Review
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Akira Watanabe, Takahiro Shintani, Katsuya Gomi, Katsuro Yaoi
Summary: Aspergillus species are closely associated with humanity through fermentation, infectious disease, and mycotoxin contamination of food. This review focuses on the machinery of the xyloglucan degradation in Aspergillus species using glycoside hydrolases, emphasizing the crucial role of cooperative actions of these enzymes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Masahiro Watanabe, Yusuke Nakamichi, Akihiko Kameyama, Naoshi Kojima, Katsuro Yaoi
Summary: In this study, an extracellular alpha-xylosidase, AxyB, was identified and characterized in Aspergillus oryzae, belonging to glycoside hydrolase family 31 and involved in releasing D-xylose from xyloglucan oligosaccharides. The research shows that AxyB plays a key role in the degradation of xyloglucans by releasing the xylopyranosyl side chain from these oligosaccharides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Haipeng Su, Jianan Sun, Zhenrong Jia, Hongjun Zhao, Xiangzhao Mao
Summary: This review provides an overview of the promiscuous activities and substrate specificity of chitosanases, contributing important implications for the future discovery and research of promiscuous chitosanases and applications related to biomass conversion.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Dianyi Li, Zheng Wang, Yong Yu, Huirong Li, Wei Luo, Bo Chen, Guoqing Niu, Haitao Ding
Summary: A novel GH2 beta-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli and showed stable soluble form. The purified enzyme had a specific activity of 96.827 U mg(-1) at 30 degrees C using ONPG as a substrate. It had an optimum pH of 7.0 and an optimum temperature of 50 degrees C. The enzyme's activity was enhanced by some divalent cations and inhibited by EDTA. Despite being derived from a cold-adapted strain, the enzyme exhibited considerable stability against various physical and chemical elements. It also showed activity towards rare substrates. The enzyme is a promising candidate for the industrial application of beta-galactosidase.
Article
Biotechnology & Applied Microbiology
Wilaiwan Kaenying, Khuanjarat Choengpanya, Takayoshi Tagami, Pakorn Wattana-Amorn, Weeranuch Lang, Masayuki Okuyama, Yaw-Kuen Li, Atsuo Kimura, Prachumporn T. Kongsaeree
Summary: This study investigates the three-dimensional structure and catalytic properties of AnBX, a GH3 beta-xylosidase from Aspergillus niger. The study identifies the catalytic residues Asp288 and Glu500, as well as the important residues Trp86 and Cys289. The findings provide valuable insights into the enzymatic properties of AnBX for lignocellulosic biomass hydrolysis.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Mert Sagiroglugil, Fatih Yasar
Summary: The catalytic mechanism of a Ca+2-dependent family 92 alpha-mannosidase and its relationship with alpha-mannosidosis have been revealed using computational methods.
Article
Biochemistry & Molecular Biology
Emma De Beul, Alana Jongbloet, Jorick Franceus, Tom Desmet
Summary: The study focuses on the sequence diversity of Glycoside Hydrolase Family 65 (GH65) and identified specificity-determining positions through in-silico analysis of correlated mutations. By searching for amino acid motifs that differ from previously characterized enzymes, several new clades with potential new functions were identified. Three new enzymes were expressed in E. coli, with one enzyme showing high specificity in hydrolyzing specific substrates.
