Review
Biochemistry & Molecular Biology
Laura Brohee, Julie Cremer, Alain Colige, Christophe Deroanne
Summary: The rewiring of lipid metabolism in cancer cells is associated with their increased aggressiveness, making the targeting of lipid metabolism an appealing therapeutic strategy. Lipin-1, a central regulator of lipid homeostasis, has been highlighted for its potential role in cancer progression, both in cancer cells and the tumor microenvironment. Inhibiting Lipin-1 may serve as an adjuvant therapy to enhance the effects of anti-cancer treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Weijing Gu, Shujuan Gao, Huan Wang, Kaelin D. Fleming, Reece M. Hoffmann, Jong Won Yang, Nimi M. Patel, Yong Mi Choi, John E. Burke, Karen Reue, Michael Airola
Summary: This study elucidates the structural basis of lipin membrane association and identifies a crucial lipin domain with a novel protein fold that is essential for membrane binding and full functionality of lipins.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Kari T. Chambers, Michael A. Cooper, Alison R. Swearingen, Rita T. Brookheart, George G. Schweitzer, Carla J. Weinheimer, Attila Kovacs, Timothy R. Koves, Deborah M. Muoio, Kyle S. McCommis, Brian N. Finck
Summary: Loss of Lipin 1 impairs cardiac functional reserve through effects on glycerolipid homeostasis, mitochondrial function, and protein kinase A signaling.
Review
Biochemistry & Molecular Biology
Karin Athenstaedt
Summary: Phosphatidic acid biosynthesis is the key process in glycerolipid metabolism, involving two consecutive acylation reactions. Yeast and mammals have additional pathways for phosphatidic acid synthesis. Further research is needed to understand this biosynthesis process and the entire lipid metabolic network.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Rui Kamada, Sae Uno, Nozomi Kimura, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
Summary: This study demonstrates the important role of PPM1D phosphatase in regulating lipid droplet (LD) formation in mature adipocytes. PPM1D controls LD size by dephosphorylating Ser511 of perilipin 1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Usha Pallabi Kar, Himani Dey, Abdur Rahaman
Summary: This study reveals the mechanism of nuclear membrane recruitment of Drp6 in Tetrahymena thermophila. Drp6 recruitment depends on its membrane-binding domain that interacts with cardiolipin-rich bilayers, and its nuclear localization is affected by cellular cardiolipin levels.
Article
Plant Sciences
Ana Carolina Vilchez, Micaela Peppino Margutti, Matias Reyna, Natalia Wilke, Ana Laura Villasuso
Summary: The study compared the lipid profile in barley roots under chilling stress and subsequent recovery, revealing that lipid metabolism and editing were affected by cold stress, with acyl-editing of phosphatidic acid possibly being a regulatory mechanism for cold tolerance in barley.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yeun Ju Kim, Nivedita Sengupta, Mira Sohn, Amrita Mandal, Joshua G. Pemberton, Uimook Choi, Tamas Balla
Summary: This study reveals that the metabolic fate of newly synthesized phosphatidic acid (PA), the lipid precursor of phosphatidylinositol (PI), is influenced by the fatty acyl-CoA used, leading to preferential routing of the arachidonoyl-enriched species towards PI synthesis. Additionally, the unsaturated forms of PI(4,5P)(2) are replenished faster than the saturated forms during PLC activation, indicating a favored recycling of the unsaturated forms of the PLC-generated hydrolytic products. CDS2 contributes to increased PI resynthesis during PLC activation, while Nir2 contributes to rapid PPIn resynthesis, but the faster re-synthesis of the 38:4 species does not depend on Nir2. Therefore, the fatty acid side-chain composition of the lipid precursors used for PI synthesis is an important determinant of their metabolic fates and the maintenance of the unique fatty acid profile of PPIn lipids.
Review
Plant Sciences
Parul Sharma, Nita Lakra, Alisha Goyal, Yogesh K. Ahlawat, Abbu Zaid, Kadambot H. M. Siddique
Summary: Lipids play a crucial role in protecting plant cells and mediating responses to abiotic stress. Recent studies have shown that lipids can act as signal mediators in reducing stress responses and activating defense systems in plants. This review focuses on the synthesis of various signaling lipids and their roles in abiotic stress tolerance, providing valuable insights for further investigation into the interactions between plant lipids and abiotic stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Changcheng Xu, Jilian Fan
Summary: This review discusses the metabolic interplay between autophagy and lipid droplet dynamics, mechanisms of lipophagy, and cellular management of lipotoxic stress during autophagy. Autophagy degrades membrane lipids to provide fatty acids for energy production, while lipid droplets can be degraded via lipophagy in times of energy scarcity. However, lipid droplets are also necessary for efficient autophagic processes, and cells must carefully manage the cytotoxic effects of free fatty acids during autophagy-mediated mobilization for energy generation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Nutrition & Dietetics
Yangjun Yang, Xi Li, Zonghan Liu, Xinyu Ruan, Huihui Wang, Qiang Zhang, Lu Cao, Luchen Song, Yinghong Chen, Yi Sun
Summary: Moderate treadmill exercise helps regulate lipid droplet dynamics caused by non-alcoholic fatty liver disease (NAFLD) and improves the condition by inhibiting abnormal lipid droplet expansion and enhancing clearance of the droplets.
