期刊
FEBS LETTERS
卷 584, 期 5, 页码 1041-1046出版社
WILEY
DOI: 10.1016/j.febslet.2010.02.005
关键词
SLO3; Capacitation; Sperm; Acrosome reaction
资金
- NIH [1R21HD056444-01A1, R01 NS0661871-01]
- CONACyT-Mexico [49113]
- DGAPA/UNAM [IN211809]
- Wellcome Trust
Here we show a unique example of male infertility conferred by a gene knockout of the sperm-specific, pH-dependent SLO3 potassium channel. In striking contrast to wild-type sperm which undergo membrane hyperpolarization during capacitation, we found that SLO3 mutant sperm undergo membrane depolarization. Several defects in SLO3 mutant sperm are evident under capacitating conditions, including impaired motility, a bent hairpin shape, and failure to undergo the acrosome reaction (AR). The failure of AR is rescued by valinomycin which hyperpolarizes mutant sperm. Thus SLO3 is the principal potassium channel responsible for capacitation-induced hyperpolarization, and membrane hyperpolarization is crucial to the AR. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据