期刊
FEBS LETTERS
卷 589, 期 1, 页码 159-164出版社
WILEY
DOI: 10.1016/j.febslet.2014.11.041
关键词
Human cystatin C; Hereditary Cystatin C Amyloid Angiopathy; Alzheimer's disease; 3D-domain swapping; 'Aggregation-prone' pentapeptide LQVVR; Amyloid fibrils
资金
- University of Athens
Human cystatin C (HCC) is a low molecular weight member of the cystatin family (type2). HCC consists of 120 amino acids. Normally it is an inhibitor of cysteine proteases, but in pathological conditions it forms amyloid fibrils in brain arteries of young adults. An 'aggregation-prone' pentapeptide ((47)LQVVR(51)) was located within the HCC sequence using AmylPred, an 'aggregation-prone' peptide prediction algorithm developed in our lab. This peptide was synthesized and self-assembled into amyloid-like fibrils in vitro, as electron microscopy, X-ray fiber diffraction, Attenuated Total Reflectance Fourier-Transform Spectroscopy and Congo red staining studies reveal. Thus, the (47)LQVVR(51) peptide seems to have an important role in HCC fibrillization. (C) 2014 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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