期刊
FEMS MICROBIOLOGY LETTERS
卷 302, 期 1, 页码 22-31出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01837.x
关键词
PhoB; biofilm; Vibrio cholerae
类别
资金
- National Institute of General Medical Sciences [GM008248]
- National Institute of Allergy and Infectious Disease [AI63187]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI063187] Funding Source: NIH RePORTER
The PhoBR regulatory system is required for the induction of multiple genes under conditions of phosphate limitation. Here, we examine the role of PhoB in biofilm formation and environmental stress response in Vibrio cholerae of the El Tor biotype. Deletion of phoB or hapR enhanced biofilm formation in a phosphate-limited medium. Planktonic and redispersed biofilm cells of the delta phoB mutant did not differ from wild type for the expression of HapR, suggesting that PhoB negatively affects biofilm formation through an HapR-independent pathway. The delta phoB mutant exhibited elevated expression of exopolysaccharide genes vpsA and vpsL compared with the wild type. Deletion of hapR enhanced the expression of the positive regulator vpsT, but had no effect on the expression of vpsR. In contrast, deletion of phoB enhanced the expression of the positive regulator vpsR, but had no effect on the expression of hapR and vpsT. The delta phoB mutant was more sensitive to hydrogen peroxide compared with the wild type and with an isogenic delta rpoS mutant. Conversely, the delta phoB mutant was more resistant to acidic conditions and high osmolarity compared with the wild type and with an isogenic delta rpoS mutant. Taken together, our data suggest that phosphate limitation induces V. cholerae to adopt a free-swimming life style in which PhoB modulates environmental stress response in a manner that differs from the general stress response regulator RpoS.
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