Article
Nanoscience & Nanotechnology
Yuto Honda, Takahiro Nomoto, Makoto Matsui, Hiroyasu Takemoto, Yutaka Miura, Nobuhiro Nishiyama
Summary: The construction of a systemically applicable beta-Gal-loaded ternary complex through sequential self-assembly in aqueous solution was reported. The complex protected the loaded beta-Gal from degradation and exhibited enhanced and selective enzymatic activity at the tumor site. This novel approach may offer a new strategy for enzyme-prodrug theragnosis.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Qianqian Shi, Zijun Yong, Md Hemayet Uddin, Runfang Fu, Debabrata Sikdar, Lim Wei Yap, Bo Fan, Yiyi Liu, Dashen Dong, Wenlong Cheng
Summary: Tissues consist of closely packed cell arrays, resembling sheet-like biosynthesis plants. Soft polystyrene-encased nanoframe reactor arrays can synthesize novel nanocrystals in a unique confined environment, enabling directional inward crystallization.
ADVANCED MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Weicai Wang, Yuanyuan Wang, Minghui Ma, Hyung Jong Jin, Xuemei Li
Summary: A cancer-targeted cascade bioreactor was constructed by inserting glucose oxidase, catalase, and chlorin e6 into human serum albumin assembly molecules, which promoted oxygen production and cytotoxic singlet oxygen generation in cancer cells, enhancing the efficacy of photodynamic therapy and chemotherapy.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yuanzhi Zhong, Jie Zhan, Guanghui Xu, Yumiao Chen, Qin Qin, Xu Liao, Shaodan Ma, Zhimou Yang, Yanbin Cai
Summary: The study demonstrates the construction of high-performing excimer-based luminescent tools at low molecular concentrations using enzyme-instructed self-assembly. The luminescent supramolecular nanofibers show potential for early cancer diagnosis and expand the application of supramolecular chemistry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Pharmacology & Pharmacy
Debin Zheng, Jingfei Liu, Limin Xie, Yuhan Wang, Yinghao Ding, Rong Peng, Min Cui, Ling Wang, Yongjie Zhang, Chunqiu Zhang, Zhimou Yang
Summary: An enzymatic self-assembly strategy using the peptide F-pY-T was developed to induce mitochondrial oxidative stress in cancer cells, leading to immunogenic cell death (ICD) and enhanced immune responses. Combination therapy with anti-PD-L1 agents further improved the immunotherapeutic capacity without biotoxicity in major organs.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Xianbao Sun, Manli Wang, Xiaoyang Liu, Wenjun Zhan, Guangqiong Xu, Qiaochu Jiang, Hai-Dong Xu, Fuqiang Wang, Gaolin Liang
Summary: This study proposed an enzymatic self-assembly/disassembly strategy to regulate the mimetic hydrolase activities. By designing a peptide molecule and reacting it with phosphatase or kinase, the activity of the artificial enzyme could be turned ON or OFF. This work provides a feasible strategy for the fabrication of artificial enzymes with controllable and superior activities.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xiaomin Qian, Isabella Nymann Westensee, Catarina Cavalheiro Fernandes, Brigitte Stadler
Summary: This study demonstrates the use of artificial enzymes to facilitate signal transfer between artificial cells and mammalian cells, opening up new opportunities for integrating the synthetic and living worlds with a bottom-up strategy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Qing Li, Jiwei Min, Jiaxing Zhang, Meital Reches, Yuhe Shen, Rongxin Su, Yuefei Wang, Wei Qi
Summary: Inspired by the regulation of reactive-site phosphorylation, researchers developed peptide-based catecholase mimetics with switchable catalytic activity and high selectivity through the co-assembly of nanofibers comprising peptides and copper ions. Kinase/phosphatase switches were used to control the reversible transition of nanofiber formation and depolymerization, as well as to modulate the active-site microenvironment. The study provides novel avenues for designing biomimetic enzymes with ordered structures and dynamic catalytic activities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Yaoxia Chen, Wenwen Zhang, Yinghao Ding, Chunhui Liang, Yang Shi, Zhi-Wen Hu, Ling Wang, Zhimou Yang
Summary: In this study, the researchers used phosphatase-based enzyme-instructed self-assembly to monitor the final structures and catalytic activities of artificial esterases. Peptide precursors with different phosphorylation sites could preorganize into alternating nanostructures and undergo dephosphorylation in the presence of alkaline phosphatase. Predecessors with enhanced preorganized states were more effective in promoting dephosphorylation and enhancing catalytic activities.
