Article
Immunology
Yufang Guo, Hongming Xia, Tingting Dai, Tingli Liu
Summary: A detection assay using recombinase polymerase amplification (RPA) combined with CRISPR/Cas12a system was developed for Phytophthora sojae. The method was highly specific and sensitive, and showed potential for monitoring soybean root rot in the field.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Shun Zhou, Qing Huang, Mingming Yu, Ying Li, Xujia Zheng, Yunji Xiu
Summary: The study developed a rapid detection method, LAMP-HNB assay, for Aeromonas salmonicida, an important pathogen in fish farming, with good specificity and high sensitivity. The method has potential applications in diagnostic testing, health certification, and surveillance programs.
JOURNAL OF FISH DISEASES
(2021)
Article
Biochemical Research Methods
Xuliang Zhang, Yingfeng Zhao, Chang Ma, Wei Wu, Min Dong, Jinwei You, Jie Liu, Shifeng Yun
Summary: In this study, a rapid and visual detection method for minute virus of mice (MVM) using loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye was developed. The assay showed high sensitivity and specificity, with a lower limit of detection compared to conventional PCR. The established LAMP system was successfully applied to detect MVM infections in laboratory animal samples.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Infectious Diseases
Sayamon Hongjaisee, Natteewan Doungjinda, Woottichai Khamduang, Tanawan Samleerat Carraway, Jiraprapa Wipasa, Jose D. Debes, Khuanchai Supparatpinyo
Summary: The study developed a reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for detecting hepatitis C virus (HCV) in a single closed tube. The assay showed high sensitivity, specificity, and stability in detecting different HCV genotypes, making it a potentially useful tool for identifying individuals in need of HCV treatment, particularly in resource-limited settings.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Ilada Choopara, Yothin Teethaisong, Narong Arunrut, Sudaluck Thunyaharn, Wansika Kiatpathomchai, Naraporn Somboonna
Summary: The study developed a simple ITS1 LAMP-HNB method for detection of fungi, demonstrating high sensitivity and applicability for preventing opportunistic fungal infections in resource-constrained areas.
Article
Microbiology
Dominika G. Siegieda, Jacek Panek, Magdalena Frac
Summary: This study demonstrates a rapid and effective detection method for Phytophthora spp. and Phytophthora cactorum using Loop-Mediated Isothermal Amplification (LAMP), which is simpler and faster compared to traditional culture-based methods and costly molecular techniques.
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Plant Sciences
R. Logeshwari, C. Gopalakrishnan, A. Kamalakannan, J. Ramalingam, R. Saraswathi
Summary: A rapid and highly specific detection method based on LAMP technology was developed in this study for the seed-borne pathogen Sarocladium oryzae. This method offers quick and reliable detection of the pathogen, which is important for maintaining the health of rice seeds.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Sandra V. Gomez-Gutierrez, Stephen B. Goodwin
Summary: LAMP is a molecular technique for detection of plant pathogens, known for its specificity, sensitivity, and ability to conduct reactions at a constant temperature. This review explains the amplification process, visualization techniques, advantages, and disadvantages of LAMP compared to alternative isothermal approaches. It also presents the application of LAMP in detecting fungal pathogens and viruses in wheat, as well as the development of real-time and multiplex LAMP assays.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Zhichao Zhang, Long Lin, Han Chen, Wenwu Ye, Suomeng Dong, Xiaobo Zheng, Yuanchao Wang
Summary: In this study, open chromatin and cis-regulatory elements were identified in the oomycete plant pathogen Phytophthora sojae using ATAC-seq. The nucleosome-free regions were enriched in gene-promoter regions and associated with 40% of P. sojae genes. Chromatin accessibility was positively correlated with gene transcription level. Motif discovery based on nucleosome-free region peaks identified several candidate cis-regulatory motifs, some of which were associated with putative pathogenicity-related genes.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Letter
Biochemistry & Molecular Biology
Ruixiang Cheng, Ruoxin Mei, Rong Yan, Hongyu Chen, Dan Miao, Lina Cai, Jiayi Fan, Gairu LI, Ran Xu, Weiguo Ye, Yu Gao, Wenwu Ye, Shuo Su, Tianfu Han, Junyi Gai, Yuanchao Wang, Xiaorong Tao, Yi Xu
Review
Microbiology
Yan Wang, Rory N. Pruitt, Thorsten Nuernberger, Yuanchao Wang
Summary: Plant pathogens utilize various strategies to evade plant immunity, hindering the effective use of host genetic resistance for disease control. This Review discusses current knowledge on how microbial pathogens successfully infect plants by evading their immune responses and explores the potential for engineering crop resistance based on this knowledge.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Plant Sciences
Xiaolin Zhao, Kainan Li, Sujiao Zheng, Jin Yang, Changjun Chen, Xiaobo Zheng, Yuanchao Wang, Wenwu Ye
Summary: This study identified Diaporthe species associated with soybean stem blight in China through field surveys and experiments. The results showed that Diaporthe genus is an important pathogen causing significant yield loss in soybean production in China. Variations in pathogenicity and genetic diversity were observed among different species. These findings are important for developing effective management strategies against soybean stem blight.
Article
Microbiology
Hongjun Yang, Yao Zhao, Jiaxin Ma, Zhenyang Rong, Jiajia Chen, Yuanchao Wang, Xiaobo Zheng, Wenwu Ye
Summary: Wheat straw return has diverse effects on the microbial community composition of soybean roots, with a greater impact on fungal communities and significant changes in bulk soil, rhizosphere, and rhizoplane.
