期刊
FEMS MICROBIOLOGY LETTERS
卷 312, 期 1, 页码 84-92出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1574-6968.2010.02105.x
关键词
TOL plasmid; biofilm; extracellular DNA; air-liquid interface
类别
资金
- EC [MEXT-CT-2005-024004]
- Villum Kan Rasmussen Center for Environmental and Agricultural Microbiology
Adherent growth of Pseudomonas putida KT2440 with and without the TOL plasmid (pWWO) at the solid-liquid and air-liquid interface was examined. We compared biofilm formation on glass in flow cells, and assayed pellicle (air-liquid interface biofilm) formation in stagnant liquid cultures by confocal laser scanning microscopy. The TOL-carrying strains formed pellicles and thick biofilms, whereas the same strains without the plasmid displayed little adherent growth. Microscopy using fluorescent nucleic acid-specific stains revealed differences in the production of extracellular polymeric substances: TOL carriage leads to more extracellular DNA (eDNA) in pellicles and biofilms. Pellicles were dissolved by DNase I treatment. Enhanced cell lysis due to plasmid carriage was ruled out as the mechanism for eDNA release. We report, for the first time, that carriage of a conjugative plasmid leads to increased biofilm formation by production of eDNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据