Article
Biochemistry & Molecular Biology
Kazutaka Murayama, Miyuki Kato-Murayama, Tomohiro Sato, Toshiaki Hosaka, Kanako Ishiguro, Takayuki Mizuno, Kazunori Kitao, Teruki Honma, Shigeyuki Yokoyama, Yoshikazu Tanaka, Mikako Shirouzu
Summary: The acylation of anthocyanins contributes to structural diversity, with aromatic acylation being responsible for the blue color of anthocyanins and certain flowers. Gt5,3'AT catalyzes the acylation of glucosyl moieties at specific positions, exhibiting unique enzymatic activity. Structural analysis of Gt5,3'AT revealed insights into the underlying mechanism of acylation at double positions.
Article
Biochemistry & Molecular Biology
Tomoyuki Takase, Motoki Shimizu, Shigekazu Takahashi, Keiichirou Nemoto, Fumina Goto, Chiharu Yoshida, Akira Abe, Masahiro Nishihara
Summary: This study investigated the molecular mechanisms underlying the control of flowering time in Japanese cultivated gentians. Transcriptome profiling and analysis of transcription factor genes revealed their coexpression with the flowering time-related gene GtFT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hyeon-Min Kim, Jun-Hyeok Kim, Da-Hyun Lee, Young-Ho Jung, Chung-Youl Park, Mi-Hyun Lee, Kyeong-Min Kim, Jae-Hyeon Lee, Chae-Sun Na
Summary: The study found that the seeds of Gentiana triflora var. japonica exhibited non-deep simple morphophysiological dormancy, and the germination rate could be significantly increased through specific treatments.
Article
Plant Sciences
Rong Zhao, Shuyue Yin, Jianing Xue, Chang Liu, Yanping Xing, Haibo Yin, Xue Ren, Jixiang Chen, Dingding Jia
Summary: Three species of Gentiana were the main source for traditional Chinese medicine Longdan. A new taxonomic classification for Gentiana was proposed based on the chloroplast genomes and phylogenetic analyses, providing a theoretical foundation for molecular evolutionary research of Gentiana.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Chemistry, Applied
Dawei Chen, Shuai Fan, Zhaoyong Yang, Jungui Dai
Summary: This study reports the identification of a new plant enzyme MaCGT and its role in C-glucosylation reactions. The enzyme exhibits efficient selectivity towards structurally diverse coumarins. Additionally, the research reveals the diversity of CGTs in the plant kingdom.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Agriculture, Multidisciplinary
Yoojin Chong, Bong-Gyu Kim, Yeo-Jin Park, Youri Yang, Shin-Won Lee, Youngshim Lee, Joong-Hoon Ahn
Summary: In this study, a pathway was introduced to Escherichia coli to synthesize four flavonoid C-glucosides. The synthesis pathway involved the use of two regioselective flavonoid C-glycosyltransferases and resulted in the synthesis of isovitexin, vitexin, kaempferol 6-C-glucoside, and kaempferol 8-C-glucoside. The synthesized flavonoid C-glucosides provide a basis for investigating their novel biological properties.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Cheng-Yong Feng, Shan-Shan Li, Goro Taguchi, Qian Wu, Dan-Dan Yin, Zhao-Yu Gu, Jie Wu, Wen-Zhong Xu, Cheng Liu, Liang-Sheng Wang
Summary: This study identified two C-glycosyltransferase genes in sacred lotus and showed that they play a crucial role in the biosynthesis of flavonoids. The enzymes UGT708N1 and UGT708N2 were found to catalyze the C-glycosylation of 2-hydroxyflavanones and 2-hydroxynaringenin, forming mono-C-glycosides and di-C-glycosides. The research also proposed a new biosynthetic pathway for flavonoid di-C-glycosides and identified a novel uridine diphosphate-glycosyltransferase (UGT708N2) in N. nucifera.
Article
Biochemistry & Molecular Biology
Yuyan Yang, Zhilin He, Qihao Bing, Xinyuan Duan, Suoying Chen, Ming Zeng, Xiaogang Liu
Summary: In this study, 28 Dof genes were characterized from the 'Hongkong' kumquat cultivar genome. Four of these Dof genes were found to potentially be involved in flavonoid biosynthesis by binding to the promoter of the flavonoid C-glycosyltransferase gene. Further analysis revealed that two of these Dof genes, FcDof4 and FcDof16, activate the expression of the flavonoid C-glycosyltransferase gene by binding to its promoter. Overexpression of FcDof4 and FcDof16 increased flavonoid synthesis in kumquat fruit.
Article
Plant Sciences
Xinke Zhang, Yujing Miao, Xiao Sun, Yuan Jiang, Tiexin Zeng, Yan Zheng, LinFang Huan
Summary: In this study, we reported the complete chloroplast sequences of Gentiana triflora and conducted comparative genome analysis and phylogenetic analysis. The results enriched the genomic resources of the Gentiana genus and provided a basis for evolution and phylogeny analyses.
