Article
Microbiology
Sergi Garcia-Barreda, Pedro Marco, Gregory Bonito, Javier Parlade, Sergio Sanchez, Vicente Gonzalez, Inmaculada Larena, Gian Maria Niccolo Benucci
Summary: The study found that truffle nests did not promote the growth of black truffle mycelium, but led to a less diverse fungal community. The nests acted as an open niche for fungal colonization and helped alleviate ectomycorrhizal competition. Contrary to the hypothesis, the nests did not improve mycelial growth or negatively affect the abundance of other ectomycorrhizal fungi, suggesting that ectomycorrhizal competition does not play a role in the nest effect.
FEMS MICROBIOLOGY ECOLOGY
(2023)
Article
Plant Sciences
Yasmine Pinuela, Josu G. Alday, Daniel Oliach, Carles Castano, Francesc Bolano, Carlos Colinas, Jose Antonio Bonet
Summary: The study found that under white mulch, the mycelial development of black truffle was the greatest, with irrigation combined with white mulch favoring T. Melanosporum while the control irrigation treatment favored T. aestivum. Mulch treatments were beneficial for seedling growth and showed significant relationships between root collar diameter, root growth, and the amount of mycelial biomass in soil.
Article
Microbiology
Davide Sisti, Sabrina Donati Zeppa, Antonella Amicucci, Livia Vittori Antisari, Gilmo Vianello, Federico Puliga, Pamela Leonardi, Mirco Iotti, Alessandra Zambonelli
Summary: This study investigated the tolerance of T. borchii to high levels of Pb and the molecular mechanisms involved, revealing a high tolerance and the sequestration of Pb outside the hyphae as a crystal of Pb oxalate as the main mechanism. Furthermore, T. borchii mycorrhization significantly influenced the uptake and translocation of other elements in Q. cerris plants.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Agronomy
Yanliang Wang, Ran Wang, Bin Lu, Alexis Guerin-Laguette, Xinhua He, Fuqiang Yu
Summary: The study found that Tuber melanosporum ectomycorrhizal colonization may regulate carbon economy and rhizosphere bacterial communities of Quercus mongolica seedlings grown in a previously sterilized peat-based substrate, to promote plant growth and nutrient cycling.
Article
Forestry
Celia Herrero de Aza, Sergio Armenteros, James McDermott, Stefano Mauceri, Jaime Olaizola, Maria Hernandez-Rodriguez, Olaya Mediavilla
Summary: This study conducted a comprehensive analysis of the fungal and bacterial communities associated with T. melanosporum oak plantations. The competitive effect of T. melanosporum on other fungal species was demonstrated, and its association with specific bacteria and fungi was identified.
Article
Plant Sciences
Juan Chen, Herminia De la Varga, Flora Todesco, Pauline Beacco, Elena Martino, Francois Le Tacon, Claude Murat
Summary: The study aimed to assess the distribution of T. melanosporum mating types under productive and non-productive trees, revealing that both mating types were more commonly found under productive trees.
Article
Forestry
Ismail Sen, Yasmine Pinuela, Josu G. Alday, Daniel Oliach, Francesc Bolano, Juan Martinez de Aragon, Carlos Colinas, Jose Antonio Bonet
Summary: The study evaluated the effects of mulching on Tuber melanosporum mycelium biomass and seedling growth in a plantation, and found that mycelium biomass tended to be higher on seedlings after eight-year mulching compared to five-year mulching. A positive relationship between mycelium biomass and seedling root collar diameter was also observed.
Article
Ecology
Eva Gomez-Molina, Sergio Sanchez, Meritxell Puig-Pey, Sergi Garcia-Barreda
Summary: This study investigated the temporal dynamics of mating types in nursery seedlings of Quercus ilex and Quercus faginea, as well as the influence of cultural conditions in the nursery. The results indicated that there was a trend for one of the mating types to dominate over the other from the first to the second year in the nursery.
