Article
Biochemical Research Methods
Oguzhan Begik, Gregor Diensthuber, Huanle Liu, Anna Delgado-Tejedor, Cassandra Kontur, Adnan Muhammad Niazi, Eivind Valen, Antonio J. Giraldez, Jean-Denis Beaudoin, John S. Mattick, Eva Maria Novoa
Summary: RNA polyadenylation plays a central role in RNA maturation, fate, and stability. Nanopore 3' end-capture sequencing (Nano3P-seq) is a method that uses nanopore cDNA sequencing to simultaneously quantify RNA abundance, tail composition, and tail length dynamics at per-read resolution. This method can accurately estimate transcript levels, tail lengths, and tail composition heterogeneity in individual reads, with minimal library preparation biases, both in the coding and non-coding transcriptome.
Article
Biochemistry & Molecular Biology
Ganesh R. Koshre, Feba Shaji, Neeraja K. Mohanan, Nimmy Mohan, Jamshaid Ali, Rakesh S. Laishram
Summary: Star-PAP is a non-canonical poly(A) polymerase that selects mRNA targets for polyadenylation. HITS-CLIP technology was used to map the cellular binding landscape of Star-PAP and its global mRNA association mechanism. Star-PAP is highly associated with both the 3'-UTR and CDS regions, with a novel role in mRNA metabolism that requires RBM10-mRNA association.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Yujiao Wu, Ying Yu, Quan Sun, Yixiang Yu, Jie Chen, Tian Li, Xianzhi Meng, Guoqing Pan, Zeyang Zhou
Summary: In this study, a gene called TRAPa (HNbTRAPa) was characterized in the microsporidia Nosema bombycis. TRAPa is a functional component of the ER translocon and is involved in substrate-specific protein translocation, a feature conserved in animals but absent in most fungi. The coding sequence of HNbTRAPa is longer than other homologs in microsporidia, and two mRNA isoforms resulting from non-canonical alternative polyadenylation were identified. The localization of HNbTRAPa in the microsporidia showed different patterns during the proliferation and spore stages. This study demonstrates the presence of post-transcriptional regulation and expands the repertoire of mRNA isoforms in microsporidia.
Article
Multidisciplinary Sciences
Yange Cui, Luyang Wang, Qingbao Ding, Jihae Shin, Joel Cassel, Qin Liu, Joseph M. Salvino, Bin Tian
Summary: JTE-607 is the first known CPA inhibitor in mammalian cells, which perturbs gene expression through transcriptional readthrough and alternative polyadenylation. Sensitive genes have similar features as PCF11 knockdown, indicating a unified transcriptomic signature of CPAi. JTE-607 inhibits APA sites in a usage-dependent manner and causes S phase crisis, making it synergistic with DNA damage repair inhibitors.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Buki Kwon, Mervin M. Fansler, Neil D. Patel, Jihye Lee, Weirui Ma, Christine Mayr
Summary: Multi-UTR genes express their alternative 3' UTR isoforms in a cell type-specific manner. Transcriptional enhancers, along with transcription factors, regulate the expression of these isoforms by influencing the cleavage activity of polyadenylation sites.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ilia G. Ustyantsev, Olga R. Borodulina, Dmitri A. Kramerov
Summary: SINEs require specific beta and tau regions for polyadenylation of their transcripts, involving multiple proteins in the process, and utilize some proteins involved in RNA polymerase II transcription to polyadenylate their own RNA.
Article
Cell Biology
Chrysoula Komini, Irini Theohari, Andromachi Lambrianidou, Lydia Nakopoulou, Theoni Trangas
Summary: Poly(A) polymerases add poly(A) tail to most eukaryotic mRNA, and are linked to proliferation and cancer. Research on breast cancer samples revealed that overexpression of PAPOLA is associated with aggressive triple-negative subtype, impacting cell proliferation and growth through modulation of CCND1 mRNA 3' UTR length.
JOURNAL OF CELL SCIENCE
(2021)
Meeting Abstract
Orthopedics
M. Bollmann, T. Gronau, T. Pap, C. H. Lohmann, J. Bertrand
OSTEOARTHRITIS AND CARTILAGE
(2017)
Meeting Abstract
Orthopedics
A. Held, C. Prein, A. Aszodi, D. Kronenberg, U. Hansen, H. Clausen-Schaumann, T. Pap, J. Bertrand
OSTEOARTHRITIS AND CARTILAGE
(2017)
Article
Biochemistry & Molecular Biology
Ganesh R. Koshre, Feba Shaji, Neeraja K. Mohanan, Nimmy Mohan, Jamshaid Ali, Rakesh S. Laishram
Summary: Star-PAP is a non-canonical poly(A) polymerase that selects mRNA targets for polyadenylation. HITS-CLIP technology was used to map the cellular binding landscape of Star-PAP and its global mRNA association mechanism. Star-PAP is highly associated with both the 3'-UTR and CDS regions, with a novel role in mRNA metabolism that requires RBM10-mRNA association.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Andrea Janicova, Nils Becker, Baolin Xu, Marija Simic, Laurens Noack, Nils Wagner, Andreas J. Muller, Jessica Bertrand, Ingo Marzi, Borna Relja
Summary: Severe traumatic injury can lead to immune response imbalance and the development of complications. Neutrophils and monocytes show phenotypic shifts post-injury, with certain subsets displaying increased functional abnormalities that may weaken antimicrobial functions.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Neeraja K. Mohanan, Feba Shaji, Ganesh R. Koshre, Rakesh S. Laishram
Summary: Alternative polyadenylation (APA) is a molecular mechanism involving multiple polyadenylation sites in pre-mRNA processing, leading to transcripts of varying lengths from a single gene. APA plays a crucial role in transcript length, coding potential, and protein function, and is considered an important regulatory mechanism in the pathophysiology of human diseases. Targeting APA regulators could have therapeutic significance in combatting drug resistance in cancer and other complex diseases.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Article
Materials Science, Multidisciplinary
Maximilian Regenberg, Janett Schmelzer, Georg Hasemann, Jessica Bertrand, Manja Krueger
Summary: This study reports on the properties and biocompatibility evaluation of a novel biomedical alloy Ta-Nb-Ti, which showed competitive osteoblast attachment, comparable fibrosis markers expressions, and significantly reduced inflammatory capacity, indicating high potential for future biomedical material applications.
