期刊
FEBS LETTERS
卷 585, 期 5, 页码 791-796出版社
WILEY
DOI: 10.1016/j.febslet.2011.02.002
关键词
Ran binding protein 1 (RanBP1); Ran binding protein 2 (RanBP2); RanGTPase activity
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Takeda Science Foundation
The small GTPase Ran plays important roles in multiple aspects of cellular function. Maximal RanGAP activity is achieved with the aid of RanBP1 and/or presumably of RanBP2. Here, we show that RanBP1-knockout mice are unexpectedly viable, and exhibit male infertility due to a spermatogenesis arrest, presumably caused by down-regulation of RanBP2 during spermatogenesis. Indeed, siRNA-mediated depletion of RanBP2 caused severe cell death only in RanBP1-deficient MEFs, indicating that simultaneous depletion of RanBP1 and RanBP2 severely affects normal cell viability. Collectively, we conclude that the dramatic decrease in RanBP activity impairs germ cell viability and affects spermatogenesis decisively in RanBP1-knockout mice. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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