Article
Food Science & Technology
Win Nee Phong, Hani Al-Salami, Mark R. Gibberd, Gary A. Dykes, Alan D. Payne, Ranil Coorey
Summary: This study aimed to develop a novel technique using beta-cyclodextrin encapsulation to retain and stabilize compounds contributing to truffle aroma. The findings suggest that encapsulation of volatile compounds in beta-cyclodextrin can improve the volatile stability of truffle products and allow for longer storage times, while considering the potential microbial contamination in the encapsulation process.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Kenny S. O. Choo, Maike Bollen, Gary A. Dykes, Ranil Coorey
Summary: The study focused on the aroma profile of West Australian black Perigord truffle over a 14 day storage period, identifying significant changes in key volatiles. A comparison with the published aroma profile of European grown black Perigord truffle revealed both similarities and differences. Principle component analysis indicated that major changes in the truffle aroma profile occurred within the first 7 days of storage.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biology
Vasiliki Barou, Ana Rincon, Cinta Calvet, Amelia Camprubi, Javier Parlade
Summary: Truffle culture is profitable, but there is a waiting period for peak production. Adding secondary crops like medicinal and aromatic plants can enhance sustainability. This study explores relationships between oak and aromatic plants, as well as mycorrhizal fungi, revealing competition effects and the need to manage arbuscular mycorrhizal fungi in truffle plantations.
Article
Biotechnology & Applied Microbiology
Lei Ye, Xuezhen Yang, Bo Zhang, Jie Zhou, Hong Tian, Xiaoping Zhang, Xiaolin Li
Summary: The study describes the spatial and temporal dynamics of soil physicochemical properties and fungal communities associated with Tuber indicum-producing plots. It reveals significant seasonal variation in soil properties and fungal communities. This research highlights the complexity of ecological dynamics during the annual growth cycle of Tuber indicum and emphasizes the importance of core communities in truffle plots for the protection of native truffle ecosystems and controlling fungal contamination in artificial truffle plantations in China.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Fabiano Sillo, Marzia Vergine, Andrea Luvisi, Alice Calvo, Gianniantonio Petruzzelli, Raffaella Balestrini, Stefano Mancuso, Luigi De Bellis, Federico Vita
Summary: This study applied metabarcoding to assess the differences among samples of T. magnatum collected from different areas in Tuscany, Italy. The results showed differences in microbial composition between different parts of the truffle (gleba and peridium) as well as between productive and unproductive soils, with distinctive associated taxa. Furthermore, the findings indicated specific characteristics associated with truffle collection areas, suggesting microbial selection related to different environments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Emilia Chuina Tomazeli, Manuel Alfaro, Alessandra Zambonelli, Edurne Garde, Gumer Perez, Idoia Jimenez, Lucia Ramirez, Hesham Salman, Antonio G. Pisabarro
Summary: In this study, a new strain of Tuber borchii (strain SP1) was isolated and cultured, and its transcriptome was analyzed under different in vitro culture conditions. The results showed that the highest growth of T. borchii SP1 was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22°C. The transcription profile suggests that T. borchii uses fermentative metabolism rather than respiratory metabolism in these cultures, and there is a reduced expression of genes belonging to secondary metabolite clusters, whereas there is a significative transcription of those involved in producing volatile aromatic compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Pamela Leonardi, Riccardo Baroni, Federico Puliga, Mirco Iotti, Elena Salerni, Claudia Perini, Alessandra Zambonelli
Summary: The white truffle's mycorrhizas are difficult to find in the soil, but their mycelium can be detected by DNA extraction, leading to the discovery of co-occurrence with other Tuber species. The study suggests that the mycelial network of white truffle is more extensive than their mycorrhizal distribution, and competition between different Tuber species seems to only occur for root colonization.
Article
Plant Sciences
Yasmine Pinuela, Josu G. Alday, Daniel Oliach, Carles Castano, Francesc Bolano, Carlos Colinas, Jose Antonio Bonet
Summary: The study found that under white mulch, the mycelial development of black truffle was the greatest, with irrigation combined with white mulch favoring T. Melanosporum while the control irrigation treatment favored T. aestivum. Mulch treatments were beneficial for seedling growth and showed significant relationships between root collar diameter, root growth, and the amount of mycelial biomass in soil.
