期刊
FEBS LETTERS
卷 588, 期 3, 页码 443-449出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.11.038
关键词
Membrane microdomain; Lipid order; Fluidity; Fluorescence anisotropy; Time-resolved fluorescence
资金
- Czech Science Foundation [GACR P205/12/0720]
We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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