期刊
FEBS LETTERS
卷 585, 期 16, 页码 2575-2581出版社
WILEY
DOI: 10.1016/j.febslet.2011.06.038
关键词
DENV; Core protein; NS5; NS3 helicase; RNA chaperone; Strand annealing; RNA binding protein
资金
- National Science Council [NSC-98-2320-B-001-013-MY3]
- National Health Research Institute [NHRI-EX99-9745SI]
In this study we showed that the dengue virus (DENV) core protein forms a dimer with an alpha-helix-rich structure, binds RNA and facilitates the strand annealing process. To assess the RNA chaperone activity of this core protein and other dengue viral RNA-interacting proteins, such as NS3 helicase and NS5 proteins, we engineered cis- and trans-cleavage hammerhead ribozyme constructs carrying DENV genomic RNA elements. Our results indicate that DENV core protein facilitates typical hammerhead structure formation by acting as an RNA chaperone and DENV NS5 has a weak RNA chaperone activity, while DENV NS3 helicase failed to refold RNA with a complex secondary structure. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据