JOURNAL OF APPLIED BOTANY AND FOOD QUALITY
(2022)
Article
Biotechnology & Applied Microbiology
Nasanjargal Dorjjugder, Mayu Hatano, Goro Taguchi
Summary: The study successfully produced flavone and flavonol 6-C-glucosides using the Ec-WjGT1 system, with high conversion rates achieved for apigenin, kaempferol, and quercetin. Additionally, sequential feeding of substrates helped to avoid precipitation and improved efficiency of the bioconversion process.
BIOTECHNOLOGY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Junpei Abe, Yoichi Takeda, Takashi Kikuma, Yasuhiko Kizuka, Hiroyuki Kajiura, Yasuhiro Kajihara, Yukishige Ito
Summary: UDP-Glc：glycoprotein glucosyltransferase (UGGT) plays a central role in maintaining the quality control of correctly folded N-glycoproteins in the endoplasmic reticulum (ER). However, a selective and potent inhibitor against UGGT has not been identified. In this study, we report squaryl group-modified UDP analogs as potential UGGT inhibitors, with one compound showing the highest potency and suggesting a candidate for further optimization.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Florian Stieglitz, Ralf Gerhard, Rabea Hoenig, Klaudia Giehl, Andreas Pich
Summary: A Clostridioides difficile infection (CDI) is a common hospital-acquired infection worldwide. The virulence factors TcdA and TcdB inhibit small Rho-GTPases, leading to cytopathic effects, colon epithelium barrier dysfunction, and inflammation. A large-scale phosphoproteomic study revealed the central role of RAS in the glucosyltransferase-independent effect of TcdB, and identified apolipoprotein C-III (APOC3) as a potential crucial factor in CDI-induced inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Huiting Xi, Yaru Zhu, Wenwen Sun, Nan Tang, Zhengqin Xu, Xiaohong Shang, Yansheng Zhang, Huabing Yan, Changfu Li
Summary: Pueraria lobata, a traditional Chinese herb, produces puerarin, an isoflavone C-glucoside with medicinal properties. This study found that puerarin biosynthesis occurs in the roots and stems of P. lobata. RNA-sequencing analysis identified several genes involved in puerarin biosynthesis.
Article
Biology
Hou-Fu Guo, Neus Bota-Rabassedas, Masahiko Terajima, B. Leticia Rodriguez, Don L. Gibbons, Yulong Chen, Priyam Banerjee, Chi-Lin Tsai, Xiaochao Tan, Xin Liu, Jiang Yu, Michal Tokmina-Roszyk, Roma Stawikowska, Gregg B. Fields, Mitchell D. Miller, Xiaoyan Wang, Juhoon Lee, Kevin N. Dalby, Chad J. Creighton, George N. Phillips, John A. Tainer, Mitsuo Yamauchi, Jonathan M. Kurie
Summary: Guo et al. have determined the molecular basis of collagen lysyl hydroxylase 2 (LH2) substrate specificity, showing that LH2 functions not only as a telopeptidyl lysyl hydroxylase, but also as a collagen glucosyltransferase to drive lung cancer progression. The research suggests that LH2 plays a crucial role in promoting LUAD progression through a variety of mechanisms, including tLH-dependent and tLH-independent pathways.
COMMUNICATIONS BIOLOGY
(2021)
Review
Plant Sciences
Min Jiang, Ben-Wen Cui, Yan-Ling Wu, Ji-Xing Nan, Li-Hua Lian
Summary: The Gentiana genus has a long tradition of medicinal uses in Europe and Asia, with main bioactive components including iridoids, xanthones, and flavonoids showing various protective activities. Plants from this genus have potential therapeutic targets in treating certain diseases, with natural products showing no significant toxicity.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Agronomy
Masahiro Nishihara, Keisuke Tasaki, Nobuhiro Sasaki, Hideyuki Takahashi
Article
Plant Sciences
Ashita Hamachi, Masahiro Nisihara, Shiori Saito, Hojun Rim, Hideyuki Takahashi, Monirul Islam, Takuya Uemura, Toshiyuki Ohnishi, Rika Ozawa, Massimo E. Maffei, Gen-ichiro Arimura
Article
Multidisciplinary Sciences
Keisuke Tasaki, Atsumi Higuchi, Aiko Watanabe, Nobuhiro Sasaki, Masahiro Nishihara
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Keisuke Tasaki, Momo Yoshida, Minori Nakajima, Atsumi Higuchi, Aiko Watanabe, Masahiro Nishihara
Article
Multidisciplinary Sciences
Shigekazu Takahashi, Suguru Ozawa, Kintake Sonoike, Katsutomo Sasaki, Masahiro Nishihara
Article
Biotechnology & Applied Microbiology
Eri Tomizawa, Shogo Ohtomo, Kanako Asai, Yuka Ohta, Yukako Takiue, Akihiro Hasumi, Masahiro Nishihara, Takashi Nakatsuka
Summary: This study successfully induced the accumulation of betalains in ornamental lisianthus through genetic engineering, resulting in novel flower varieties with unique colors. Plants with different expression levels of genes exhibited variations in flower color, with individuals showing high expression levels producing a novel wine-red color.
