Review
Biophysics
Judy L. M. Kotler, Timothy O. Street
Summary: The Hsp40, Hsp70, and Hsp90 chaperone families play important roles in cellular protein homeostasis. Recent studies have explored the possibility of understanding how these chaperones work together as a unified system. This review focuses on the mechanics of ERdj3 (an Hsp40), BiP (an Hsp70), and Grp94 (an Hsp90) chaperones within the endoplasmic reticulum, examining their interactions and the gaps in our understanding. Calculations are also used to investigate the impacts of client transfer on protein solubilization, protein folding, and triage decisions for protein degradation. Experimental tests of the proposed roles of client transfer among Hsp40-Hsp70-Hsp90 chaperones are discussed.
ANNUAL REVIEW OF BIOPHYSICS
(2023)
Review
Microbiology
Colin R. Harwood, Yoshimi Kikuchi
Summary: Most bacteria reproduce by binary fission and may be considered immortal, although they age due to damage to DNA and proteins. DNA damage can often be repaired, but proteins have a functional lifespan and require specific degradation processes. Proteases play crucial roles in various cellular functions in different parts of the cell.
FEMS MICROBIOLOGY REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Anna M. Lenkiewicz, Magda Krakowczyk, Piotr Bragoszewski
Summary: The synthesis and targeting of mitochondrial proteins are essential processes for maintaining mitochondrial function and cell survival. The cytosolic protein homeostasis mechanism and the ubiquitin-proteasome system play critical roles in controlling the quality and supply of mitochondrial proteins. Mislocalization of mitochondrial proteins can contribute to disease development. Pathways involved in precursor maintenance and guidance in the cytosolic stages are crucial for restoring protein homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Felicity Alcock, Ben C. Berks
Summary: This study reports the recovery of receptor complexes associated with elevated levels of TatA through detergent solubilization of membranes during active transport. By combining biochemical analysis and live cell fluorescence imaging, sub-steps in the Tat translocation cycle were resolved, and it was inferred that TatA assembly precedes the functional interaction with TatC. It was also observed that dissipation of the protonmotive force releases TatA oligomers from the assembled translocation site, suggesting that the TatA oligomer is assembled at the periphery of the receptor complex.
MOLECULAR MICROBIOLOGY
(2022)
Article
Construction & Building Technology
Feng Wang, Fan Chen, Tengfei (Tim) Zhang
Summary: This study developed an aisle displacement ventilation system that can maintain a low air velocity distribution in the cabin, reduce air temperature stratification, and effectively remove airborne contaminants. The experimental results showed that the system has better thermal comfort compared to the other two systems, but the impact on cabin air quality is similar.
BUILDING AND ENVIRONMENT
(2022)
Review
Biochemistry & Molecular Biology
Jing-Liang Sun, Jin-Yu Li, Mei-Jing Wang, Ze-Ting Song, Jian-Xiang Liu
Summary: Protein homeostasis in plant organelles is crucial for cell functions during growth and stress responses; a balance between protein synthesis and degradation is required to maintain this stability. Various mechanisms have evolved in plants to ensure efficient import, correct folding, and maturation of proteins within organelles, influenced by developmental signals and environmental cues.
Article
Biochemistry & Molecular Biology
Amanda B. Abildgaard, Vasileios Voutsinos, Soren D. Petersen, Fia B. Larsen, Caroline Kampmeyer, Kristoffer E. Johansson, Amelie Stein, Tommer Ravid, Claes Andreasson, Michael K. Jensen, Kresten Lindorff-Larsen, Rasmus Hartmann-Petersen
Summary: Protein quality control (PQC) degrons are short protein segments that target misfolded proteins for proteasomal degradation, and chaperone-binding regions may function as PQC degrons. A canonical Hsp70-binding motif, the APPY peptide, functions as a dose-dependent PQC degron in yeast and human cells. The number of exposed Hsp70-binding sites in the yeast proteome correlates with reduced protein abundance and half-life.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Chen Jiang, Max Wynne, Damon Huber
Summary: The Sec machinery in bacteria is responsible for transporting proteins across the cytoplasmic membrane, requiring the substrates to be in an unfolded conformation. To prevent folding in the cytoplasm, bacteria have evolved a complex quality control network consisting of three branches: avoidance of cytoplasmic intermediates, inhibition of folding Sec substrate proteins, and destruction of potential translocation inhibitors. Stress response pathways also help restore protein-folding homeostasis when environmental conditions inhibit translocation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kristoffer E. Johansson, Bayan Mashahreh, Rasmus Hartmann-Petersen, Tommer Ravid, Kresten Lindorff-Larsen
Summary: Effective proteome homeostasis is crucial for cell and organism survival. Cells have efficient quality control systems to monitor and remove misfolded proteins. The nature and sequence properties of quality-control degrons are still unknown.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Immunology
Xin Yan, Sen Hu, Yan Yang, Da Xu, Wenxing Liu, Ganwu Li, Wentong Cai, Zhigao Bu
Summary: Brucella, an intracellular pathogen, causes chronic infections in mammals. This study compared host responses to wild-type and Tat-mutant strains of Brucella melitensis M28. Proteomic analysis revealed increased production of immune response-related proteins in response to Tat mutant infection, indicating a role of these proteins in restricting Tat mutant survival within macrophages. Additionally, Tat mutants displayed higher sensitivity to nitrosative stress, and NO production was shown to contribute to limiting Tat mutant survival within macrophages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Te Liu, Yuying Liu, Zixuan Bu, Fan Yin, Yongqing Zhang, Jinjin Liu, Shaowen Li, Chen Tan, Xiabing Chen, Lu Li, Rui Zhou, Qi Huang
Summary: This study reveals the importance of the Tat system for bacterial motility in ExPEC, highlighting the role of transcriptional regulation in mediating motility defects, identifying Tat substrate proteins responsible for nonmotile phenotype, and showing the contribution of the Rcs system in suppressing motility.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Carolyn Allain Breckel, Mark Hochstrasser
Summary: The proper folding of proteins is vital for their diverse functions, and misfolded proteins can potentially harm cells by forming aggregates. Protein quality control pathways are responsible for repairing or degrading abnormal proteins, with the ubiquitin-proteasome system being commonly employed.
