期刊
FEBS LETTERS
卷 585, 期 17, 页码 2682-2687出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2011.07.047
关键词
Ago2; MicroRNA; NMD; CBP80/20; eIF4E
资金
- Korea government (MEST) [2009-0084897, 0001197]
- Korean Government (MOEHRD) [KRF-2008-314-C00247]
- National Research Foundation of Korea [2009-0084897] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Tethering of Ago2, but not of Ago2F2V2 which lacks cap -association activity, to the 3'UTR of PTC-containing mRNA abrogates NMD. Immunoprecipitation using CBP80 antibody reveals that Ago2, but not Ago2F2V2, inhibits the binding of CBP80/20 to cap structure. Our observations provide molecular insight into the cross-talk between miRNA-mediated gene silencing, CT, and NMD. Structured summary of protein interactions: AGO2 physically interacts with GW182 by anti tag coimmunoprecipitation (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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