PLANT BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shigekazu Takahashi, Chiharu Yoshida, Hideyuki Takahashi, Masahiro Nishihara
Summary: In this study, an EPH1-LIKE gene was identified from cultivated Japanese gentians and found to play an important role in flower senescence. Knocking out this gene using the CRISPR/Cas9 system resulted in prolonged flower longevity, demonstrating the regulatory function of EPH1L in flower senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Keiichirou Nemoto, Aiko Watanabe, Chiharu Yoshida, Masahiro Nishihara
Summary: The germination rate of T. vernicifluum seeds is low due to deep seed dormancy controlled by physical and physiological mechanisms, as well as low embryo viability. This study proposes an effective method to improve the germination rate by selecting seeds with good germination capacity based on specific gravity and inactivating their physiological dormancy through cold pretreatment.
Article
Biochemistry & Molecular Biology
Tomoyuki Takase, Motoki Shimizu, Shigekazu Takahashi, Keiichirou Nemoto, Fumina Goto, Chiharu Yoshida, Akira Abe, Masahiro Nishihara
Summary: This study investigated the molecular mechanisms underlying the control of flowering time in Japanese cultivated gentians. Transcriptome profiling and analysis of transcription factor genes revealed their coexpression with the flowering time-related gene GtFT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Go Atsumi, Satoshi Naramoto, Masahiro Nishihara, Takashi Nakatsuka, Reiko Tomita, Yosuke Matsushita, Nobue Hoshi, Asuka Shirakawa, Kappei Kobayashi, Hiroo Fukuda, Ken-Taro Sekine
Summary: This study discovered a novel virus-induced factor, KOBU, which induces gall formation and the abnormal development of lignified cells and vascular tissues in Nicotiana benthamiana. Additionally, KOBU also promotes the formation of leaf-like tissue on the abaxial side of veins in normal tobacco and gentian leaves. The molecular mechanism of KOBU provides new insights into plant-virus interactions and the regulation of vascular and leaf development.
JOURNAL OF VIROLOGY
(2023)
Article
Plant Sciences
Masahiro Nishihara, Akiko Hirabuchi, Fumina Goto, Yuzo Nishizaki, Shota Uesugi, Aiko Watanabe, Keisuke Tasaki, Rie Washiashi, Nobuhiro Sasaki
Summary: Genetic engineering was used to produce yellow-flowered gentians by introducing betaxanthin pigments. The white-flowered gentians were first created by knocking out the DFR gene, and then the betaxanthin biosynthetic genes were transformed. The resultant gentian plants exhibited vivid yellow flowers with specific expression and accumulation of seven known betaxanthins.
Article
Biochemistry & Molecular Biology
Keiichirou Nemoto, Tomoya Niinae, Fumina Goto, Naoyuki Sugiyama, Aiko Watanabe, Motoki Shimizu, Katsuhiro Shiratake, Masahiro Nishihara
Summary: This study reveals that GsPIP2;2 and GsPIP2;7, regulated by GsCPK16-dependent phosphorylation and activation, play a crucial role in the repeated flower opening of Gentian plant. Phosphorylation and Ca2+ signals are important mechanisms triggering flower re-opening.
Article
Plant Sciences
Hideyuki Takahashi, Masahiro Nishihara, Chiharu Yoshida, Kimiko Itoh
Summary: Perennial plants have a dormant period in addition to growth and flowering phases. The FT orthologs GtFT1 and GtFT2 in gentian were found to be involved in budbreak and flowering, and dormancy regulation, respectively. The two orthologs activated different transcription factors to regulate different phase transitions.
Article
Biotechnology & Applied Microbiology
Takao Koeduka, Sachiho Takarada, Koya Fujii, Akifumi Sugiyama, Kazufumi Yazaki, Masahiro Nishihara, Kenji Matsui
Summary: This study demonstrates the stable and efficient production of raspberry ketone and its glycosides in transgenic tobacco through heterologous expression, along with improved product titers by activating the phenylpropanoid pathway using a transcriptional factor from Arabidopsis thaliana. The research also shows successful metabolic flux redirection by RNA interference-mediated gene silencing to increase pathway-intermediate p-coumaroyl-CoA, resulting in transgenic lines with high raspberry ketone production.
METABOLIC ENGINEERING COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hideyuki Takahashi, Chiharu Yoshida, Takumi Takeda
JOURNAL OF APPLIED GLYCOSCIENCE
(2019)
