期刊
FEMS MICROBIOLOGY LETTERS
卷 305, 期 1, 页码 14-17出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2010.01891.x
关键词
cloning and sequencing; insertion; protoplast-derived monokaryons
类别
资金
- Research & Development Special Fund for Public Welfare Industry [3-27]
To examine why we failed in direct sequencing of rRNA gene internal transcribed spacer (ITS) in Pleurotus nebrodensis, we obtained monokaryons of P. nebrodensis (00489 and 00491) using a protoplast monokaryonization technique. PCR products of ITS amplifications were sequenced. There was a base pair insertion/deletion difference between the two nuclei of P. nebrodensis that led to failure in direct sequencing.
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