Article
Biochemistry & Molecular Biology
Gregory Barshtein, Alexander Gural, Dan Arbell, Refael Barkan, Leonid Livshits, Ivana Pajic-Lijakovic, Saul Yedgar
Summary: Reduced deformability of red blood cells (RBCs) is associated with various circulatory disorders and anemias, and is linked to metabolic and structural alterations. This study identified 14 proteins that are positively correlated with RBC deformability, suggesting that the level of one or a few of these proteins can be used to assess cell deformability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
M. C. Berrevoets, J. Bos, R. Huisjes, T. H. Merkx, B. A. van Oirschot, W. W. van Solinge, J. W. Verweij, M. Y. A. Lindeboom, E. J. van Beers, M. Bartels, R. van Wijk, M. A. E. Rab
Summary: Hereditary spherocytosis is a common form of hereditary chronic hemolytic anemia. Splenectomy, which is often performed in HS, leads to improvements in RBC properties such as intracellular viscosity and membrane stability. The Cell Membrane Stability Test (CMST) may serve as a novel biomarker for reflecting RBC membrane health and treatment response in HS.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Urology & Nephrology
Rosi Bissinger, Travis Nemkov, Angelo D'Alessandro, Marijke Grau, Thomas Dietz, Bernhard N. Bohnert, Daniel Essigke, Matthias Woern, Lina Schaefer, Mengyun Xiao, Jonathan M. Beirne, M. Zaher Kalo, Anja Schork, Tamam Bakchoul, Kingsley Omage, Lingsi Kong, Irene Gonzalez-Menendez, Leticia Quintanilla-Martinez, Birgit Fehrenbacher, Martin Schaller, Achal Dhariwal, Andreas L. Birkenfeld, Florian Grahammer, Syed M. Qadri, Ferruh Artunc
Summary: This study using mouse models demonstrates the development of chronic kidney disease-associated anemia is linked to altered red blood cell metabolism, reduced RBC deformability, and enhanced cell death.
KIDNEY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Travis Nemkov, Sarah C. Skinner, Elie Nader, Davide Stefanoni, Melanie Robert, Francesca Cendali, Emeric Stauffer, Agnes Cibiel, Camille Boisson, Philippe Connes, Angelo D'Alessandro
Summary: A controlled, high-intensity cycling test resulted in decreased RBC deformability, increased microparticle generation, oxidative stress, and activation of membrane lipid remodeling mechanisms. Metabolites involved in CoA synthesis and carnitine conversion were significantly correlated with RBC properties during exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Yuncheng Man, Debnath Maji, Ran An, Sanjay P. Ahuja, Jane A. Little, Michael A. Suster, Pedram Mohseni, Umut A. Gurkan
Summary: This study introduces a novel microfluidic testing method, MIRCA, for standardized assessment of RBC-mediated microvascular occlusion. The technology shows promise in diseases like SCD and hereditary spherocytosis, correlating electrical impedance measurement with high-resolution imaging and clinical treatment outcomes. Impedance measurement obviates the need for high-resolution imaging, making it portable and suitable for point-of-care use in the future.
Article
Cell Biology
Claudia Bernecker, Eva Maria Matzhold, Dagmar Kolb, Afrim Avdili, Lisa Rohrhofer, Annika Lampl, Martin Troetzmueller, Heike Singer, Johannes Oldenburg, Peter Schlenke, Isabel Dorn
Summary: Functional characterization of cultured red blood cells from human induced pluripotent stem cells (cRBC_iPSCs) was conducted in this study. The results showed that cRBC_iPSCs have similar morphology and maturity stage to native red blood cells, highlighting their potential for future applications.
Article
Biochemistry & Molecular Biology
Dina Baier, Torsten Mueller, Thomas Mohr, Ursula Windberger
Summary: The study investigated the apparent Young's modulus and adhesion properties of different animal red blood cells, finding that the Young's modulus decreased with increasing temperature in all cell types, while adhesion increased with temperature in mammalian cells and correlated with reported membrane sialic acid concentrations.
