期刊
FEMS MICROBIOLOGY LETTERS
卷 297, 期 2, 页码 250-254出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1574-6968.2009.01689.x
关键词
transposon; tryptophan auxotrophs; archaea; methanogens
类别
资金
- NIH
Methanococcus maripaludis is an anaerobic, methane-producing archaeon that utilizes H(2) or formate for the reduction of CO(2) to methane. Tryptophan auxotrophs were constructed by in vitro insertions of the Tn5 transposon into the tryptophan operon, followed by transformation into M. maripaludis. This method could serve for rapid insertions into large cloned DNA regions.
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