Article
Engineering, Biomedical
Zejian Xu, Guancheng Shan, Nairong Hao, Lianwei Li, Tian Lan, Yabo Dong, Jiayu Wen, Ran Tian, Yan Zhang, Lianzhou Jiang, Xiaonan Sui
Summary: This study presents a strategy to tailor soy protein-derived amyloid fibrils (SAFs) into hydrogels using (-)-epigallocatechin-3-gallate (EGCG). EGCG can cleave SAFs and induce the formation of branched chains, leading to gel-like structures. SAFs-EGCG complexes show superior antibacterial activity and are non-toxic to human cells.
Article
Immunology
Yucheol Cheong, Minjin Kim, Jina Ahn, Hana Oh, Jongkwan Lim, Wonil Chae, Seung Won Yang, Min Seok Kim, Ji Eun Yu, Sanguine Byun, Yo Han Jang, Baik Lin Seong
Summary: The study investigated the potential use of catechins derived from green tea as adjuvants for subunit and inactivated vaccines, demonstrating their ability to enhance immune responses and provide protection against vaccines, including influenza virus.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Victor Valverde-Salazar, Daniel Ruiz-Gabarre, Vega Garcia-Escudero
Summary: Alzheimer's disease (AD) is a common form of dementia characterized by memory decline, cognitive impairment, and several pathological changes in the brain. Oxidative stress and inflammation play important roles in the development of AD. Green tea and its active compound EGCG have shown potential therapeutic effects in modulating AD through their antioxidative and anti-inflammatory properties. EGCG can regulate inflammatory processes, reduce protein aggregation, and promote neuronal survival pathways, making it a suitable candidate for the treatment of neurodegenerative disorders involving oxidative stress and inflammation.
Article
Engineering, Biomedical
Xiaoding Lin, Wei Liu, Xiaoyan Dong, Yan Sun
Summary: This study synthesized epigallocatechin gallate-derived carbonized polymer dots (E-CPDs) for the first time and demonstrated their multifunctional capability in inhibiting and disaggregating A,B fibrils, detecting amyloid plaques, and scavenging intracellular reactive oxygen species. The findings suggest that E-CPDs have great potential in the prevention and treatment of Alzheimer's disease (AD).
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Pengpeng Zhang, Wei Wu, Chunyu Du, Xiang Ji, Yaling Wang, Qiu Han, Hiaxia Xu, Cencen Li, Yongjie Xu
Summary: In this study, primary WAT and BAT SVF cells were isolated and induced to adipogenic differentiation in the presence or absence of EGCG. RNA-seq was used to analyze the gene regulation by EGCG in these two distinct adipose tissues and identify the key differences. The findings of this study have significant implications for obesity-related clinical and basic research.
Article
Chemistry, Applied
Jianyong Zhang, Hongchun Cui, Jiahuan Qiu, Xiaoqing Wang, Yixin Zhong, Caiping Yao, Lanying Yao, Qunxiong Zheng, ChunHua Xiong
Summary: This study aimed to improve the stability and bioavailability of EGCG in aqueous solutions and in vivo by preparing EGCG glycosylated arachin nanoparticles and EGCG glycosylated casein nanoparticles. The results showed that the DPPH scavenging activity of the nanoparticles was stable within temperature range of 25-70 degrees Celsius. EGCG enhanced the crosslinking effect of molecular particles in the glycosylation complexes solution. The glycosylated protein nanoparticles were stable to acid-base and enzymolysis in simulated gastrointestinal fluid.
Article
Multidisciplinary Sciences
Mantas Ziaunys, Kamile Mikalauskaite, Andrius Sakalauskas, Vytautas Smirnovas
Summary: The fluctuation in solution ionic strength has a significant impact on the inhibitory effect of the protein aggregation inhibitor, epigallocatechin-3-gallate, with different amyloid-forming proteins exhibiting unique responses.