Article
Biotechnology & Applied Microbiology
Tomohiko Matsuzawa, Masahiro Watanabe, Yusuke Nakamichi, Hironaga Akita, Katsuro Yaoi
Summary: This paper reports the crystal structures of Metagenomic MeBglD2 complexed with various saccharides, revealing its substrate recognition mechanism. The subsite -1 of MeBglD2 is structurally similar to other GH1 enzymes, but the binding modes of the positive subsites vary depending on the type of sugar. Three residues (Glu183, Asn227, and Asn229) located at the positive subsites of MeBglD2 are involved in substrate specificity in the presence of additive sugars. Docking simulation suggests that Asn229 and Trp329 play key roles in recognizing the +1 D-glucose in cellobiose.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomoya Ota, Wataru Saburi, Linda Elizabeth Jewell, Tom Hsiang, Ryozo Imai, Haruhide Mori
Summary: In this study, a GH3 beta-glucosidase (MnBG3A) from Microdochium nivale was identified and characterized. MnBG3A showed activity on d-glucoside and slight activity on d-xyloside. Compared to beta-glucosidases from Aspergillus spp., MnBG3A has similar features but higher sensitivity to inhibitory effects.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yi-Min She, Kody Klupt, Grayson Hatfield, Zongchao Jia, Roger Y. Tam
Summary: Mannosidases are crucial enzymes that play diverse roles in cellular processes. We discovered unexpected β1-4-galactosidase activity in the α1-6-mannosidase, providing insights into its dual glycosidase specificity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Mauro Mendonca, Mario Barroca, Tony Collins
Summary: This article summarizes 9 glycoside hydrolase families containing enzymes with endo-1,4-8-xylanase activity and discusses their properties, similarities, differences, and biotechnological perspectives. It provides detailed information on substrate specificities, hydrolysis patterns, and the structural determinants of these enzymes, as well as taxonomic aspects of the source organisms. The article highlights shortcomings in current knowledge and identifies research areas that require further clarification. It aims to motivate further research on these enzymes and particularly on the lesser-known endo-1,4-8-xylanase containing families, which will serve as a foundation for the knowledge-based development of process-fitted enzymes in the biobased industries of the future.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a β-1,3-glucanase named BsGlc157A was identified from Bacteroides sp. M27. It exhibited optimal catalytic activity at pH 6.0 and 40 degrees C. BsGlc157A adopted a classic (beta/alpha)(8) TIM-barrel structure and two catalytic residues were confirmed. Moreover, BsGlc157A showed inhibitory effects on the hyphal growth of fruit pathogenic fungi, highlighting its potential as a biocontrol agent.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Zhen Qin, Sainan Yu, Kemin Zhang, Xiasen Wei, Junjie Li, Zheyi Zhang, Sibao Wan, Haiyan Gao
Summary: In this study, a GH family 157 endo-beta-1,3-glucanase (BsGlc157A) was identified and characterized from Bacteroides sp. M27. It was found to have applications in fields such as food processing, plant protection, and breweries. The study also revealed its catalytic properties and antifungal activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Anke Chen, Dan Wang, Rui Ji, Jixi Li, Shaohua Gu, Rong Tang, Chaoneng Ji
Summary: In this study, a thermostable beta-glucosidase (TsBGL) was isolated and characterized from the Thermoprotei Thermofilum sp. ex4484_79, exhibiting high activity and specific activity at elevated temperatures and pH levels. The crystal structure of TsBGL reveals a classical (alpha/beta)(8)-barrel catalytic domain, with catalytic sites including Glu210 and Glu414. TsBGL shows promise for potential industrial applications with its high thermostability and catalytic activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kanyisa Ndata, Walter Nevondo, Bongi Cekuse, Leonardo Joaquim van Zyl, Marla Trindade
Summary: In this study, a novel beta-xylosidase XylP81 from the poorly studied GH39 family was discovered, which exhibits overall kinetic properties similar to other bacterial GH39 beta-xylosidases but shows unusually high tolerance to products, making it a promising candidate for industrial applications.
Article
Biochemistry & Molecular Biology
Eva Schmitz, Savvina Leontakianakou, Patrick Adlercreutz, Eva Nordberg Karlsson, Javier A. Linares-Pasten
Summary: In this study, we discovered that the enzyme CtXyn5A from Acetivibrio thermocellus exhibits both arabinoxylanase and feruloyl esterase activities. Mutation analysis confirmed that the removal of catalytic nucleophile Glu279 abolishes all activity, and the E279S mutation increases esterase activity while removing xylanase activity. This suggests that both reactions are catalyzed in the same active site in two sequential steps. Furthermore, the esterase activity of CtXyn5A on complex carbohydrates is higher than that of a designated ferulic acid esterase, indicating that this enzyme has a dual function rather than a side activity.