SCIENCE CHINA-CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Xiangyi Wang, Yi Jiang, Hongling Liu, Xinyi Zhang, Haibo Yuan, Di Huang, Tengfei Wang
Summary: This study presents a novel approach utilizing a scaffold protein-mediated assembly method for the formation of a trehalose bi-enzyme complex and explores strategies to enhance enzyme utilization through the construction of a bi-enzyme complex under optimal conditions in vitro. The results demonstrate that the bi-enzyme complex showed higher trehalose production levels compared to free enzyme mixtures, with a sustained upward trend.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Basudev Maity, Mohd Taher, Shyamalava Mazumdar, Takafumi Ueno
Summary: Metalloenzymes play vital roles in biology, and artificial metalloenzymes use synthetic metal cofactors to promote non-natural reactions. Significant progress has been made in manipulating artificial metalloenzymes for various organic transformation reactions, such as C-H activation and C-C coupling. Advanced methods like Directed evolution, high throughput screening, and rational design have driven the research of artificial metalloenzymes. The applications of artificial metalloenzymes have expanded to control cellular functions such as prodrug activation. However, the study of artificial metalloenzymes in more complex processes and reaction environments is relatively limited.
COORDINATION CHEMISTRY REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Zhen Luo, Yujuan Gao, Zhongyu Duan, Yu Yi, Hao Wang
Summary: Mitochondria play a vital role in cancer therapy, with ongoing research focusing on targeted drug development for mitochondria. Particularly, the in situ self-assembling peptide-nanomaterials are considered as a promising technology with great potential for application in cancer treatments.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Lei Jiang, Yaning Han, Susana Fernandez-Garcia, Miguel Tinoco, Zhuang Li, Pengli Nan, Jingtao Sun, Juan J. Delgado, Huiyan Pan, Ginesa Blanco, Javier Martinez-Lopez, Ana B. Hungria, Jose J. Calvino, Xiaowei Chen
Summary: The ability to mimic protein-based oxidase with multi-functional inorganic nanozymes would greatly advance biomedical and clinical practices. Praseodymia (PrOx) nanorods (NRs) and nanoparticles (NPs) with significantly higher oxidase-like activities have been synthesized. One of the substrates, dopamine, can be oxidized and further polymerized to generate polydopamine. Different mechanisms involving holes+, oxygen vacancies and oxygen mobility over PrOx catalysts have been proposed, and fluoride ions were found to impose opposite effects on the oxidase-mimicking activity of PrOx and CeO2.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Mohd Taher, Basudev Maity, Taiki Nakane, Satoshi Abe, Takafumi Ueno, Shyamalava Mazumdar
Summary: The study shows that mutations at the core of the ferritin nanocage can impact the uptake of IrCp*, with mutation of Arg52 by His leading to a significant increase in uptake. Additionally, the ferritin variants were found to exhibit high activity and enantioselectivity in catalyzing transfer hydrogenation reactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Qinglong Qiao, Wenjuan Liu, Jie Chen, Xia Wu, Fei Deng, Xiangning Fang, Ning Xu, Wei Zhou, Shaowei Wu, Wenting Yin, Xiaogang Liu, Zhaochao Xu
Summary: This study presents a self-blinking fluorophore that achieves high-resolution imaging under acidic lysosomal conditions. The fluorophore is sensitive to lysosomal pH, enabling simultaneous dynamic and pH recognition of lysosomes at the single-lysosome level. The probe allows for the characterization of lysosomal subpopulations based on distribution, size, and luminal pH, as well as the observation of various lysosome movement trajectories and interaction modes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Pharmacology & Pharmacy
Patricia Figueiredo, Vimalkumar Balasubramanian, Mohammad-Ali Shahbazi, Alexandra Correia, Dalin Wu, Cornelia G. Palivan, Jouni T. Hirvonen, Helder A. Santos
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2016)
Article
