期刊
FEMS MICROBIOLOGY LETTERS
卷 335, 期 2, 页码 95-103出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1574-6968.2012.02641.x
关键词
genome mining; pathway mutagenesis; siderophore chemistry
类别
资金
- NIAID NIH HHS [R01 AI095125, AI095125] Funding Source: Medline
- NIGMS NIH HHS [GM085770, R01 GM085770, GM08283, T32 GM008283] Funding Source: Medline
Many bacteria produce siderophores for sequestration of growth-essential iron. Analysis of the Salinispora genomes suggests that these marine actinomycetes support multiple hydroxamate- and phenolate-type siderophore pathways. We isolated and characterized desferrioxamines (DFOs) B and E from all three recognized Salinispora species and linked their biosyntheses in S.similar to tropica CNB-440 and S.similar to arenicola CNS-205 to the des locus through PCR-directed mutagenesis. Gene inactivation of the predicted iron-chelator biosynthetic loci sid2-4 did not abolish siderophore chemistry. Additionally, these pathways could not restore the native growth characteristics of the des mutants in iron-limited media, although differential iron-dependent regulation was observed for the yersiniabactin-like sid2 pathway. Consequently, this study indicates that DFOs are the primary siderophores in laboratory cultures of Salinispora.
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