Article
Food Science & Technology
Roya R. R. Sardari, Andrius Jasilionis, Nikos Tsardakas Renhuldt, Patrick Adlercreutz, Eva Nordberg Karlsson
Summary: Current derivatization-dependent approaches for amino acid composition analysis of cereal proteins have significant variability, but a novel direct, derivatization-free approach using HPAEC-PAD was successfully introduced for analysis. The approach ensured reliable detection of amino acids and oxidation products. Comparison of the original mass fraction of amino acids with the mass fraction obtained after hydrolysis showed small differences, except for a few specific amino acids. The amino acid composition estimation from sequence data indirectly confirmed the presence of unknown flour components.
JOURNAL OF CEREAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Andrius Jasilionis, Magdalena Plotka, Lei Wang, Sebastian Dorawa, Joanna Lange, Hildegard Watzlawick, Tom van den Bergh, Bas Vroling, Josef Altenbuchner, Anna-Karina Kaczorowska, Ehmke Pohl, Tadeusz Kaczorowski, Eva Nordberg Karlsson, Stefanie Freitag-Pohl
Summary: Bacteriophages encode lytic enzymes that disrupt cell walls and have potential as novel antibacterials. This study presents the functional and structural characterization of Thermus parvatiensis prophage peptidoglycan lytic amidase AmiP, an enzyme adapted to high temperatures. AmiP is shown to be highly efficient and has broad substrate specificity. It is the most thermoactive and ultrathermostable Amidase_3 type lytic enzyme characterized so far.
Article
Environmental Sciences
Yumi Kobayashi, Mohammad EL-Wali, Horour Guomundsson, Elisabet Eik Guomundsdottir, Olafur H. Friojonsson, Eva Nordberg Karlsson, Marja Roitto, Hanna L. Tuomisto
Summary: The production of fish meal and plant-based feed proteins is increasing to meet seafood demand, causing environmental impacts. Microbial proteins like single-cell proteins (SCPs) are introduced as feed alternatives to reduce negative environmental impacts. This study evaluates the environmental impacts of yeast-based SCP using oat side-stream as feedstock and highlights the importance of energy sources in determining its environmental performance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Bashar Shuoker, Michael J. Pichler, Chunsheng Jin, Hiroka Sakanaka, Haiyang Wu, Ana Martinez Gascuena, Jining Liu, Tine Sofie Nielsen, Jan Holgersson, Eva Nordberg Karlsson, Nathalie Juge, Sebastian Meier, Jens Preben Morth, Niclas G. Karlsson, Maher Abou Hachem
Summary: This study provides insights into the mechanism of mucin O-glycan degradation by Akkermansia muciniphila and the nutrient sharing between this symbiotic bacteria and the mucus-associated microbiota. They discovered new enzymes involved in the breakdown of protective caps on mucin, which promote the growth of A. muciniphila and the production of butyrate through cooperation with other bacteria.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Roland S. Kun, Sonia Salazar-Cerezo, Mao Peng, Yu Zhang, Emily Savage, Anna Lipzen, Vivian Ng, Igor V. Grigoriev, Ronald P. de Vries, Sandra Garrigues
Summary: This study deepened the understanding of the storage polysaccharide regulators AmyR and InuR in Aspergillus niger by assessing the phenotypes and transcriptomic data of different strains under various culture conditions. The results showed that AmyR has a minor contribution to sucrose and inulin utilization when InuR is active, and deletion of amyR in the Delta inuR background strain resulted in pronounced growth reduction on both substrates, particularly in solid cultures. Overall, the study highlights the importance of culture conditions in accurately studying the role of transcription factors in filamentous fungi.
Article
Biotechnology & Applied Microbiology
Leila Allahgholi, Madeleine Jonsson, Monica Daugbjerg Christensen, Andrius Jasilionis, Mehrnaz Nouri, Shahram Lavasani, Javier A. Linares-Pasten, Guomundur Oli Hreggvidsson, Eva Nordberg Karlsson
Summary: This study investigates the fermentation ability of a lactic acid bacteria consortium consisting of three Lactiplantibacillus plantarum strains and a minor amount of a Levilactobacillus brevis strain on carbohydrates available in brown seaweed. The consortium is able to ferment glucose, mannitol, galactose, mannose, and xylose, with glucose and mannitol being the preferred substrates. It utilizes different pathways for carbohydrate utilization, producing lactic acid as the main metabolite.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Dujuan Liu, Sandra Garrigues, Ronald P. de Vries
Summary: The article summarizes the benefits and challenges of using filamentous fungi for the production of heterologous proteins, as well as various techniques commonly employed to improve the production yield. These techniques include the use of strong and inducible promoters, codon optimization, more efficient signal peptides for secretion, carrier proteins, engineering of glycosylation sites, regulation of the unfolded protein response and endoplasmic reticulum associated protein degradation, optimization of the intracellular transport process, regulation of unconventional protein secretion, and construction of protease-deficient strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Sandra Garrigues, Mao Peng, Roland S. S. Kun, Ronald P. de Vries
Summary: CRISPR/Cas9 genome editing technology has been widely used in various organisms, but off-target mutations may occur due to guide RNA binding to similar nucleotides. Despite this, evaluation of mutations is rarely performed in genome editing studies.
