期刊
FEMS MICROBIOLOGY LETTERS
卷 313, 期 1, 页码 75-80出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2010.02124.x
关键词
copper; peroxide; Xanthomonas; ahpC
类别
资金
- National Centre for Genetic Engineering and Biotechnology [BTB-01-PG-14-5112]
- Chulabhorn Research Institute
- Mahidol University
- Chulabhorn Graduate Institute
Copper (Cu)-based biocides are important chemical controls for both fungal and bacterial diseases in crop fields. Here, we showed that Cu ions at a concentration of 100 mu M enhanced t-butyl hydroperoxide (tBOOH) and hydrogen peroxide (H2O2) killing of Xanthomonas campestris pv. campestris through different mechanisms. The addition of an antilipid peroxidation agent (alpha-tocopherol) and hydroxyl radical scavengers (glycerol and dimethyl sulphoxide) partially protected the bacteria from the Cu-enhanced tBOOH and H2O2 killing, respectively. Inactivation of the alkyl hydroperoxide reductase gene rendered the mutant vulnerable to lethal doses of copper sulphate, which could be alleviated by the addition of an H2O2 scavenger (pyruvate) and alpha-tocopherol. Taken together, the data suggest that Cu ions influence the killing effect of tBOOH through the stimulation of lipid peroxidation, while hydroxyl radical production is the underlying mechanism responsible for the Cu-ion-enhanced H2O2 killing effects.
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