Article
Biochemistry & Molecular Biology
Shuang Guo, Zhong Tian, Fen Zhu, Wen Liu, Xiao-Ping Wang
Summary: This study examined the role of lipin protein in the reproductive process of female cabbage beetles. It was found that lipin plays a vital role in maintaining lipid homeostasis, promoting ovarian development, and regulating lipid synthesis in the beetles. These findings provide a foundation for future research on the relationship between lipid metabolism and reproduction in invertebrates.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hejiang Zhou, Yanwu Huo, Na Yang, Taotao Wei
Summary: Phosphatidic acid (PA) is a key metabolic intermediate and second messenger that influences cellular and physiological processes across various species. PA levels change dynamically in response to stimuli and can be produced and degraded by multiple enzymatic reactions. PA acts as a signalling molecule, regulating cellular processes through its effects on membrane tethering, target protein enzymatic activities, and vesicular trafficking. Its unique physicochemical properties make PA a class of new lipid mediators that impact membrane structure, dynamics, and protein interactions.
Review
Plant Sciences
Yingqi Cai, Xiao-Hong Yu, John Shanklin
Summary: Plants convert carbohydrates into energy-dense lipids through photosynthesis, making plant oils an economically and sustainably attractive feedstock for biofuels and value-added bioproducts. Various strategies have been developed to enhance lipid accumulation and produce specialty lipids with increased uses and value. Successful metabolic engineering involves expression of lipogenic factors from other sources or with specialized functionality.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Liang Huang, Qichao Liao, Tingli Pan, Yu Sun, Zhier Aluo, Lianggui Xiao, Jingsu Yu, Siqi Liu, Yang Xiao, Yufeng Yang, Yixing Li, Lei Zhou
Summary: This study highlights the importance of CIDEC in lipid metabolism, particularly in the small intestine. Knockout mice with a specific deletion of CIDEC in the small intestine showed improved lipid metabolism, including decreased body weight, body fat mass, and liver triglycerides. Mechanistic studies revealed that CIDEC interacts with 1-acylglycerol-3-phosphate-O-acyltransferase to promote phosphatidic acid synthesis and TG accumulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hamideh Ghazizadeh, Mary Kathryn Bohn, Mahdiyeh Yaghooti-Khorasani, Roshanak Ghaffarian-Zirak, Mohsen Valizadeh, Maryam Saberi-Karimian, Hamideh Safarian, Atieh Kamel-Khodabandeh, Reza Zare-Feyzabadi, Ameneh Timar, Maryam Mohammadi-Bajgiran, Mohammad Reza Oladi, Meysam Gachpazan, Mohadeseh Rohban, Habibollah Esmaily, Gordon A. Ferns, Khosrow Adeli, Majid Ghayour-Mobarhan
Summary: The study found that there were no significant age and sex-specific differences in most biomarkers, except for uric acid which had higher concentrations in men. Only hs-CRP levels were positively associated with higher BMI among participants with a BMI above 25. Reference intervals for PAB and anti-hsp-27 in men and women aged 35-65 were reported. The partitioning by sex and BMI was only required for uric acid and hs-CRP, while other biomarkers required no partitioning.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Medical Laboratory Technology
Mary Kathryn Bohn, Peter Yousef, Shannon Steele, Lusia Sepiashvili, Khosrow Adeli
Summary: MIS-C presents unique inflammatory signatures involving adaptive and innate systems. Laboratory testing plays a crucial role in the diagnosis and monitoring of MIS-C. There are major gaps in understanding MISC pathogenesis and long-term health outcomes.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Mary Kathryn Bohn, Paul Horn, Donna League, Paul Steele, Alexandra Hall, Khosrow Adeli
Summary: The current study established pediatric reference intervals for 32 analytes using Siemens Healthineers Atellica (R) CH assays in healthy children and adolescents of the CALIPER cohort. The study found consistent increases, decreases, and dynamic variations across the age continuum for most parameters, with few exceptions such as lipase and bilirubin. The reference value distributions for Siemens assays were generally in line with other comparable assays/systems, supporting the robustness of derived reference intervals in the CALIPER cohort and other global cohorts.
CLINICAL BIOCHEMISTRY
(2022)
Review
Medical Laboratory Technology
Giuseppe Lippi, Brandon M. Henry, Khosrow Adeli
Summary: This article presents the first pooled analysis of the diagnostic accuracy of the fully-automated Roche Elecsys severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen electrochemiluminescence immunoassay (ECLIA) for diagnosing COVID-19. The results indicate that the ECLIA has high diagnostic specificity and optimal diagnostic sensitivity for identifying nasopharyngeal samples with higher viral load. It is a reliable technique for mass screening and for supporting strategies based on shorten isolation and/or quarantine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Ruth Ronn, Mary Kathryn Bohn, Ellen M. Greenblatt, Barry Hoffman, Khosrow Adeli
Summary: This study aims to establish accurate reference intervals for anti-mullerian hormone (AMH) in pediatric/adolescent survivors to assess their reproductive potential. The study found differences in AMH concentrations among females of different age groups but these differences were unlikely to be clinically meaningful. Menstrual status and ethnicity did not significantly impact AMH concentrations.
