to carefully evaluate the biodiversity benefits of climate-oriented management.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Plant Sciences
Lioba Rueger, Minh Ganther, Jule Freudenthal, Jan Jansa, Anna Heintz-Buschart, Mika Tapio Tarkka, Michael Bonkowski
Summary: Plants have a significant impact on the development of microbial communities in their rhizosphere, and the role of root caps and specific root zones in microbial community assembly is still unclear. In this study, the composition of prokaryote and protist microbiomes along maize roots was compared between intact and decapped roots, as well as between wild type and root hairless mutant. The absence of root caps had a stronger effect on microbiome composition than the absence of root hairs, affecting microbial community composition at older root zones and higher trophic levels.
Article
Microbiology
Bala Singavarapu, Jianqing Du, Remy Beugnon, Simone Cesarz, Nico Eisenhauer, Kai Xue, Yanfen Wang, Helge Bruelheide, Tesfaye Wubet
Summary: Loss of multifunctional microbial communities can negatively affect ecosystem services, especially forest soil nutrient cycling. Therefore, exploration of the genomic potential of soil microbial communities, particularly their constituting subcommunities and taxa for nutrient cycling, is vital to get an in-depth mechanistic understanding for better management of forest soil ecosystems.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jesica M. Soder-Walz, Kenneth Wasmund, Darja Deobald, Teresa Vicent, Lorenz Adrian, Ernest Marco-Urrea
Summary: This study sequenced the genome of Dehalobacter sp. strain 8M and analyzed its protein expression. It discovered evidence of interactions among respiratory proteins and discussed implications for the respiratory architecture and advances in understanding this unique respiratory chain.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Shangwei Zhang, Wu Wen, Xinghui Xia, Wei Ouyang, Bi-xian Mai, Lorenz Adrian, Gerrit Schuurmann
Summary: Thermodynamics reveals a proton-coupled two-electron transfer mechanism in microbial B-12-dependent reductive dehalogenation of aromatics, enhancing our understanding of the dehalogenation process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Rifeng Wu, Rui Shen, Zhiwei Liang, Shengzhi Zheng, Yong Yang, Qihong Lu, Lorenz Adrian, Shanquan Wang
Summary: Strategies based on ecological principles can enhance the colonization of OHRB in niches, thereby improving the bioremediation efficiency of chloroethene-contaminated sites.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Forestry
Pascal Edelmann, Wolfgang W. Weisser, Didem Ambarli, Claus Baessler, Francois Buscot, Martin Hofrichter, Bjorn Hoppe, Harald Kellner, Cynthia Minnich, Julia Moll, Derek Persoh, Sebastian Seibold, Claudia Seilwinder, Ernst-Detlef Schulze, Stephan Woellauer, Werner Borken
Summary: A long-term experiment in Germany suggests that climate, soil traits, and forest structure have significant impacts on the decay process and mass loss of deadwood. Soil nutrient content and precipitation have negative effects on mass loss, while temperature has a positive effect. Forest structure has a small influence on mass loss. The results indicate that at the regional scale, organismic diversity and microbial activity have a stronger impact on the decay process than exogenous factors.
FOREST ECOLOGY AND MANAGEMENT
(2023)
Article
Ecology
Qicheng Bei, Thomas Reitz, Beatrix Schnabel, Nico Eisenhauer, Martin Schaedler, Francois Buscot, Anna Heintz-Buschart
Summary: The study investigates the effects of extreme summers and future climate scenarios on soil microbiomes. Extreme heatwaves and droughts in Central Europe during 2018-2019 significantly impacted the structure and function of soil microbiomes. Actinobacteria showed a competitive advantage in extreme summers due to the biosynthesis of geosmin and 2-methylisoborneol.
Article
Microbiology
Maria L. Bonatelli, Thore Rohwerder, Denny Popp, Yu Liu, Caglar Akay, Carolyn Schultz, Kuan-Po Liao, Chang Ding, Thorsten Reemtsma, Lorenz Adrian, Sabine Kleinsteuber
Summary: In this study, the metabolism of acesulfame by microbial degraders was investigated, leading to the identification of two gene clusters involved in the degradation pathway. Transposition of these gene clusters between chromosome and plasmid was observed. The global evolution and spread of the acesulfame degradation pathway was also elucidated, highlighting the importance of this research in understanding microbial degradation of harmful substances and environmental remediation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Kenneth Wasmund, Alba Trueba-Santiso, Teresa Vicent, Lorenz Adrian, Stephane Vuilleumier, Ernest Marco-Urrea
Summary: In this study, a novel DCM-degrading strain was obtained from a stable DCM-degrading consortium, and their proteome analysis revealed the significant role of mec-associated methyltransferases in anaerobic DCM metabolism.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Physical
Shangwei Zhang, Wei Ouyang, Xinghui Xia, Wu Wen, Lorenz Adrian, Gerrit Schueuermann
Summary: Microbial reductive dechlorination is an environmentally friendly and highly desirable method for addressing PCB pollution in soil, sediment, and underground water. The reaction is catalyzed by supernucleophilic cob(i)alamin housed in reductive dehalogenases (RDases), but the mechanism is still unknown.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
