Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Microbiology
Eric Cheng, Dorjbal Dorjsuren, Stephanie Lehman, Charles L. Larson, Steven A. Titus, Hongmao Sun, Alexey Zakharov, Ganesha Rai, Robert A. Heinzen, Anton Simeonov, Matthias P. Machner
Summary: The bacterial type IV secretion system (T4SS) is an important machine for delivering effector proteins into host cells, and this process can be targeted by drugs. In this study, a high-throughput screening approach was used to identify compounds that interfere with T4SS function. Several compounds were found to efficiently interfere with processes dependent on a functional T4SS. These compounds also attenuated effector delivery by a related T4SS from another pathogen without impacting its growth in culture medium.
Article
Immunology
Donghyun Park, Samuel Steiner, Meng Shao, Craig R. Roy, Jun Liu
Summary: This study reveals that the different developmental stages of Coxiella burnetii affect the assembly and function of the Dot/Icm T4SS. The assembly of the Dot/Icm machine is associated with the transition of C. burnetii from SCV to LCV.
INFECTION AND IMMUNITY
(2022)
Review
Microbiology
Daniel C. Lockwood, Himani Amin, Tiago R. D. Costa, Gunnar N. Schroeder
Summary: To gain an advantage in interacting with eukaryotic hosts, many bacterial pathogens use protein secretion systems to release virulence factors. Legionella pneumophila's Dot/Icm T4BSS is an example of a complex and sophisticated secretion system that allows the bacteria to evade degradation and replicate within host cells. Recent advancements in imaging large protein complexes have provided new insights into the structure and transport mechanism of the T4BSS, as well as the functions of L. pneumophila effectors.
Review
Immunology
Nadja Blasey, Daria Rehrmann, Anna Katharina Riebisch, Sabrina Muehlen
Summary: Infections caused by Gram-negative pathogens pose a major health burden, and both respiratory and gastrointestinal infections are commonly associated with these pathogens. There has been an increase in antimicrobial resistance over the last decades, and bacterial infections may soon become a threat again. Gram-negative pathogens use virulence-associated secretion systems to inject effector proteins into host cells, facilitating their replication and survival. Inhibitors of these secretion systems have potential as therapeutics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Fisheries
Tao Liu, Zheng-Yang Zhou, Chen-wang Lu, Shang-Jie Bai, Jun Wang, Gao-Xue Wang
Summary: In this study, the researchers found that the T4SS of Yersinia ruckeri SC09 mediated bacterial virulence and immune evasion, possibly through the secretion of molecules such as STIR-1, STIR-2, and STIR-3. These findings contribute to a better understanding of the general rules of bacterial pathogenicity.
Article
Immunology
Sabine Drehkopf, Felix Scheibner, Daniela Buettner
Summary: In this study, it was found that X. euvesicatoria possesses two T4S systems, but neither of them is essential for its interaction with host plants or contact-dependent lysis of other bacteria. The chromosomal T4S gene cluster is incomplete, but the plasmid-localized vir gene cluster encodes a functional VirB/VirD4 T4S system involved in plasmid transfer. Interestingly, the targeting of T4S substrates from X. euvesicatoria to both VirB/VirD4 and Icm/Dot T4S systems may involve additional unknown mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Praneet Wangthaisong, Pongdet Piromyou, Pongpan Songwattana, Jenjira Wongdee, Kamonluck Teamtaisong, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong
Summary: SUTN9-2 is a broad host range strain capable of symbiosis with several legumes. It contains two copies of T4SS gene, homologous with the tra/trb operons, and the phylogenetic analysis reveals the horizontal transfer of T4SS among bradyrhizobia and mesorhizobia. Deletion and complementation experiments indicate that T4SS(1) positively affects nodulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Immunology
Tomoe Kitao, Tomoko Kubori, Hiroki Nagai
Summary: This review summarizes recent advances in the structural studies of the L. pneumophila IVB secretion system and provides insights into substrate recognition and transport mechanisms.
