期刊
FEBS LETTERS
卷 586, 期 19, 页码 3522-3528出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.08.012
关键词
ARHGAP21; Proliferation; SUMO2/3; Post-translational modification
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [101624/2011-5]
- Fundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) [09/07322-8, 07/54870-5, 07/08019-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/54870-5, 07/08019-1] Funding Source: FAPESP
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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