Article
Cardiac & Cardiovascular Systems
Francesca Leo, Tatsiana Suvorava, Sophia K. Heuser, Junjie Li, Anthea LoBue, Frederik Barbarino, Eugenia Piragine, Rebekka Schneckmann, Beate Hutzler, Miranda E. Good, Bernadette O. Fernandez, Lukas Vornholz, Stephen Rogers, Allan Doctor, Maria Grandoch, Johannes Stegbauer, Eddie Weitzberg, Martin Feelisch, Jon O. Lundberg, Brant E. Isakson, Malte Kelm, Miriam M. Cortese-Krott
Summary: This study demonstrates that eNOS in ECs and RBCs independently contribute to blood pressure homeostasis. EC eNOS KO mice exhibited significantly impaired aortic dilatory responses to acetylcholine, loss of flow-mediated dilation, and increased blood pressure. On the other hand, RBC eNOS KO mice showed no alterations in acetylcholine-mediated dilation or flow-mediated dilation but were hypertensive.
Article
Biochemical Research Methods
Qiaodong Wei, Ying Xiong, Yuhang Ma, Deyun Liu, Yunshu Lu, Shenghong Zhang, Xiaolong Wang, Huaxiong Huang, Yingbin Liu, Ming Dao, Xiaobo Gong
Summary: The physical and mechanical properties of red blood cells (RBCs) play an important role in disease diagnosis, but it is challenging to measure these properties accurately due to the unique shape and high heterogeneity of RBCs. In this study, a microfluidic technology combined with machine learning is developed to measure the mechanical properties of individual RBCs. The method can detect subtle changes in RBC components in response to different doses of drugs and explore the correlation between glycosylated hemoglobin and RBC mechanical properties. This approach has the potential to offer novel label-free single-cell-assay-based biophysical markers for RBCs, enhancing the potential for more robust disease diagnosis.
Article
Multidisciplinary Sciences
Julian Eder, Leonie Schumm, Jakob P. P. Armann, Milo A. A. Puhan, Felix Beuschlein, Clemens Kirschbaum, Reinhard Berner, Nicole Toepfner
Summary: This study investigated the morphological and mechanical characteristics of red blood cells (RBCs) after SARS-CoV-2 infection in children and adolescents using real-time deformability-cytometry (RT-DC), and explored the relationship between RBC alterations and the clinical course of COVID-19. The results showed that the median RBC deformation was significantly increased in SARS-CoV-2-seropositive children and adolescents until 6 months post-COVID-19, indicating a mild course of the disease.
SCIENTIFIC REPORTS
(2023)
Article
Physiology
Michael Piagnerelli, Julie Vanderelst, Alexandre Rousseau, Daniel Monteyne, David Perez-Morga, Patrick Biston, Karim Zouaoui Boudjeltia
Summary: In patients with severe hypoxemic COVID-19, red blood cells (RBCs) showed normal deformability on admission, which remained unchanged over the first week despite changes in shape. This maintenance of normal RBC deformability may contribute to the adequate microcirculatory response to severe hypoxia observed in these patients.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Beata Csiszar, Gergely Galos, Simone Funke, Dora Kinga Kevey, Matyas Meggyes, Laszlo Szereday, Peter Kenyeres, Kalman Toth, Barbara Sandor
Summary: Our study investigated the maternal red blood cell properties in early-onset preeclampsia. We found that RBC aggregation was significantly elevated and RBC deformability reduced in women with PE. The AI and M index values showed potential for predicting early-onset PE, with a positive linear relationship observed between AI and gestational age at birth.
Article
Chemistry, Multidisciplinary
Tamir Tsohar, Shaul Beyth, Alexander Gural, Dan Arbell, Saul Yedgar, Gregory Barshtein
Summary: This study investigated the impact of non-physiological mechanical stress during surgery on red blood cell deformability. The findings showed that mechanical stress reduces cell deformability, increases the percentage of undeformable cells, and leads to hemolysis of rigid cells. The final outcome depends on the initial concentration of undeformable cells in the sample.
APPLIED SCIENCES-BASEL
(2022)
Article
Medicine, General & Internal
Julie Vanderelst, Alexandre Rousseau, Nicolas Selvais, Patrick Biston, Karim Zouaoui Boudjeltia, Michael Piagnerelli
Summary: The study found that during sepsis, there are changes in the shape and deformability of red blood cells, as well as a decrease in the expression of the CD35 membrane protein. These changes may contribute to the phagocytosis of red blood cells and the development of anemia in sepsis.
