Article
Multidisciplinary Sciences
Ganghua Yang, Zhengyang Lu, Fandi Meng, Yong Wan, Lei Zhang, Qinhong Xu, Zheng Wang
Summary: This study aimed to investigate the biological function and diagnostic and prognostic value of miR-141 in gallbladder cancer (GBC). The results showed that miR-141 was highly expressed in GBC cells but lowly expressed in normal tissues. Elevated plasma miR-141 expression was also detected in GBC patients and could serve as a more valuable diagnostic biomarker for GBC. Moreover, high plasma miR-141 expression was closely associated with tumor invasion, lymph node metastasis, and advanced pathological TNM stage, and it was also an independent prognostic factor for predicting poorer survival in GBC patients. These findings suggest that miR-141 may be a potential biomarker for diagnosis, prognosis, and treatment of GBC.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Aline Freville, Benedicte Gnangnon, Annie Z. Tremp, Caroline De Witte, Katia Cailliau, Alain Martoriati, El Moukthar Aliouat, Priyanka Fernandes, Cerina Chhuon, Olivier Silvie, Sabrina Marion, Ida Chiara Guerrera, Johannes T. Dessens, Christine Pierrot, Jamal Khalife
Summary: This study reveals the regulation mechanism of key enzyme PP1 in Plasmodium development, including the formation of PbLRR1 complex and its inhibition on PP1 phosphatase activity. Depletion of PbLRR1 negatively affects oocyst development and controls the phosphorylation status of multiple proteins through interaction with new partners.
Article
Biochemistry & Molecular Biology
Yuan Shao, Wanli Ren, Hao Dai, Fangli Yang, Xiang Li, Shaoqiang Zhang, Junsong Liu, Xiaobao Yao, Qian Zhao, Xin Sun, Zhiwei Zheng, Chongwen Xu
Summary: Papillary thyroid carcinoma (PTC) is a common subtype of thyroid carcinoma with a high relapse rate. This study investigated the role of S-phase kinase-associated protein 2 (SKP2) in PTC. The results showed that upregulated expression of SKP2 in PTC tissues was associated with clinical diagnosis. Knockdown of SKP2 inhibited cell growth and proliferation, induced apoptosis, and promoted cell autophagy under glucose deprivation. SKP2 interacted with PHLPP1 and activated AKT-related pathways. This study provides new insight into the molecular basis of SKP2 in PTC progression.
MOLECULES AND CELLS
(2023)
Article
Cell Biology
Soomin Son, Baekgyu Kim, Jihye Yang, V. Narry Kim
Summary: In this study, the researchers explore the role of the N-terminal proline-rich disordered domain (PRD) of DROSHA in miRNA processing. They find that the PRD specifically promotes the maturation of intronic miRNAs, independent of splicing reactions. They also discover that PRD is more important for rapidly evolving miRNAs, suggesting a role in miRNA evolution.
GENES & DEVELOPMENT
(2023)
Review
Biochemistry & Molecular Biology
HyeIn Jang, Payton Stevens, Tianyan Gao, Emilia Galperin
Summary: The review highlights the crucial role of LRR proteins as signaling scaffolds in cellular functions, focusing on the mechanisms and roles of Shoc2 and Erbin in normal development and disease. The interactions of these LRR scaffold proteins with various partners in cellular processes and their unexpected functions in human pathologies are discussed, emphasizing their potential as therapeutic targets and biomarkers.
