期刊
FEBS LETTERS
卷 587, 期 7, 页码 860-866出版社
WILEY
DOI: 10.1016/j.febslet.2013.02.037
关键词
Carbohydrate-binding agent; High-mannose N-glycan; Monomeric gp120; Surface plasmon resonance; Trimeric gp140
资金
- KU Leuven [PF 10/18, GOA 10/14]
- European Commission
- FWO-Vlaanderen [G-0528-12N]
- Netherlands Organization for Scientific Research (NWO)
- European Research Council [ERC-StG-2011-280829-SHEV]
The native HIV-1 Env complex consists of a gp120/gp41 trimer, but surface plasmon resonance (SPR)-directed binding studies for gp120-binding agents were almost exclusively performed on monomeric gp120. SPR-directed binding kinetics of monomeric gp120 and trimeric gp140 were investigated for a broad variety of envelope (Env)-binding agents. Similar kinetics for carbohydrate-binding agents (CBAs), the antibody 2G12 and sCD4 were observed, irrespective of the oligomeric state of gp120 that either contain the native mixture of complex and high-mannose N-glycans or that contain exclusively oligomannose N-glycans. The generally comparable kinetic properties of CBA, 2G12 and sCD4 binding to monomeric gp120 and trimeric gp140 indicate that monomeric gp120 is a good surrogate molecule for native HIV-1 Env trimer to investigate the binding affinities of Env-binding compounds. Structured summary of protein interactions: gp120 binds to GRFT by surface plasmon resonance (View Interaction: 1, 2) gp120 binds to UDA by surface plasmon resonance (View Interaction: 1, 2) gp120 binds to AH by surface plasmon resonance (View Interaction: 1, 2) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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