Review
Biochemistry & Molecular Biology
Mari A. Piirainen, Alexander D. Frey
Summary: Yeasts are widely used for biopharmaceutical production, but N-glycosylated biopharmaceuticals manufactured with yeasts are missing on the market. N-linked glycans play crucial roles in protein properties and folding, but current glycoengineering approaches face challenges.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Anthony H. Asher, Steven M. Theg
Summary: The mechanism and pore architecture of the Tat complex during transport of folded substrates remain a mystery, while the toroidal pore model better accounts for the high-energy barrier in Tat transport. The proteinaceous SecY pore undergoes conformational shifts between closed and opened states for translocating unfolded substrate chains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Selin Altinok, Rebekah Sanchez-Hodge, Mariah Stewart, Kaitlan Smith, Jonathan C. Schisler
Summary: Heat shock proteins (HSPs) are a crucial family of molecular chaperones that play a vital role in maintaining protein homeostasis by regulating protein refolding and triage decisions. Interactions between HSPs and co-chaperone proteins can impact various cellular functions, with dysfunctions in this machinery potentially contributing to a range of human diseases. Therapeutic targeting of co-chaperones is being explored as a precise treatment approach for diseases related to protein quality control defects, although challenges remain due to the broad functions of HSPs.
Article
Biochemistry & Molecular Biology
Hendrik Osadnik, Michael Schoepfel, Eyleen Heidrich, Denise Mehner, Hauke Lilie, Christoph Parthier, H. Jelger Risselada, Helmut Grubmueller, Milton T. Stubbs, Thomas Brueser
MOLECULAR MICROBIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Michael T. Ringel, Gerald Draeger, Thomas Brueser
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Patrick Stolle, Bo Hou, Thomas Brueser
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Claudia Rathmann, Amelie S. Schloesser, Juergen Schiller, Mikhail Bogdanov, Thomas Brueser
Article
Biochemistry & Molecular Biology
Michael T. Ringel, Gerald Draeger, Thomas Brueser
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Michael T. Ringel, Gerald Draeger, Thomas Brueser
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Bo Hou, Eyleen S. Heidrich, Denise Mehner-Breitfeld, Thomas Brueser
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Eyleen Sabine Heidrich, Thomas Brueser
Article
Microbiology
Denise Mehner-Breiffeld, Claudia Rathmann, Thomas Riedel, Ingo Just, Ralf Gerhard, Joerg Overmann, Thomas Brueser
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Microbiology
Hendrik Geise, Eyleen Sabine Heidrich, Christoph Stefan Nikolin, Denise Mehner-Breitfeld, Thomas Brueser
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lena Hoffmann, Michael-Frederick Sugue, Thomas Brueser
Summary: A new anthranilate-dependent P-antA promoter system for tunable gene expression in pseudomonads has been established, allowing for adjustable gene expression at lower or higher levels. This system was found to work not only in P. fluorescens but also in strains of P. aeruginosa and P. putida, making it potentially useful for a wide range of physiological and biotechnological applications in pseudomonads.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniel Valenzuela-Heredia, Carlos Henriquez-Castillo, Raul Donoso, Paris Lavin, Michael T. Ringel, Thomas Bruser, Jose Luis Campos
Summary: Members of the genus Pseudomonas are found in diverse environments, with varying genomes reflecting their habitats. The strain Pseudomonas sp. ABC1 has an abundance of iron hoarding mechanisms, making it a potential industrial microorganism.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Microbiology
Denise Mehner-Breitfeld, Jan Michel Frederik Schwarzkopf, Ry Young, Kiran Kondabagil, Thomas Brueser
Summary: The periplasmic antiholin RI is now found to have a cleavable signal peptide in its N-terminal trans-membrane domain, contrary to previous beliefs. This discovery suggests a fundamentally different interpretation of phage lysis regulation and the structure of the soluble domains of the RI-T complex.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michael-Frederick Sugue, Ali Nazmi Burdur, Michael T. Ringel, Gerald Draeger, Thomas Brueser
Summary: This study reveals the subcellular localization and functional role of PvdM. Using the model organism P. fluorescens, it is shown that PvdM is anchored to the periplasmic side of the cytoplasmic membrane, where it is essential for the activity of the tyrosinase PvdP. The results also suggest that PvdM prevents loss of the biosynthesis intermediate of pyoverdine into the medium by ensuring the efficient transfer of ferribactin to PvdP.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Cell Biology
Michael T. Ringel, Thomas Brueser