期刊
FEBS LETTERS
卷 588, 期 17, 页码 2993-2999出版社
WILEY
DOI: 10.1016/j.febslet.2014.06.008
关键词
Methylotrophy; Alcohol dehydrogenase; Bacillus methanolicus
资金
- European Science Foundation (ESF) - SNF [09-EuroSYNBIO-FP-023, 31SY30-131039]
- EU-FP7 PROMYSE Products from Methanol by Synthetic Cell Factories
- Swiss National Science Foundation (SNF) [31SY30-131039] Funding Source: Swiss National Science Foundation (SNF)
In the Gram-positive methylotroph Bacillus methanolicus, methanol oxidation is catalyzed by an NAD-dependent methanol dehydrogenase (Mdh) that belongs to the type III alcohol dehydrogenase (Adh) family. It was previously shown that the in vitro activity of B. methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase. Here we show that this feature is not unique, but more widespread among type III Adhs in combination with Act or other Act-like Nudix hydrolases. In addition, we studied the effect of site directed mutations in the predicted active site of Mdh and two other type III Adhs with regard to activity and activation by Act. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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