期刊
FEMS MICROBIOLOGY LETTERS
卷 293, 期 1, 页码 122-129出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01516.x
关键词
Agrobacterium tumefaciens; copper; cytochrome c oxidase; oxidative stress; Sco
类别
资金
- National Center for Genetic Engineering and Biotechnology [BT-B-01-PG-14-5112]
- Mahidol University
- Center of Excellence on Environmental Health, Toxicology and Management of Chemicals (ETM)
- Thailand Research Fund [PHD/0086/2546]
Sco (for the synthesis of cytochrome c oxidase) is a mitochondrial membrane protein essential for the correct assembly of cytochrome c oxidase. sco homolog genes exist in a wide variety of bacterial species. Inactivation of Agrobacterium tumefaciens sco leads to markedly decreased cytochrome c oxidase activity. This phenotype can be complemented by either supplementing the culture medium with copper or by a plasmid containing sco. The sco mutant also alters resistance to a superoxide generator menadione and H2O2. Mutational analysis of conserved cysteine residues and a histidine residue within the putative copper ions binding motif of Sco indicates that these residues are essential for the biological activity of Sco. To summarize, we find that A. tumefaciens sco is required for the delivery of copper into active cytochrome c oxidase and in maintaining optimal resistance levels to oxidative stress.
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