期刊
FEBS LETTERS
卷 587, 期 23, 页码 3837-3842出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.10.011
关键词
Fatty acid elongation; Condensing enzyme; ELO
资金
- IUPUI School of Science
- Department of Chemistry and Chemical Biology
The condensation step of fatty acid elongation is the addition of a C-2 unit from malonyl-CoA to an acyl primer catalyzed by one of two families of enzymes, the 3-ketoacyl-CoA synthases and the ELO-like condensing enzymes. 3-Ketoacyl-CoA synthases use a Claisen-like reaction mechanism while the mechanism of the ELO-catalyzed condensation reaction is unknown. We have used site-directed mutagenesis of Dictyostelium discoideum EloA to identify residues important to catalytic activity and/or structure. Mutation of highly conserved polar residues to alanine resulted in an inactive enzyme strongly suggesting that these residues play a role in the condensation reaction. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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