FRONTIERS IN MEDICINE
(2022)
Article
Biophysics
Bing -De Zheng, Mei-tian Xiao
Summary: Nano-RBMs is a disguised therapeutic platform that can resist recognition by the immune system and prolong the circulation time of the treatment agent in the blood, achieving ideal phototherapy effects.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Oncology
Yajing Gao, Pekka Paivinen, Sushil Tripathi, Eva Domenech-Moreno, Iris P. L. Wong, Kari Vaahtomeri, Ashwini S. Nagaraj, Sarang S. Talwelkar, Marc Foretz, Emmy W. Verschuren, Benoit Viollet, Yan Yan, Tomi P. Makela
Summary: This study reveals that in LKB1-mutant LUAD, inactivation of AMPK leads to immune evasion, attenuated antigen presentation, and the formation of an immune suppressive tumor microenvironment.
CLINICAL CANCER RESEARCH
(2022)
Editorial Material
Medicine, Research & Experimental
Severine Olivier, Hanna Diounou, Marc Foretz, Sandra Guilmeau, Noemie Daniel, Andre Marette, Malvyne Rolli-Derkinderen, Benoit Viollet
M S-MEDECINE SCIENCES
(2022)
Article
Cell Biology
Severine Olivier, Hanna Diounou, Camille Pochard, Lisa Frechin, Emilie Durieu, Marc Foretz, Michel Neunlist, Malvyne Rolli-Derkinderen, Benoit Viollet
Summary: Dysfunctions in the intestinal barrier are commonly observed in inflammatory bowel disease (IBD) and the AMP-activated protein kinase (AMPK) plays a key role in the stabilization and assembly of tight junctions. This study investigated the contribution of intestinal epithelial AMPK to acute colitis and found that its deletion delayed intestinal injury repair and impaired barrier function. Metformin administration improved intestinal repair capacity independently of AMPK. These findings highlight the importance of AMPK in regulating mucosal repair and suggest the therapeutic potential of metformin in supporting the repair of injured intestinal epithelium.
Article
Obstetrics & Gynecology
Pascal Froment, Ingrid Plotton, Cecilia Giulivi, Stephane Fabre, Rita Khoueiry, Nizar Mourad, Sandrine Horman, Christelle Rame, Charlene Rouillon, Jeremy Grandhaye, Yves Bigot, Claire Chevaleyre, Remy Le Guevel, Patricia Mallegol, Ramaroson Andriantsitohaina, Fabrice Guerif, Jerome Tamburini, Benoit Viollet, Marc Foretz, Joelle Dupont
Summary: The lack of alpha 1AMPK in granulosa cells impacts various biological processes and leads to a hyperandrogenic response.
HUMAN REPRODUCTION
(2022)
Article
Nutrition & Dietetics
Tristan Chalvon-Demersay, Claire Gaudichon, Joanna Moro, Patrick C. Even, Nadezda Khodorova, Julien Piedcoq, Benoit Viollet, Julien Averous, Anne-Catherine Maurin, Daniel Tome, Marc Foretz, Pierre Fafournoux, Dalila Azzout-Marniche
Summary: This study investigated the contribution of liver AMPK and GCN2 to the adaptation to high protein intake and found that they were not involved in this adaptation during the postprandial period.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Multidisciplinary Sciences
Javier Moral-Sanz, Sophronia A. Lewis, Sandy MacMillan, Marco Meloni, Heather McClafferty, Benoit Viollet, Marc Foretz, Jorge del-Pozo, A. Mark Evans
Summary: This study shows that deficiency of AMPK-alpha 1/alpha 2 in smooth muscles can cause persistent pulmonary hypertension in newborns. The findings provide important insights into the mechanism of pulmonary hypertension.
NATURE COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Iain R. Phair, Raid B. Nisr, Andrew J. M. Howden, Magdalena Sovakova, Noor Alqurashi, Marc Foretz, Douglas Lamont, Benoit Viollet, Graham Rena
Summary: This study demonstrates the integration of metabolite and kinase-based immunometabolic control in macrophages through the activation of AMP kinase. The deletion of AMPKa1 leads to a striking polarization of M1 macrophages upon LPS stimulation, along with increased expression of rate-limiting enzymes involved in various metabolic pathways.