Article
Cell Biology
Florian Haag, Anjana Manikkam, Daniel Kraft, Caroline Baer, Vanessa Wilke, Aleksander J. Nowak, Jessica Bertrand, Jazan Omari, Maciej Pech, Severin Gylstorff, Borna Relja
Summary: This study identified the immune profiles and cell origins of EVs in plasma of patients with cholangiocellular carcinoma, providing important insights for the development of therapeutic strategies for CCA and suggesting potential diagnostic or prognostic relevance of specific EVs.
Article
Chemistry, Physical
Maria Herbster, Karsten Harnisch, Paulina Kriegel, Andreas Heyn, Manja Krueger, Christoph H. Lohmann, Jessica Bertrand, Thorsten Halle
Summary: This study analyzed the microstructure and properties of TiAl6V4 alloy through thermal treatments and found that the acicular, martensitic microstructure exhibited higher hardness, corrosion resistance, and wear resistance. However, under severe inflammatory crevice conditions, selective corrosion occurred. Additionally, the microstructure also affected cell attachment.
Article
Biochemistry & Molecular Biology
Saliha Harrach, Jasmin Haag, Martin Steinbuechel, Rita Schroeter, Ute Neugebauer, Jessica Bertrand, Giuliano Ciarimboli
Summary: The study found that Masitinib interacts with various OCTs, particularly OCT1 and hOCT2, to effectively transport across cell membranes. There are important differences in the interaction between mouse and human OCTs. Further research is needed to understand the in vitro and in vivo properties of Masitinib and its uptake mechanisms related to transporters, in order to contribute to successful and responsible therapy employment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Nimmy Francis, Malaya R. Behera, Kathiresan Natarajan, Rakesh S. Laishram
Summary: RNA 39-end polyadenylation plays a role in stress response in Escherichia coli. The poly(A) polymerase I (PAPI) is involved in this process, and its activity is inhibited by tyrosine phosphorylation at residue Y202, which is enhanced during stress. The bacterial tyrosine kinase Wzc phosphorylates PAPI-Y202, and its mutation renders cells more sensitive to stress. This phosphorylation-dependent stress tolerance mechanism operates independently of the primary stress regulator RpoS.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Fenna Brunken, Tristan Senft, Maria Herbster, Borna Relja, Jessica Bertrand, Christoph H. Lohmann
Summary: Aseptic loosening is mainly caused by wear particles induced inflammatory response and subsequent bone loss. This study investigated the activation of the NLRP3 inflammasome by different metal particles in in vitro and in vivo conditions. The results showed that CoCrMo particles induced more inflammasome formation compared to TiAlV particles. Additionally, NRLP3 inflammasome activation was increased only by CoNiCrMo particles. These findings suggest that different alloys activate different inflammatory pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anja Feldmann, Yvonne Nitschke, Franziska Linss, Dennis Mulac, Sina Stuecker, Jessica Bertrand, Insa Buers, Klaus Langer, Frank Rutsch
Summary: This study combined controlled drug delivery by nanoparticles and active targeting by antibody conjugation to effectively dissolve aortic valve calcifications. This method holds promising clinical applications for treating aortic valve stenosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Mechanical
Maria Herbster, Rostyslav Nizinkovskyi, Miriam Bollmann, Dirk Bartel, Christoph H. Lohmann, Manja Krueger, Thorsten Halle, Jessica Bertrand
Summary: The rheological properties of synovial fluid have a significant impact on the friction and wear performance of total joint replacements. Synthetic fluid constituents, such as hyaluronic acid, were found to play a crucial role in shear-thinning behavior. Mathematical modeling allowed for reliable simulation of human synovial fluid flow, highlighting the need for modified lubricants in in vitro wear tests for accurate representation of clinical conditions.
Meeting Abstract
Orthopedics
J. Sherwood, J. Bertrand, F. Dell'Accio, T. Papy
OSTEOARTHRITIS AND CARTILAGE
(2017)
Meeting Abstract
Orthopedics
A. Boettcher, J. Stolberg-Stolberg, M. Raschke, T. Pap, J. Bertrand
OSTEOARTHRITIS AND CARTILAGE
(2017)