MICROBIOLOGY AND IMMUNOLOGY
(2022)
Article
Immunology
Ines P. Monteiro, Sofia Sousa, Vitor Borges, Paulo Goncalves, Joao Paulo Gomes, Luis Jaime Mota, Irina S. Franco
Summary: Two novel effectors, VFX05045 and VFX10045, of Legionella pneumophila were identified and their subcellular localizations were determined. Furthermore, the essential residues for nuclear targeting of VFX10045 were identified.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Samuel Steiner, Amit Meir, Craig R. Roy
Summary: The Cbu1754 protein, related to LvgA, plays a crucial role in the formation of CCV and intracellular replication of C. burnetii, although it is not a Dot/Icm-translocated effector protein. It interacts with IcmS and IcmW to form a chaperone complex, similar to the mechanism of the LvgA, IcmS, and IcmW proteins in Legionella pneumophila, indicating its importance in regulating replication mechanisms.
CELLULAR MICROBIOLOGY
(2021)
Article
Biology
Michael J. Sheedlo, Clarissa L. Durie, Jeong Min Chung, Louise Chang, Jacquelyn Roberts, Michele Swanson, Dana Borden Lacy, Melanie D. Ohi
Summary: This passage discusses the relationship between Legionella pneumophila and Legionnaires' disease, as well as the structure of the Dot/Icm T4SS core complex determined by single particle cryo-EM. It identifies new proteins contributing to the core T4SS structure and defines two areas of symmetry mismatch.
Review
Immunology
Mackenzie E. Ryan, Prashant P. Damke, Carrie L. Shaffer
Summary: The T4SS nanomachine plays a crucial role in bacterial pathogenesis and antibiotic resistance propagation. It enables the delivery of effector proteins, DNA export, and uptake, and in rare cases, facilitates transkingdom DNA translocation. Recent advances have uncovered new mechanisms underlying DNA translocation through T4SS machineries, highlighting functional plasticity and evolutionary adaptations that enable novel capabilities. This review discusses the molecular mechanisms, architectural features, and substrate recruitment strategies that contribute to T4SS functional diversity.
INFECTION AND IMMUNITY
(2023)
Review
Cell Biology
Flavia Viana, Shruthi Sachidanandan Peringathara, Arshad Rizvi, Gunnar N. Schroeder
Summary: Proteases are powerful enzymes that irreversibly cleave peptide bonds to control critical cell fate decisions. Bacterial pathogens deliver protease effectors through secretion systems to manipulate host processes, with functional diversity reflecting adaptations to specific hosts. Emerging concepts like effector-triggered immunity provide insights into host defense against protease attacks.
CELLULAR MICROBIOLOGY
(2021)
Article
Microbiology
Dehao Wang, Liying Wang, Dexi Bi, Jipeng Song, Guohao Wang, Ye Gao, Kathy F. J. Tang, Fanzeng Meng, Jingmei Xie, Fan Zhang, Jie Huang, Jianliang Li, Xuan Dong
Summary: In this study, we discovered a novel T4SS that mediates the transfer of VA1-type plasmids and found that the transfer is regulated by bacterial density, temperature, and nutrient levels.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
J. A. Aguilera Sammaritano, C. C. Lopez Lastra, A. Leclerque, F. Vazquez, M. E. Toro, C. P. D'Alessandro, A. G. S. Cuthbertson, B. E. Lechner
BIOCONTROL SCIENCE AND TECHNOLOGY
(2016)
Article
Entomology
Romina G. Manfrino, Christina Schuster, Katharina Saar, Claudia C. Lopez Lastra, Andreas Leclerque
JOURNAL OF APPLIED ENTOMOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
H. Gharsallah, I. Ksentini, N. Abdelhedi, S. Naayma, K. Hadj Taieb, M. Sahnoun, M. A. Triki, M. Ksantini, A. Leclerque
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Mycology
Alejandra C. Gutierrez, Andreas Leclerque, Romina G. Manfrino, Christian Luz, Walter A. O. Ferrari, Jorge Barneche, Juan J. Garcia, Claudia C. Lopez Lastra