Article
Biochemistry & Molecular Biology
Paulina Aguilera, Camilo Berrios-Pasten, Marcelo Veloso, Matias Galvez-Silva, Florian Turbant, Rosalba Lagos, Frank Wien, Veronique Arluison, Andres E. Marcoleta
Summary: Epigallocatechin gallate (EGCG), a green tea flavonoid, can interfere with the formation of MccE492 amyloid fibers, resulting in the formation of amorphous aggregates and small oligomers. Although the aggregation process is not completely prevented, EGCG is still an interesting inhibitor of MccE492 and other bacterial amyloids.
Article
Plant Sciences
Qianjin Xie, Xiaqiang Cai, Xu Dong, Ying Wang, Minghui Sun, Lingling Tai, Yan Xu
Summary: The combination of EGCG with Vc and glycerol could effectively increase its stability and uric acid-lowering activity, which sheds light on the development and application of EGCG in the fields of food and medicine.
PHARMACEUTICAL BIOLOGY
(2021)
Article
Chemistry, Applied
Xin Luo, Jiankang Lu, Yongyan Wu, Wenshan Duan, Fengping An, Qun Huang, Lei Chen, Shaofeng Wei
Summary: The potential allergenicity of amandin was reduced by binding it with EGCG using different methods, such as alkaline, free radical, ultrasound-assisted alkaline, and ultrasound-assisted free radical methods. The binding of amandin to EGCG was found to be covalent or non-covalent, and was mainly through cysteine and glutamate residues. This study provides a new method and theoretical basis for developing hypoallergenic almond food.
Article
Chemistry, Applied
Qing-Qing Ye, Gen-Sheng Chen, Weichun Pan, Qing-Qing Cao, Liang Zeng, Jun-Feng Yin, Yong-Quan Xu
Summary: The study focused on the contribution of EGCG to astringency, the impact of mucin as a protein, and developed a predictive model to estimate the intensity of EGCG astringency.
Article
Biochemistry & Molecular Biology
Adriana Elena Bulboaca, Alina Silvia Porfire, Vasile Rus, Cristina Ariadna Nicula, Corneliu Angelo Bulboaca, Sorana D. Bolboaca
Summary: This study aimed to evaluate the effect of liposomal epigallocatechin-gallate (LEGCG) compared with epigallocatechin-gallate (EGCG) solution on hepatic toxicity induced by gentamicin administration in rats. The results showed that LEGCG had better efficacy than EGCG in improving the oxidant/antioxidant balance, reducing inflammation, and protecting the liver from gentamicin-induced damage.
Article
Food Science & Technology
Kumju Youn, Chi-Tang Ho, Mira Jun
Summary: Alzheimer's disease is characterized by cognitive decline and memory impairment, with amyloid peptide generation and aggregation playing a key role in its pathogenesis. (-)-Epigallocatechin-3-gallate (EGCG) has been shown to have multiple neuroprotective effects in AD, focusing on amyloid peptide-induced damage and regulation.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Agriculture, Multidisciplinary
Han Peng, Fereidoon Shahidi
Summary: In this study, ester derivatives of epigallocatechin gallate (EGCG) were synthesized and their antioxidant efficacy was evaluated. The results showed that the lipophilicity of the EGCG derivatives increased with the increasing chain length of the acyl group, which affected their antioxidant performance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Qiang Peng, Ruoping Wang, Qian Zhang, Honghan Yuan, Xiaofang Luo, Wanzhen Lin, Junpeng Shi, Liang Song, Maochun Hong, Yun Zhang
Summary: The emergence of multidrug-resistant bacteria poses a serious threat to human health, and developing new antibacterial strategies is crucial. Recently, a pH-responsive targeted nanomaterial called ZEI@GCS has shown great potential in improving the bioavailability of the antibacterial agent EGCG. It also allows for evaluating the therapeutic outcome in mice.