Article
Soil Science
Irene Adamo, Carles Castano, Jose Antonio Bonet, Carlos Colinas, Juan Martinez de Aragon, Josu G. Alday
Summary: Soil fungi play a critical role in forest ecosystem processes. This study examined the relative importance of soil characteristics, geographical distance, and forest structure in shaping soil fungal communities in Mediterranean pine forests. Results indicate that soil parameters have the greatest impact on fungal community variation, with mycorrhizal and saprotrophic communities showing different response patterns to soil factors and geographic distance. Forest structure, on the other hand, did not significantly influence soil fungal communities in these forests.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Tomas Cejka, Elizabeth L. Isaac, Daniel Oliach, Fernando Martinez-Pena, Simon Egli, Paul Thomas, Miroslav Trnka, Ulf Buntgen
Summary: Climate change poses a threat to truffle cultivation and its prices. The future trends in temperature, precipitation, and soil moisture will lead to a decrease in truffle production rates and an increase in prices. However, the predicted increase in summer precipitation in Australasia may alleviate water scarcity and support higher yields of more affordable truffles.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Article
Ecology
Mark A. Anthony, Thomas W. Crowther, Sietse van der Linde, Laura M. Suz, Martin Bidartondo, Filipa Cox, Marcus Schaub, Pasi Rautio, Marco Ferretti, Lars Vesterdal, Bruno De Vos, Mike Dettwiler, Nadine Eickenscheidt, Andreas Schmitz, Henning Meesenburg, Henning Andreae, Frank Jacob, Hans-Peter Dietrich, Peter Waldner, Arthur Gessler, Beat Frey, Oliver Schramm, Pim van den Bulk, Arjan Hensen, Colin Averill
Summary: This study investigates the effects of ectomycorrhizal fungi (EMF) on tree growth and finds that the composition of EMF is strongly correlated with tree growth rate. The study also suggests that the proportion of nitrogen acquisition genes and exploration types of EMF communities play a role in determining tree growth.
Article
Soil Science
Daniel Oliach, Carles Castano, Christine R. Fischer, Dominique Barry-Etienne, Jose Antonio Bonet, Carlos Colinas, Jonas Oliva
Summary: This study investigates the expansion of T. melanosporum mycelium and its interactions with other fungal communities in black truffle plantations. The findings suggest that T. melanosporum develops steadily and negatively correlates with other ectomycorrhizal fungi, arbuscular mycorrhizal fungi, and non-root associated fungi. Additionally, the mating type frequency remains constant over the years, indicating that T. melanosporum can colonize and dominate the surrounding soil in mature Quercus ilex L. plantations.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Article
Microbiology
J. Kowal, E. Arrigoni, S. Jarvis, S. Zappala, E. Forbes, M. I. Bidartondo, L. M. Suz
Summary: This study reveals significant relationships between fine root endophyte mycorrhizal fungi and soil variables such as S, P, C:N ratio, electrical conductivity, and manganese (Mn). It also highlights the negative relationship between estimated nitrogen deposition and the colonization of mycorrhizal fungi. The research identifies a greater diversity of Endogonales operational taxonomic units (OTUs) in the roots of Lycopodiella inundata and shows that OTU richness correlates negatively with soil C:N ratio while community composition is mainly influenced by soil P. These findings suggest that fine root endophyte mycorrhizal fungi have distinct ecological preferences in response to edaphic variables and air pollution.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Entomology
Emigdio Jordan Munoz-Adalia, Jaher Ahmed, Carlos Colinas
Summary: This study investigated the summer flight phenology of the oak pinhole borer in northeastern Spain, and found that population peaks occurred from late May to mid-August. The results suggest that the population dynamics of this pest are influenced by date and climatic factors.