Article
Biotechnology & Applied Microbiology
Madeleine Jonsson, Annabell Merkel, Cecilia Fredriksson, Eva Nordberg Karlsson, Karin Wendin
Summary: This study characterizes the sensory perceptions, nutritional content, and physicochemical properties of four common northern European seaweed species. The seaweed species are rich in minerals and carbohydrates, but have lower levels of protein and lipids. They are generally perceived to have high saltiness, medium umami, low bitterness and sourness, and no sweetness. Palmaria palmata stands out with its red magenta colour, sea and shellfish odour, umami taste, and firm and chewy texture.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Nutrition & Dietetics
Fang Huang, Nittaya Marungruang, Isak Martinsson, Lluis Camprubi Ferrer, Thao Duy Nguyen, Thamani Freedom Gondo, Eva Nordberg Karlsson, Tomas Deierborg, Rickard Oste, Lovisa Heyman-Linden
Summary: This study suggests that consuming a mixture of Nordic berries can improve cognitive function, counteract weight gain from high-fat diets, and have an impact on neuroinflammation. Additionally, the diet with berries resulted in significant changes in gut microbiota composition.
FRONTIERS IN NUTRITION
(2023)
Article
Food Science & Technology
Cecilia Fredriksson, Madeleine Jonsson, Annabell Merkel, Eva Nordberg Karlsson, Karin Wendin
Summary: The paper focuses on interdisciplinary research on the consumption of seaweed foods. Through a mixed methods approach, the authors explore the consumer's approach to new marine products, combining social science, food science, and a chemical and biotechnological perspective. They argue for flexibility and openness to disciplinary processes, while sharing empirical experiences in a pilot study. The goal is to deepen the understanding and connection between different disciplines in the research processes of seaweed consumption.
INTERNATIONAL JOURNAL OF GASTRONOMY AND FOOD SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Antoine Moenaert, Bryndis Bjornsdottir, Einar Baldvin Haraldsson, Leila Allahgholi, Anna Zieri, Isabella Zangl, Sigriour Siguroardottir, Johann Orlygsson, Eva Nordberg Karlsson, olafur H. Friojonsson, Guomundur oli Hreggviosson
Summary: This study successfully engineered the fermentative bacterium Thermoanaerobacterium AK17 for more efficient ethanol production. The resulting strain AK17_M6 could efficiently ferment brown seaweed hydrolysate and has the potential for ethanol production from both second and third generation biomass.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Siri Norlander, Andrius Jasilionis, Zubaida Gulshan Kazi Ara, Carl Grey, Patrick Adlercreutz, Eva Nordberg Karlsson
Summary: This study identified and characterized a novel GH5_34 subfamily arabinoxylanase, HhXyn5A, which can solubilize potential prebiotic fibers and create novel food products with increased nutritional value in cereal processing.
Article
Biotechnology & Applied Microbiology
Antoine Moenaert, Ana M. Lopez-Contreras, Miriam Budde, Leila Allahgholi, Xiaoru Hou, Anne-Belinda Bjerre, Johann Orlygsson, Eva Nordberg Karlsson, Olafur H. Friojonsson, Guomundur Oli Hreggviosson
Summary: This study compares the microbial production of alcohols from fermentable carbohydrates in the hydrolysate of macroalgae. Different microorganisms were tested for their fermentation properties, and it was found that they showed different characteristics and product profiles. The study provides important information for selecting efficient microbes for bioconversion into biofuels and chemical products.
FERMENTATION-BASEL
(2023)