ACS MATERIALS LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Luis F. Maia, Aleksandra Maceski, Isabel Conceicao, Laura Obici, Rui Magalhaes, Andreas Cortese, David Leppert, Giampaolo Merlini, Jens Kuhle, Maria Joao Saraiva
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2020)
Article
Oncology
Christoph Niemietz, Filipa Bezerra, Maria Rosario Almeida, Shuling Guo, Brett P. Monia, Maria Joao Saraiva, Paula Schuetz, Hartmut H-J Schmidt, Andree Zibert
EXPERIMENTAL CELL RESEARCH
(2020)
Review
Neurosciences
Filipa Bezerra, Maria Joao Saraiva, Maria Rosario Almeida
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2020)
Article
Immunology
Joao Moreira, Susete Costelha, Margarida Saraiva, Maria Joao Saraiva
Summary: Inflammation is a key feature of neurodegenerative disorders like ATTRv. Compared to wild type animals, chemokine expression is significantly downregulated in the peripheral nervous system of a mouse model of the disease, possibly due to a mechanism that leads to the inactivation of TTR activation in patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Filipa Bezerra, Christoph Niemietz, Hartmut H. J. Schmidt, Andree Zibert, Shuling Guo, Brett P. Monia, Paula Goncalves, Maria Joao Saraiva, Maria Rosario Almeida
Summary: The SerpinA1 plays an important role in regulating the proteolysis and aggregation of transthyretin. Downregulation of SerpinA1 leads to increased TTR levels in mice plasma and deposition in the cardiac tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Aubin Michalon, Andreas Hagenbuch, Christian Huy, Evita Varela, Benoit Combaluzier, Thibaud Damy, Ole B. Suhr, Maria J. Saraiva, Christoph Hock, Roger M. Nitsch, Jan Grimm
Summary: Through analyzing memory B cell repertoires of healthy elderly, researchers developed a selective human antibody that removes cardiac amyloid by recruiting phagocytic immune cells.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Tiago Giao, Tiago Teixeira, Maria Rosario Almeida, Isabel Cardoso
Summary: The choroid plexus is a monolayer of epithelial cells in the brain ventricles that forms a blood-cerebrospinal fluid barrier. It secretes cerebrospinal fluid and plays a role in immune surveillance and toxin removal. Impaired function of the choroid plexus is associated with diseases like Alzheimer's, and therapies to restore its function are being proposed.
Article
Chemistry, Medicinal
Francisca Pinheiro, Irantzu Pallares, Francesca Peccati, Adria Sanchez-Morales, Nathalia Varejao, Filipa Bezerra, David Ortega-Alarcon, Danilo Gonzalez, Marcelo Osorio, Susanna Navarro, Adrian Velazquez-Campoy, Maria Rosario Almeida, David Reverter, Felix Busque, Ramon Alibes, Mariona Sodupe, Salvador Ventura
Summary: This study discovered a series of novel halogenated kinetic stabilizers for transthyretin (TTR) through rational design and molecular dynamics (MD) simulations. Among them, M-23 displayed high affinity for TTR and demonstrated enhanced tetramer stability. The crystal structure confirmed the unprecedented protein-ligand contacts predicted by MD simulations.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Clinical Neurology
Joao R. Gomes, Andrea Lobo, Renata Nogueira, Ana F. Terceiro, Susete Costelha, Igor M. Lopes, Ana Magalhaes, Teresa Summavielle, Maria J. Saraiva
BRAIN COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Merrill D. Benson, Joel N. Buxbaum, David S. Eisenberg, Giampaolo Merlini, Maria J. M. Saraiva, Yoshiki Sekijima, Jean D. Sipe, Per Westermark
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Carlos J. V. Simoes, Zaida L. Almeida, Ana L. Cardoso, Filipa Bezerra, Maria R. Almeida, Joao Beirao, Teresa M. V. D. Pinho e Melo, Maria J. Saraiva, Rui M. M. Brito
AMYLOID-JOURNAL OF PROTEIN FOLDING DISORDERS
(2019)