Article
Biotechnology & Applied Microbiology
K. Hadj Taieb, H. Gharsallah, I Ksentini, C. Schuster, M. Fernandez-Bravo, I Garrido-Jurado, E. Quesada-Moragat, A. Leclerque, M. A. Triki, M. Ksantini
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Agronomy
Tinatin Doolotkeldieva, Saikal Bobushova, Christina Schuster, Mahabat Konurbaeva, Andreas Leclerque
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2019)
Article
Multidisciplinary Sciences
Ines Ksentini, Houda Gharsallah, Maryam Sahnoun, Christina Schuster, Sirine Hamli Amri, Rim Gargouri, Mohamed Ali Triki, Mohieddine Ksantini, Andreas Leclerque
Article
Biotechnology & Applied Microbiology
K. Hadj Taieb, H. Gharsallah, I. Ksentini, A. Leclerque, C. Schuster, M. Cheffi, S. Naayma, M. A. Triki, M. Ksantini
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Mycology
Alejandra C. Gutierrez, Eliana Ordoqui, Andreas Leclerque, Claudia Lopez Lastra
Article
Agronomy
Christoph S. Schmidt, Andreas Leclerque, Tobias Pfeiffer, Johannes W. Goessling, Marc Orlik, Behzad Jamshidi, Katharina Saar, Joerg Sellmann, Isabella Siepe, Eckhard Koch
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2020)
Article
Microbiology
Houda Gharsallah, Ines Ksentini, Sourour Naayma, Karama Hadj Taieb, Nour Abdelhedi, Christina Schuster, Mohamed Ali Triki, Mohieddine Ksantini, Andreas Leclerque
Article
Parasitology
Tinatin Doolotkeldieva, Saikal Bobushova, Ayperi Muratbekova, Christina Schuster, Andreas Leclerque
Summary: The study aimed to evaluate the potential of predatory fungi as biological control agents against parasitic plant nematodes, isolating and identifying highly active strains through in vitro and in vivo screening. Preliminary findings indicated that Arthrobotrys oligospora showed promising efficacy in immobilizing garlic stem nematodes, highlighting its potential as a nematicidal agent.
ACTA PARASITOLOGICA
(2021)
Article
Multidisciplinary Sciences
Haifa Ben Gharsa, Meriam Bouri, Amira Mougou Hamdane, Christina Schuster, Andreas Leclerque, Ali Rhouma
Summary: The study identifies a potential biological agent, B. velezensis MBY2, that can effectively suppress pathogenic Agrobacterium in plants, significantly reducing pathogen populations in maize and tomato plants. Through experiments, the strain demonstrates antagonistic effects in vitro and in vivo, effectively reducing gall development induced by the pathogen.
Article
Biotechnology & Applied Microbiology
Giuseppe Andolfo, Christina Schuster, Haifa Ben Gharsa, Michelina Ruocco, Andreas Leclerque
Summary: The newly reported genome sequence of P. vermicola DSM_17385 will serve as a well-supported reference for comparative genomics studies on Providencia bacteria. The presence of potential antibiotic resistance-associated genes in the P. vermicola DSM_17385 genome is noted, although unlike clinical Providencia strains, no plasmids carrying antibiotic resistance genes or mobile genetic elements causing multidrug resistance phenomena were identified, suggesting that P. vermicola in its natural environment may not be prone to acquiring such genetic elements.
Article
Biotechnology & Applied Microbiology
Yamile Baro, Christina Schuster, Yohana Gato, Maria Elena Marquez, Andreas Leclerque
Summary: Entomopathogenic fungal isolates from Cuba were characterized and two M. anisopliae isolates were identified as potential bio-control agents against the sweet potato weevil. Among them, isolate LBM-267 showed high virulence and abundant conidial production, making it a promising candidate for further research and development activities for biological control of this economically important agricultural pest.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)