期刊
FEMS MICROBIOLOGY LETTERS
卷 300, 期 2, 页码 237-241出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01802.x
关键词
C-1027; enediyne; flavin reductase; halogenase; monooxygenase; SgcE6
类别
资金
- NIH [CA78747, CA113297, CA1059845]
- NSERC
The C-1027 enediyne antitumor antibiotic from Streptomyces globisporus possesses an (S)-3-chloro-5-hydroxy-beta-tyrosine moiety, the chloro- and hydroxy-substituents of which are installed by a flavin-dependent halogenase SgcC3 and monooxygenase SgcC, respectively. Interestingly, a single flavin reductase, SgcE6, can provide reduced flavin to both enzymes. Bioinformatics analysis reveals that, similar to other flavin reductases involved in natural product biosynthesis, SgcE6 belongs to the HpaC-like subfamily of the Class I flavin reductases. The present study describes the steady-state kinetic characterization of SgcE6 as a strictly NADH- and FAD-specific enzyme.
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