Article
Biochemistry & Molecular Biology
Lan Wang, Hanmei Liu, Mingmei Zhang, Yu Ye, Lei Wang, Jinyi Zhu, Zhaodan Chen, Xiaobo Zheng, Yan Wang, Yuanchao Wang
Summary: Plant pattern recognition receptors (PRRs) are receptors that sense microbial invasion and activate immune responses. This study identified a glycoside hydrolase family 12 (GH12) protein Ps109281 secreted by Phytophthora sojae during infection. Ps109281 promotes P. sojae infection but does not induce cell death. It triggers immune responses by interacting with the receptor-like protein RXEG1. The loss of cell death induction is linked to a sequence polymorphism at the N-terminus.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Baiyu Zhang, Zhichao Zhang, Saijiang Yong, Shuyang Yu, Hui Feng, Maozhu Yin, Wenwu Ye, Yuanchao Wang, Min Qiu
Summary: This study identifies a leucine-rich repeat (LRR) protein that plays a crucial role in the development of Phytophthora sojae zoospores. Its absence leads to reduced mobility and chemotaxis of zoospores, as well as decreased cyst germination and virulence.
Article
Biochemistry & Molecular Biology
Yao Zhao, Bo Yang, Huawei Xu, Jinbin Wu, Zhiyang Xu, Yuanchao Wang
Summary: The oomycete pathogen Phytophthora sojae secretes RXLR effectors to suppress host immune responses, promoting infection. The virulence effector Avh94 stabilizes the repressor JAZ1/2 of the jasmonic acid (JA) signaling pathway to inhibit JA signaling, thereby enhancing infection.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xufang Qiu, Liang Kong, Han Chen, Yachun Lin, Siqun Tu, Lei Wang, Zhiyuan Chen, Mengzhu Zeng, Junhua Xiao, Peiguo Yuan, Min Qiu, Yan Wang, Wenwu Ye, Kaixuan Duan, Suomeng Dong, Yuanchao Wang
Summary: This study reveals that the nuclear effector PsAvh110 from the pathogen Phytophthora sojae suppresses plant immunity by disrupting the formation of the GmLHP1-2/GmPHD6 complex, leading to reduced expression of immunity genes. PsAvh110 also represses the expression of immune-associated genes through binding to G-rich elements in gene promoters. The findings suggest a regulatory mechanism by which pathogen effectors target a transcriptional complex to manipulate plant immunity.
Article
Soil Science
Qi Yang, Xueling Yang, Xiaowei Huang, Wenwu Ye, Tianyu Wang, Zhongyi Cheng, Jiachun Shi, Yong Li, Jianming Xu, Yan He
Summary: While seed coating with fungicide is commonly used in soybean culture to control diseases, its impact on root-associated microbial communities remains understudied. This study found that fungicide seed coating had a greater effect on endophytic communities, including root endosphere and nodules, than rhizosphere communities. Many beneficial microbes responded positively to fungicide seed coating. Seed coating also increased the abundance of nitrogen fixation genes. Root-associated microbes with coatings had a higher complexity of microbial interactions. Fungicide seed coating did not show any negative impact on the root-associated microecology of mature soybean and may have a lower environmental risk than expected.
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Zhaodan Chen, Fan Liu, Mengzhu Zeng, Lei Wang, Hanmei Liu, Yujing Sun, Lan Wang, Zhichao Zhang, Zhiyuan Chen, Yuanpeng Xu, Mingmei Zhang, Yeqiang Xia, Wenwu Ye, Suomeng Dong, Francine Govers, Yan Wang, Yuanchao Wang
Summary: In this study, scientists identified a receptor named REL that can recognize the extracellular protein ELicitin. REL is a receptor-like protein (RLP) that mediates plant resistance against Phytophthora by binding to ELicitin. The island domain (ID) within the leucine-rich repeat (LRR) domain of REL was found to be crucial for ELicitin recognition.
Article
Plant Sciences
Han Chen, Yujie Fang, Wenrui Song, Haidong Shu, Xi Li, Wenwu Ye, Yuanchao Wang, Suomeng Dong
Summary: In this study, a highly induced PsKMT3 gene was found to play an important role in regulating reproductive development and pathogenicity in Phytophthora sojae. The deletion of PsKMT3 resulted in asexual development and pathogenicity defects. The study also revealed that histone H3K36 trimethylation was significantly reduced and involved in the activation of RxLR effector genes.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Shuchen Wang, Sen Yang, Kaixin Dai, Wenyue Zheng, Xiaoyi Zhang, Bo Yang, Wenwu Ye, Xiaobo Zheng, Yuanchao Wang
Summary: In this study, a putative effector protein Fg62 was identified from the secretome of the plant pathogenic fungus Fusarium graminearum. Fg62 modulates host immunity and facilitates infection by upregulating during early stages of infection and inducing plant cell death. It also activates plant immunity and enhances plant resistance to various pathogens.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Lin Liu, Yeqiang Xia, Yingchen Li, Yong Zhou, Xiaofeng Su, Xiaojing Yan, Yan Wang, Wende Liu, Hongmei Cheng, Yuanchao Wang, Qing Yang
Summary: Phytopathogenic fungi secrete chitin deacetylase (CDA) to evade the host's immune defense, and the authors identify common structural features of phytopathogenic fungal CDAs and find potential CDA inhibitors for controlling plant diseases.
NATURE COMMUNICATIONS
(2023)