JOURNAL OF APPLIED ENTOMOLOGY
(2022)
Article
Behavioral Sciences
Tomas Cejka, Paul W. Thomas, Daniel Oliach, Ulrich Stobbe, Simon Egli, Willy Tegel, Giada Centenaro, Ludger Sproll, Istvan Bagi, Miroslav Trnka, Ulf Buentgen
Summary: This study examines the factors that affect the hunting performance of truffle dogs, including genetic disposition, education, experience, personality, and environmental conditions. By combining published evidence with the knowledge of truffle hunters, the researchers propose five experiments to further investigate the impact of weather conditions, soil parameters, and bacterial composition on the dispersal of truffle volatiles perceived by trained dogs. They also suggest exploring the use of multiple dogs to increase the success rate of truffle hunting and emphasize the importance of interdisciplinary research.
JOURNAL OF VETERINARY BEHAVIOR-CLINICAL APPLICATIONS AND RESEARCH
(2022)
Article
Mycology
Tiara S. Cabral, Gislaine C. S. Melanda, Nathalia Mendonca de Assis, Clark Ovrebo, Iuri Goulart Baseia, Maria P. Martin
Summary: In this study, the systematic position and species diversity of the genus Staheliomyces E. Fisch. were investigated based on collections from Brazil and Costa Rica. Four new species were described using morphological and molecular phylogenetic analyses, and the genus was proposed to be emended. The phylogenetic position of the genus was established, and it clusters with Xylophallus (Schltdl.) E. Fisch. in an exclusively neotropical clade.
MYCOLOGICAL PROGRESS
(2022)
Article
Agronomy
Emigdio Jordan Munoz-Adalia, Andreu Meijer, Carlos Colinas
Summary: This study investigates the symbiotic relationship between D. corticola and P. cylindrus and develops a quick detection method for D. corticola carried by insects. The results show a higher probability of higher spore loads in May and August.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Tiara Sousa Cabral, Julieth de Oliveira Sousa, Bianca da Denise Barbosa da Silva, Maria Paz Martin, Charles Roland Clement, Iuri Goulart Baseia
Summary: Geastrum is a worldwide distributed genus with diverse species, easily recognized by the star-like basidioma. The most recent classification subdivided the genus into 14 sections. During field trips in Brazilian Amazonia, a stipitate species of Geastrum, named Geastrum squameoramulosum sp. nov., was found. It is currently known to be the only species discovered in the Amazon rainforest.
PLANT SYSTEMATICS AND EVOLUTION
(2022)
Article
Microbiology
Amaya Alvarez-Lafuente, Luis F. Benito-Matias, Mercedes Uscola, Laura M. Suz
Summary: The study found that using a substrate with high levels of active calcium can enhance the adaptation of chestnut seedlings to black truffles and promote mutualistic symbiosis between the two. Inoculated chestnut plants showed higher root dry weight, water potential values, and calcium content, indicating a positive effect of the truffle on the host plant.
Article
Ecology
Mauro Rivas-Ferreiro, Shannon M. Skarha, Franck Rakotonasolo, Laura M. Suz, Bryn T. M. Dentinger
Summary: Madagascar is known for its rich diversity and endemism of fauna and flora. However, little attention has been given to fungi in diversity and evolution studies on the island. This study collected fungal sporocarps and ectomycorrhizal roots in Madagascar and analyzed their DNA sequences to reveal the overall fungal diversity and endemic species. The study also investigated the timing of arrival of ectomycorrhizal species in Madagascar through phylogenetic analysis.
Article
Forestry
Ismail Sen, Yasmine Pinuela, Josu G. Alday, Daniel Oliach, Francesc Bolano, Juan Martinez de Aragon, Carlos Colinas, Jose Antonio Bonet
Summary: The study evaluated the effects of mulching on Tuber melanosporum mycelium biomass and seedling growth in a plantation, and found that mycelium biomass tended to be higher on seedlings after eight-year mulching compared to five-year mulching. A positive relationship between mycelium biomass and seedling root collar diameter was also observed.