MOLECULAR METABOLISM
(2022)
Review
Endocrinology & Metabolism
Marc Foretz, Bruno Guigas, Benoit Viollet
Summary: This Review presents the latest advances in understanding the mechanisms of action of metformin and discusses its potential repurposing for other indications. Metformin is currently the first-line medication for treating type 2 diabetes in most guidelines and is widely used by over 200 million patients. Its therapeutic action involves complex mechanisms that are not fully understood. Although the liver was initially identified as the main organ involved, increasing evidence suggests the involvement of other sites, including the gastrointestinal tract, gut microbial communities, and tissue-resident immune cells. The molecular mechanisms of metformin vary depending on the dose and duration of treatment, and recent studies have revealed a novel action on the lysosome surface at low concentrations. Considering its efficacy and safety in diabetes, attention has been given to using metformin as adjunct therapy for cancer, age-related diseases, inflammatory diseases, and COVID-19.
NATURE REVIEWS ENDOCRINOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Miroslava Kvandova, Sanela Rajlic, Paul Stamm, Isabella Schmal, Dominika Mihalikova, Marin Kuntic, Maria Teresa Bayo Jimenez, Omar Hahad, Marta Kollarova, Henning Ubbens, Lea Strohm, Katie Frenis, Georg Daniel Duerr, Marc Foretz, Benoit Viollet, Yue Ruan, Subao Jiang, Qi Tang, Hartmut Kleinert, Steffen Rapp, Adrian Gericke, Eberhard Schulz, Matthias Oelze, John F. Keaney, Andreas Daiber, Swenja Kroller-Schon, Thomas Jansen, Thomas Munzel
Summary: This study found that the activation of adenosine monophosphate-activated protein kinase (a1AMPK) through exercise, intermittent fasting, and pharmacological treatment effectively mitigates vascular dysfunction caused by aircraft noise. These findings provide a new approach for the prevention and treatment of transportation noise-associated diseases.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Multidisciplinary Sciences
Anne Garnier, Jerome Leroy, Claudine Delomenie, Philippe Mateo, Benoit Viollet, Vladimir Veksler, Mathias Mericskay, Renee Ventura-Clapier, Jerome Piquereau
Summary: The study explores the potential control of the beta-adrenergic pathway by AMP-activated protein kinase (AMPK) in the context of compromised energy metabolism. The activation of AMPK was found to down-regulate the expression of adenylyl cyclase 5 (AC5) and blunt the beta-adrenergic cascade, suggesting a compensatory energy sparing mechanism in dysfunctional myocardium.
Meeting Abstract
Cardiac & Cardiovascular Systems
Justine Dontaine, Laurent Bultot, Natacha Fourny, Laura Gilbert, Roselle Gelinas, Florence Mailleux, Audrey Ginion, Evangelos-Panagiotis Daskalopoulos, Hrag Esfahani, Didier Vertommen, Jean-Luc Balligand, Benoit Viollet, Christophe Beauloye, Sandrine Horman, Luc Bertrand
Meeting Abstract
Biophysics
Malgorzata Tokarska-Schlattner, Laurence Kay, Pascale Perret, Raffaella Isola, Stephane Attia, Frederic Lamarche, Cindy Tellier, Cecile Cottet-Rousselle, Amjad Uneisi, Marc Foretz, Herve Dubouchaud, Catherine Ghezzi, Christian Zuppinger, Benoit Viollet, Uwe Schlattner
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
J. Dontaine, L. Bultot, N. Fourny, L. Guilbert, R. Gelinas, F. Mailleux, E. P. Daskalopoulos, H. Esfahani, J-L Balligand, B. Viollet, C. Beauloye, S. Horman, L. Bertrand
EUROPEAN JOURNAL OF HEART FAILURE
(2022)
Article
Gastroenterology & Hepatology
Pascale Gluais-Dagorn, Marc Foretz, Gregory R. Steinberg, Battsetseg Batchuluun, Anna Zawistowska-Deniziak, Joost M. Lambooij, Bruno Guigas, David Carling, Pierre-Axel Monternier, David E. Moller, Sebastien Bolze, Sophie Hallakou-Bozec
Summary: The study demonstrates that direct activation of AMPK can improve all core components of NASH and alleviate related hyperglycemia, dyslipidemia, and systemic inflammation. Additionally, novel properties of direct AMPK activation were revealed, including improved insulin resistance and direct suppression of inflammation and fibrogenesis. These findings suggest the potential for direct AMPK activation as an effective treatment for patients with NASH, with effects also observed in human cells.
HEPATOLOGY COMMUNICATIONS
(2022)