CLINICAL BIOCHEMISTRY
(2022)
Article
Medical Laboratory Technology
Mary Kathryn Bohn, Siobhan Wilson, Randal Schneider, Youssef Massamiri, Edward W. Randell, Khosrow Adeli
Summary: This study verified the performance of the Alinity i assays in clinical laboratory investigation of autoimmune, metabolic, and oncologic disorders in children and adolescents. It also confirmed the transferability of the pediatric reference intervals established in the CALIPER cohort for 16 specialized immunoassays from the ARCHITECT system to the Alinity system.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Lusia Sepiashvili, Mary Kathryn Bohn, Alexandra Hall, Tina Henderson, Jack Chen, Roseline Dunst, Khosrow Adeli
Summary: The objective of this study was to establish pediatric reference limits for autoimmune disease markers in healthy children and adolescents. Serum autoantibody testing was conducted on a BIO-FLASH analyzer and reference limits were calculated using the non-parametric rank method. The results showed a proportion of samples with results above the lower limit of the analytical measuring range for each marker. The study concluded that the established reference limits will improve laboratory assessment and clinical decision making in pediatric patients using the BIO-FLASH assay platform worldwide.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Review
Microbiology
Nyan Abdalqadir, Khosrow Adeli
Summary: The intestine plays a crucial role in maintaining metabolic homeostasis, energy expenditure, and overall human health by sensing and responding to various signals, harboring a large population of microbes, and secreting a wide variety of hormones and molecules to regulate nutrition and physiology.
Letter
Cardiac & Cardiovascular Systems
Victoria Higgins, Nicole White-Al Habeeb, Khosrow Adeli
CANADIAN JOURNAL OF CARDIOLOGY
(2023)
Article
Medical Laboratory Technology
Rainer Haeckel, Khosrow Adeli, Graham Jones, Ken Sikaris, Werner Wosniok
Summary: This paper proposes a general definition covering all indirect models currently in use. With the increasing popularity of indirect models, further partitioning strategies are necessary to minimize the risk of patients' false classifications. Indirect methods make these partitions much easier to execute compared to direct methods. The authors believe that the future of reference interval estimation lies in indirect models with big data pools from either one laboratory or combined from several regional centres.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Siobhan M. Wilson, Mary Kathryn Bohn, Andre Madsen, Thomas Hundhausen, Khosrow Adeli
Summary: This study establishes continuous reference percentiles for 14 biomarkers in a healthy pediatric population using the LMS model. The LMS-based approach improves test result interpretation, especially with repeated measures over time.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Pyper Olver, Mary Kathryn Bohn, Khosrow Adeli
Summary: Clinical laboratories play a vital role in the healthcare system. The International Federation for Clinical Chemistry and Laboratory Medicine (IFCC) has established a task force to support research on the role of laboratory medicine in clinical outcomes. This review discusses the contribution of laboratory medicine in diagnosing and managing heart diseases, sepsis, traumatic brain injury, and the fight against the COVID-19 pandemic.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Cell Biology
Niloufar Abdollahian, Hamideh Ghazizadeh, Maryam Mohammadi-Bajgiran, Mehran Pashirzad, Mahdiyeh Yaghooti Khorasani, Mary Kathryn Bohn, Shannon Steele, Fatemeh Roudi, Atieh Kamel Khodabandeh, Sara Ghazi Zadeh, Iman Alami-Arani, Seyede Negin Badakhshan, Habibollah Esmaily, Gordon A. Ferns, Reza Assaran-Darban, Khosrow Adeli, Majid Ghayour-Mobarhan
Summary: Age-specific reference intervals for 7 laboratory biochemical parameters were determined in an Iranian pediatric population. These findings are valuable for the clinical interpretation of blood test results and disease management in this population.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Hematology
Mary Kathryn Bohn, Siobhan Wilson, Shannon Steele, Khosrow Adeli
Summary: The current study aimed to establish pediatric reference intervals (RIs) for both common and novel hematology parameters on the Mindray BC-6800Plus system. Dynamic reference value distributions were observed for several parameters, emphasizing the complex biological patterns of hematology parameters in childhood, particularly at the onset of puberty. These findings support the need for age- and sex-specific RIs for appropriate clinical interpretation.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Review
Medicine, General & Internal
Giuseppe Lippi, Brandon M. Henry, Khosrow Adeli, Mario Plebani
Summary: This study provides a pooled analysis of the accuracy of the Fujirebio Lumipulse SARS-CoV-2 Antigen chemiluminescent immunoassay for diagnosing acute SARS-CoV-2 infections. The analysis shows that this method has good diagnostic sensitivity and specificity, making it a valuable option for complementing and integrating with molecular testing for SARS-CoV-2 in the current pandemic.