Engineering, Biomedical
Zehua Liu, Vimalkumar Balasubramanian, Chinmay Bhat, Mikko Vahermo, Ermei Makila, Marianna Kemell, Flavia Fontana, Agne Janoniene, Vilma Petrikaite, Jarno Salonen, Jari Yli-Kauhaluoma, Jouni Hirvonen, Hongbo Zhang, Helder A. Santos
ADVANCED HEALTHCARE MATERIALS
(2017)
Article
Pharmacology & Pharmacy
Nayab Tahir, Asadullah Madni, Vimalkumar Balasubramanian, Mubashar Rehman, Alexandra Correia, Prince Muhammad Kashif, Ermei Makila, Jarno Salonen, Helder A. Santos
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2017)
Article
Chemistry, Multidisciplinary
Monica P. A. Ferreira, Sanjeev Ranjan, Sini Kinnunen, Alexandra Correia, Virpi Talman, Ermei Makila, Brianda Barrios-Lopez, Marianna Kemell, Vimalkumar Balasubramanian, Jarno Salonen, Jouni Hirvonen, Heikki Ruskoaho, Anu J. Airaksinen, Helder A. Santos
Article
Engineering, Biomedical
Vimalkumar Balasubramanian, Zehua Liu, Jouni Hirvonen, Helder A. Santos
ADVANCED HEALTHCARE MATERIALS
(2018)
Article
Materials Science, Biomaterials
Vimalkumar Balasubramanian, Andrea Poillucci, Alexandra Correia, Hongbo Zhang, Christian Celia, Helder A. Santos
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Materials Science, Biomaterials
Vimalkumar Balasubramanian, Andrea Poillucci, Alexandra Correia, Hongbo Zhang, Christian Celia, Helder A. Santos
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Nanoscience & Nanotechnology
Nayab Tahir, Asadullah Madni, Alexandra Correia, Mubashar Rehman, Vimalkumar Balasubramanian, Muhammad Muzamil Khan, Helder A. Santos
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2019)
Article
Engineering, Biomedical
Vimalkumar Balasubramanian, Andrii Domanskyi, Juho-Matti Renko, Mirkka Sarparanta, Chang-Fang Wang, Alexandra Correia, Ermei Makila, Osku S. Alanen, Jarno Salonen, Anu J. Airaksinen, Raimo Tuominen, Jouni Hirvonen, Mikko Airavaara, Helder A. Santos
Article
Engineering, Biomedical
Surachet Imlimthan, Alexandra Correia, Patricia Figueiredo, Kalle Lintinen, Vimalkumar Balasubramanian, Anu J. Airaksinen, Mauri A. Kostiainen, Helder A. Santos, Mirkka Sarparanta
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Chemistry, Multidisciplinary
Giulia Torrieri, Monica P. A. Ferreira, Mohammad-Ali Shahbazi, Virpi Talman, S. Tuuli Karhu, Lotta Pohjolainen, Claudia Carvalho, Joao F. Pinto, Jouni Hirvonen, Heikki Ruskoaho, Vimalkumar Balasubramanian, Helder A. Santos
Summary: The developed nanosystem has the capability to stimulate cardiomyocyte proliferation and reduce fibrosis by approximately 80%, showing potential benefits for future in vivo applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Physical
Voitto Kankanen, Micaela Fernandes, Zehua Liu, Jani Seitsonen, Sami-Pekka Hirvonen, Janne Ruokolainen, Joao F. Pinto, Jouni Hirvonen, Vimalkumar Balasubramanian, Helder A. Santos
Summary: This study reports the development of poly(ethylene glycol)-block-(e-caprolactone) (PEG-b-PCL) nanoparticles using a microfluidic technique for intravenous drug delivery. The optimized formulation achieved spherical morphology, small particle size (dH < 80 nm), uniform size distribution (PDI < 0.2), high drug loading degree (16%), and encapsulation efficiency of 54%. Furthermore, the nanoparticles exhibited stability and sustained release of sorafenib in vitro over several days. Overall, this research demonstrates the use of microfluidic approach in optimizing drug delivery systems.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Voitto Kankanen, Jani Seitsonen, Henri Tuovinen, Janne Ruokolainen, Jouni Hirvonen, Vimalkumar Balasubramanian, Elder A. Santos
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Chemistry, Multidisciplinary
Giulia Torrieri, Flavia Fontana, Patricia Figueiredo, Zehua Liu, Monica P. A. Ferreira, Virpi Talman, Joao P. Martins, Manlio Fusciello, Karina Moslova, Tambet Teesalu, Vincenzo Cerullo, Jouni Hirvonen, Heikki Ruskoaho, Vimalkumar Balasubramanian, Helder A. Santos
Article
Chemistry, Multidisciplinary
Vimalkumar Balasubramanian, Alexandra Correia, Hongbo Zhang, Flavia Fontana, Ermei Makila, Jarno Salonen, Jouni Hirvonen, Helder A. Santos
ADVANCED MATERIALS
(2017)