BAP1 is phosphorylated at serine 592 in S-phase following DNA damage

The human BAP1 deubiquitinating enzyme is a chromatin-bound transcriptional regulator and tumor suppressor. BAP1 functions in suppressing cell proliferation, yet its role in the DNA damage response pathway is less understood. In this study we characterized DNA damage-induced phosphorylation of BAP1 at serine 592 (pS592) and the cellular outcomes of this modification. In contrast to the majority of BAP1, pS592-BAP1 is predominantly dissociated from chromatin. Our findings support a model whereby stress induced phosphorylation functions to displace BAP1 from specific promoters. We hypothesize that this regulates the transcription of a subset of genes involved in the response to DNA damage. Structured summary of protein interactions: HCF-1 physically interacts with Bap1 by anti bait coimmunoprecipitation (1, 2) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.