Article
Biotechnology & Applied Microbiology
Soohyun Oh, Sejun Kim, Hyo-Jeong oPark, Myung-Shin Kim, Min-Ki Seo, Chih-Hang Wu, Hyun-Ah Lee, Hyun-Soon Kim, Sophien Kamoun, Doil Choi
Summary: In this study, multiple functional NLRs were identified from nonhost pepper that can recognize effectors of the potato late blight pathogen Phytophthora infestans and confer disease resistance in a surrogate system. These findings suggest that nonhost NLRs could be a valuable resource for developing crops with durable resistance against fast-evolving pathogens.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemical Research Methods
Kelly Snead, Vanessa Wall, Hannah Ambrose, Dominic Esposito, Matthew Drew
Summary: This study demonstrates that the co-expression of SUGT1 chaperone with polycistronic protein expression in baculovirus-infected insect cells can greatly enhance the production yield and quality of recombinant proteins.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Biochemistry & Molecular Biology
Abraham Takkouche, Xinru Qiu, Mayya Sedova, Lukasz Jaroszewski, Adam Godzik
Summary: Leucine Rich Repeat (LRR) domains, present in various proteins, play important roles in protein-protein interactions and ligand recognition. The least characterized class of LRR proteins, TpLRR-like LRRs, have unique structural features and sequence patterns. These proteins are involved in interactions between human pathogens and the host immune system. This study analyzed the relation of conserved residues, specific structural features, and functions of these proteins using experimental structures and AlphaFold models.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christina S. Kim, Anne M. Brown, Tijana Z. Grove, Felicia A. Etzkorn
Summary: Novel consensus leucine-rich repeat proteins (CLRR4-8) based on the LRR domain of NOD-like receptors show promise in high-affinity binding to bacterial cell wall component MDP. The biphasic binding curves and docking studies suggest multi-MDP binding, indicating potential for future pathogen biosensor design.
Article
Plant Sciences
Siddra Ijaz, Imran Ul Haq, Hafiza Arooj Razzaq, Bukhtawer Nasir, Hayssam M. Ali, Sukhwinder Kaur
Summary: This study identified differentially expressed genes in Dalbergia sissoo that play a role in resistance against dieback disease. These genes are involved in stress response and regulation of immune responses, contributing to the plant's ability to defend against pathogens.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Lais Rosa Viana, Gabriela de Matuoka e Chiocchetti, Lucas Oroy, Willians Fernando Vieira, Estela Natacha Brandt Busanello, Ana Carolina Marques, Carla de Moraes Salgado, Alexandre Leite Rodrigues de Oliveira, Andre Schwambach Vieira, Paula Saenz Suarez, Lizandra Maia de Sousa, Bianca Gazieri Castelucci, Anibal Eugenio Vercesi, Silvio Roberto Consonni, Maria Cristina Cintra Gomes-Marcondes
Summary: A leucine-rich diet may prevent muscle atrophy in cancer cachexia patients, improve muscle strength and behavior performance, and decrease some protein degradation markers.
Article
Biotechnology & Applied Microbiology
Jixin Jiang, Hui Ge, Jie Yang, Yunfei Qiao, Xingxiang Xu, Yanming Geng
Summary: This study found that circRNA_PTPRA significantly reduces lung cancer cell proliferation and induces apoptosis by regulating the expression of miR-582-3p.
Article
Environmental Sciences
Teng Hu, Di Li, TiePing Fan, XuSheng Zhao, ZhongJun Chen
Summary: Numerous studies have found that circular RNA (circRNA) plays an important role in cerebral ischemia-reperfusion (CIR) injury. A recent study discovered a novel circRNA, circCRIM1, which was highly expressed in a CIR model. This study aimed to investigate the role of circCRIM1 in CIR-induced neuronal apoptosis. The results showed that circCRIM1 was up-regulated, while miR-141-3p was down-regulated in the CIR model. Knocking down circCRIM1 reduced apoptosis and oxidative stress, while overexpression of circCRIM1 accelerated apoptosis and disrupted redox balance.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Infectious Diseases
Bo-Yang Tsai, Pei-Jane Tsai, Ching-Chi Lee, Chun-Wei Chiu, Yi-Hsin Lai, Jen-Chieh Lee, Wen-Chien Ko, Yuan-Pin Hung
Summary: This study analyzed the influence of NLRP1 polymorphisms on C. difficile colonization (CdC) or infection (CDI). The CC genotype in NLRP1 rs3744717 was associated with an increased risk of CdC, while the CC+GC genotype in NLRP1 rs878329 was associated with a higher risk of CDI.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Cell Biology
Patrick Ehm, Nina Nelson, Susanne Giehler, Matthias Schaks, Bettina Bettin, Johannes Kirchmair, Manfred Juecker
Summary: This study investigated patient-derived mutations in the phosphatase domain of SHIP1 and found that two mutations had reduced protein expression and enzymatic activity, while two other mutations had reduced activity but comparable expression levels. Overall, the mutations significantly affected the PI3K/AKT/mTOR pathway, either by decreasing SHIP1 activity or expression.
CELLULAR SIGNALLING
(2023)